Dr Amanda Lloyd

BSc: Genetics and biochemistry PhD: Metabolomics and plant pathology

Dr Amanda Lloyd

Research Assistant

Contact Details

Research

Together with Prof John Draper and Dr Manfred Beckmann I am interested in developing standardised protocols for nutritional metabolomics investigations for monitoring metabolic changes in response to both acute and habitual dietary exposure. Techniques employed includes high throughput metabolomic fingerprinting based on Flow Infusion Electrospray Ionization-Mass Spectrometry (FIE-MS), accurate mass analysis using Fourier-Transform Ion Cyclotron Resonance Ultra MS (FT-ICR-MS) in conjunction with supervised multivariate data analysis (Principal Components Analysis, Linear Discriminant Analysis, Area Under the (Receiver operating characteristic) curve (AUC), and Random Forest) and ESI-MS signal annotation tools (MZedDB).

Publications

Use of biomarkers to assess fruit and vegetables intakeWoodside, J. V., Draper, J., Lloyd, A. & McKinley, M. C. 2017 In : Proceedings of the Nutrition Society.76, 3, p. 308-3158 p.
Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets: Plasma lipidome of dog breeds using UHPLC-HRMLloyd, A., Beckmann, M., Wilson, T., Tailliart, K., Allaway, D. & Draper, J. 2017 In : Metabolomics.13, 15
Developing community-based urine sampling methods to facilitate dietary exposure biomarker technology for population assessmentWilson, T., Willis, N. D., Zubair, H., Beckmann, M., Xie, L., Tailliart, K., Lloyd, A., Mathers, J. C. & Draper, J. 2016 In : Proceedings of the Nutrition Society.75, OCE3, E240
Quantification of dietary biomarkers in spot urine samples reflects the intake of foods of UK high public health importanceLloyd, A. J., Zubair, H., Willis, N. D., Wilson, T., Xie, L., Tailliart, K., Chambers, E. S., Garcia-Perez, I., Holmes, E., Frost, G., Mathers, J. C., Beckmann, M. & Draper, J. 2016 In : Proceedings of the Nutrition Society.75, OCE3, E248
Characterisation of the main drivers of intra- and inter- breed variability in the plasma metabolome of dogsLloyd, A., Beckmann, M., Tailliart, K., Brown, W. Y., Draper, J. & Allaway, D. 2016 In : Metabolomics.12, 4, 72
Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intakeBeckmann, M., Joosen, A. M., Clarke, M. M., Mugridge, O., Frost, G., Engel, B., Tailliart, K., Lloyd, A., Draper, J. & Lodge, J. K. 2016 In : Molecular Nutrition and Food Research.60, 2, p. 444-457
Hydroxylated phenylacetamides derived from bioactive benzoxazinoids are bioavailable in humans after habitual consumption of whole grain sourdough rye breadBeckmann, M., Lloyd, A. J., Halder, S., Seal, C., Brandt, K. & Draper, J. 2013 In : Molecular Nutrition and Food Research.57, 10, p. 1859-187315 p.
Dietary exposure biomarker-lead discovery based on Metabolomics analysis of urine samplesBeckmann, M., Lloyd, A., Halder, S., Fave, G., Seal, C., Brandt, K., Mathers, J. C. & Draper, J. 2013 In : Proceedings of the Nutrition Society.72, 3, p. 352-36110 p.
Flow infusion electrospray ionisation mass spectrometry for high throughput, non-targeted metabolite fingerprinting: A ReviewDraper, J., Lloyd, A. J., Goodacre, R. & Beckmann, M. 2013 In : Metabolomics.9, 1 Supplement, p. S4–S2926 p.
Data-driven strategy for the discovery of potential urinary biomarkers of habitual dietary exposureLloyd, A. J., Beckmann, M., Haldar, S., Seal, C., Brandt , K. & Draper, J. 2013 In : American Journal of Clinical Nutrition.97 , 2 , p. 377-38912 p.
Differential Effect of Three Base Modifications on DNA Thermostability Revealed by High Resolution MeltingRodríguez-López, C. M., Lloyd, A. J., Leonard, K. & Wilkinson, M. J. 2012 In : Analytical Chemistry.84, 17, p. 7336-73427 p.
Separating the inseparable: The metabolomic analysis of plant-pathogen interactionsAllwood, J. W., Heald, J., Lloyd, A. J., Goodacre, R. & Mur, L. A. J. 2012 Plant Metabolomics : Methods and Protocols. Hardy, N. W. & Hall, R. D. (eds.). (Methods in Molecular Biology)
Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samplesFavé, G., Beckmann, M., Lloyd, A., Zhou, S., Harold, G., Lin, W., Tailliart, K., Xie, L., Draper, J. & Mathers, J. C. 2011 In : Metabolomics.7, 4, p. 469-48416 p.
Evaluation of FTIR Spectroscopy as a diagnostic tool for lung cancer using sputumLewis, P. D., Lewis, K. E., Ghosal, R., Bayliss, S., Lloyd, A. J., Wills, J., Godfrey, R., Kloer, P. & Mur, L. A. J. 2011 In : BMC Cancer.10, 640, 640
Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumptionLloyd, A. J., Beckmann, M., Favé, G., Mathers, J. C. & Draper, J. 2011 In : British Journal of Nutrition.106, 6, p. 812-82413 p.
Metabolomic approaches indicate that cell wall modifications play a major role in ethylene-mediated resistance against Botrytis cinereaLloyd, A. J., Allwood, J. W., Winder, C. L., Dunn, W. B., Heald, J. K., Cristescu, S. M., Sivakumaran, A., Harren, F. J. M., Mulema, J., Denby, K., Goodacre, R., Smith, A. R. & Mur, L. A. J. 2011 In : Plant Journal.67, 5, p. 852-86817 p.
Use of mass spectrometry fingerprinting to identify urinary metabolites after consumption of specific foodsLloyd, A. J., Favé, G., Beckmann, M., Lin, W., Tailliart, K., Xie, L., Mathers, J. C. & Draper, J. 2011 In : American Journal of Clinical Nutrition.94, 4, p. 981-99111 p.
Direct detection and quantification of methylation in nucleic acid sequences using high resolution melting analysisLloyd, A. J., Wilkinson, M. J., Guzmán Asenjo, B. & Rodriguez Lopez, C. M. 2011 In : Analytical Chemistry.82, 21, p. 9100-91089 p.
Biphasic ethylene production during the hypersensitive response in Arabidopsis: A window into defence priming mechanisms?Mur, L. A. J., Lloyd, A. J., Cristescu, S. M., Harren, F. J. M., Hall, M. & Smith, A. 2009 In : Plant Signaling and Behavior.4, 7, p. 610-6134 p.
The hypersensitive response; the centenary is upon us but how much do we know?Mur, L. A. J., Kenton, P., Lloyd, A. J., Ougham, H. J. & Prats, E. 2007 In : Journal of Experimental Botany.59, 3, p. 501-52020 p.
The application of MANOVA to analyse Arabidopsis thaliana metabolomic data from factorially designed experimentsJohnson, H. E., Smith, A. R., Mur, L. A. J., Causton, D. R. & Lloyd, A. J. 2007 In : Metabolomics.p. 517-53014 p.
Metabolomic approaches to understand ethylene mediated defenses in Arabidopsis thaliana against Botrytis cinereaLloyd, A. J., Smith, A. R. & Mur, L. A. J. 2007 Advances in Plant Ethylene Research. Ramina, A., Chang, C., Giovannoni, J., Klee, H., Perata, P. & Woltering, E. (eds.). Dordrecht: Springer Nature, p. 359-368 77