Dr Amanda Lloyd BSc: Genetics and biochemistry PhD: Metabolomics and plant pathology

Dr Amanda Lloyd

Research Assistant

Contact Details

Research

Together with Prof John Draper and Dr Manfred Beckmann I am interested in developing standardised protocols for nutritional metabolomics investigations for monitoring metabolic changes in response to both acute and habitual dietary exposure. Techniques employed includes high throughput metabolomic fingerprinting based on Flow Infusion Electrospray Ionization-Mass Spectrometry (FIE-MS), accurate mass analysis using Fourier-Transform Ion Cyclotron Resonance Ultra MS (FT-ICR-MS) in conjunction with supervised multivariate data analysis (Principal Components Analysis, Linear Discriminant Analysis, Area Under the (Receiver operating characteristic) curve (AUC), and Random Forest) and ESI-MS signal annotation tools (MZedDB).

Publications

2017

Woodside, J.V., Draper, J., Lloyd, A., McKinley, M.C. 2017. Use of biomarkers to assess fruit and vegetables intake. Proceedings of the Nutrition Society Nutrition Society Summer Meeting. 10.1017/S0029665117000325 Cadair

Lloyd, A., Beckmann, M., Wilson, T., Tailliart, K., Allaway, D., Draper, J. 2017. Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets: Plasma lipidome of dog breeds using UHPLC-HRM. Metabolomics 13 15 10.1007/s11306-016-1152-0 Cadair

2016

Wilson, T., Willis, N.D., Zubair, H., Beckmann, M., Xie, L., Tailliart, K., Lloyd, A., Mathers, J.C., Draper, J. 2016. Developing community-based urine sampling methods to facilitate dietary exposure biomarker technology for population assessment. Proceedings of the Nutrition Society 75 (OCE3) E240 Nutrition Society Summer Meeting. 10.1017/S002966511600255X Cadair

Lloyd, A.J., Zubair, H., Willis, N.D., Wilson, T., Xie, L., Tailliart, K., Chambers, E.S., Garcia-Perez, I., Holmes, E., Frost, G., Mathers, J.C., Beckmann, M., Draper, J. 2016. Quantification of dietary biomarkers in spot urine samples reflects the intake of foods of UK high public health importance. Proceedings of the Nutrition Society 75 (OCE3) E248 Nutrition Society Summer Meeting. 10.1017/S0029665116002639 Cadair

Lloyd, A., Beckmann, M., Tailliart, K., Brown, W.Y., Draper, J., Allaway, D. 2016. Characterisation of the main drivers of intra- and inter- breed variability in the plasma metabolome of dogs. Metabolomics 12 (4) 72 10.1007/s11306-016-0997-6 Cadair

Beckmann, M., Joosen, A.M., Clarke, M.M., Mugridge, O., Frost, G., Engel, B., Tailliart, K., Lloyd, A., Draper, J., Lodge, J.K. 2016. Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intake. Molecular Nutrition and Food Research 60 (2) pp. 444-457. 10.1002/mnfr.201500495 Cadair

2013

Beckmann, M., Lloyd, A.J., Halder, S., Seal, C., Brandt, K., Draper, J. 2013. Hydroxylated phenylacetamides derived from bioactive benzoxazinoids are bioavailable in humans after habitual consumption of whole grain sourdough rye bread. Molecular Nutrition and Food Research 57 (10) pp. 1859-1873. 10.1002/mnfr.201200777 Cadair

Beckmann, M., Lloyd, A., Halder, S., Fave, G., Seal, C., Brandt, K., Mathers, J.C., Draper, J. 2013. Dietary exposure biomarker-lead discovery based on Metabolomics analysis of urine samples. Proceedings of the Nutrition Society 72 (3) pp. 352-361. 10.1017/S0029665113001237 Other Cadair

Draper, J., Lloyd, A.J., Goodacre, R., Beckmann, M. 2013. Flow infusion electrospray ionisation mass spectrometry for high throughput, non-targeted metabolite fingerprinting: A Review. Metabolomics 9 (1 Supplement) pp. S4–S29. 10.1007/s11306-012-0449-x Cadair

Lloyd, A.J., Beckmann, M., Haldar, S., Seal, C., Brandt, K., Draper, J. 2013. Data-driven strategy for the discovery of potential urinary biomarkers of habitual dietary exposure. American Journal of Clinical Nutrition 97 (2 ) pp. 377-389. 10.3945/ajcn.112.048033 Cadair

2012

Rodríguez-López, C.M., Lloyd, A.J., Leonard, K., Wilkinson, M.J. 2012. Differential Effect of Three Base Modifications on DNA Thermostability Revealed by High Resolution Melting. Analytical Chemistry 84 (17) pp. 7336-7342. 10.1021/ac301459x Cadair

Allwood, J.W., Heald, J., Lloyd, A.J., Goodacre, R., Mur, L.A.J. 2012. Separating the inseparable: The metabolomic analysis of plant-pathogen interactions. In N. W. Hardy., R. D. Hall. (eds) Plant Metabolomics : Methods and Protocols. Methods in Molecular Biology Cadair

2011

Favé, G., Beckmann, M., Lloyd, A., Zhou, S., Harold, G., Lin, W., Tailliart, K., Xie, L., Draper, J., Mathers, J.C. 2011. Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples. Metabolomics 7 (4) pp. 469-484. 10.1007/s11306-011-0289-0 Cadair

Lewis, P.D., Lewis, K.E., Ghosal, R., Bayliss, S., Lloyd, A.J., Wills, J., Godfrey, R., Kloer, P., Mur, L.A.J. 2011. Evaluation of FTIR Spectroscopy as a diagnostic tool for lung cancer using sputum. BMC Cancer 10 (640) 640 10.1186/1471-2407-10-640 Cadair

Lloyd, A.J., Beckmann, M., Favé, G., Mathers, J.C., Draper, J. 2011. Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption. British Journal of Nutrition 106 (6) pp. 812-824. 10.1017/S0007114511001164 Cadair

Lloyd, A.J., Allwood, J.W., Winder, C.L., Dunn, W.B., Heald, J.K., Cristescu, S.M., Sivakumaran, A., Harren, F.J.M., Mulema, J., Denby, K., Goodacre, R., Smith, A.R., Mur, L.A.J. 2011. Metabolomic approaches indicate that cell wall modifications play a major role in ethylene-mediated resistance against Botrytis cinerea. Plant Journal 67 (5) pp. 852-868. 10.1111/j.1365-313X.2011.04639.x Cadair

Lloyd, A.J., Favé, G., Beckmann, M., Lin, W., Tailliart, K., Xie, L., Mathers, J.C., Draper, J. 2011. Use of mass spectrometry fingerprinting to identify urinary metabolites after consumption of specific foods. American Journal of Clinical Nutrition 94 (4) pp. 981-991. 10.3945/ajcn.111.017921 Cadair

Lloyd, A.J., Wilkinson, M.J., Guzmán Asenjo, B., Rodriguez Lopez, C.M. 2011. Direct detection and quantification of methylation in nucleic acid sequences using high resolution melting analysis. Analytical Chemistry 82 (21) pp. 9100-9108. 10.1021/ac1024057 Cadair

2008

Mur, L.A.J., Lloyd, A.J., Cristescu, S.M., Harren, F.J.M., Hall, M., Smith, A. 2008. Biphasic ethylene production during the hypersensitive response in Arabidopsis: A window into defence priming mechanisms? Plant Signaling and Behavior 4 (7) pp. 610-613. 10.4161/psb.4.7.8904 Cadair

2007

Johnson, H.E., Smith, A.R., Mur, L.A.J., Causton, D.R., Lloyd, A.J. 2007. The application of MANOVA to analyse Arabidopsis thaliana metabolomic data from factorially designed experiments. Metabolomics pp. 517-530. 10.1007/s11306-007-0065-3 Cadair

Lloyd, A.J., Smith, A.R., Mur, L.A.J. 2007. Metabolomic approaches to understand ethylene mediated defenses in Arabidopsis thaliana against Botrytis cinerea. In A. Ramina., C. Chang., J. Giovannoni., H. Klee., P. Perata., E. Woltering. (eds) Advances in Plant Ethylene Research. Springer Nature, Dordrecht pp. 359-368. 10.1007/978-1-4020-6014-4_77 Cadair

Mur, L.A.J., Kenton, P., Lloyd, A.J., Ougham, H.J., Prats, E. 2007. The hypersensitive response; the centenary is upon us but how much do we know? Journal of Experimental Botany 59 (3) pp. 501-520. 10.1093/jxb/erm239 Cadair