Dr Amanda Lloyd BSc: Genetics and biochemistry PhD: Metabolomics and plant pathology
Together with Prof John Draper and Dr Manfred Beckmann I am interested in developing standardised protocols for nutritional metabolomics investigations for monitoring metabolic changes in response to both acute and habitual dietary exposure. Techniques employed includes high throughput metabolomic fingerprinting based on Flow Infusion Electrospray Ionization-Mass Spectrometry (FIE-MS), accurate mass analysis using Fourier-Transform Ion Cyclotron Resonance Ultra MS (FT-ICR-MS) in conjunction with supervised multivariate data analysis (Principal Components Analysis, Linear Discriminant Analysis, Area Under the (Receiver operating characteristic) curve (AUC), and Random Forest) and ESI-MS signal annotation tools (MZedDB).
Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets: Plasma lipidome of dog breeds using UHPLC-HRM. Metabolomics 13 15 10.1007/s11306-016-1152-0 Cadair2017.
Developing community-based urine sampling methods to facilitate dietary exposure biomarker technology for population assessment. Proceedings of the Nutrition Society 75 (OCE3) E240 Nutrition Society Summer Meeting. 10.1017/S002966511600255X Cadair2016.
Quantification of dietary biomarkers in spot urine samples reflects the intake of foods of UK high public health importance. Proceedings of the Nutrition Society 75 (OCE3) E248 Nutrition Society Summer Meeting. 10.1017/S0029665116002639 Cadair2016.
Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intake. Molecular Nutrition and Food Research 60 (2) pp. 444-457. 10.1002/mnfr.201500495 Cadair2016.
Hydroxylated phenylacetamides derived from bioactive benzoxazinoids are bioavailable in humans after habitual consumption of whole grain sourdough rye bread. Molecular Nutrition and Food Research 57 (10) pp. 1859-1873. 10.1002/mnfr.201200777 Cadair2013.
Flow infusion electrospray ionisation mass spectrometry for high throughput, non-targeted metabolite fingerprinting: A Review. Metabolomics 9 (1 Supplement) pp. S4–S29. 10.1007/s11306-012-0449-x Cadair2013.
Separating the inseparable: The metabolomic analysis of plant-pathogen interactions. In (eds) Plant Metabolomics : Methods and Protocols. Methods in Molecular Biology Cadair2012.
Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption. British Journal of Nutrition 106 (6) pp. 812-824. 10.1017/S0007114511001164 Cadair2011.
Metabolomic approaches indicate that cell wall modifications play a major role in ethylene-mediated resistance against Botrytis cinerea. Plant Journal 67 (5) pp. 852-868. 10.1111/j.1365-313X.2011.04639.x Cadair2011.
Metabolomic approaches to understand ethylene mediated defenses in Arabidopsis thaliana against Botrytis cinerea. In (eds) Advances in Plant Ethylene Research. Springer Nature, Dordrecht pp. 359-368. 10.1007/978-1-4020-6014-4_77 Cadair2007.