Nematodes cannot hide their spots: in vivo proteomic approaches to understanding the mechanisms of drug resistance in nematodes of veterinary importance

PM Brophy, J Barrett & W Haresign

The project will experimentally examine the hypothesis that the in vivo based capture of drug target complexes and associated proteins in wild-type and drug resistant strains of the model laboratory nematode model C. elegans will identify a pool of resistance routes that can be transferred to resistance monitoring in related heterogeneous GI nematode field population of livestock under different drug selection pressure. The project will specifically address the following questions: 1) How do glutamate-gated chloride-channel subunit and associated protein interactions in wild type and IVM resistant C. elegans change on IVM exposure? and 2) Can C. elegans identify and validate resistance monitoring markers for IVM in GI nematodes of veterinary importance?