Dr Ana Winters

Dr Ana Winters

Principal Investigator

Contact Details

Teaching

Research

I am a plant biochemist with a special interest in protein and phenol chemistry. My more recent work has focussed on the enzyme polyphenol oxidase in forage crops looking at gene expression, enzyme biochemistry and phenol substrate chemistry. I am currently studying the effects of post-harvest changes on proscessing of crops for biofuel.

Publications

What cell wall components are the best indicators for Miscanthus digestibility and conversion to ethanol following variable pretreatments?Adams, J., Winters, A., Hodgson, E. & Gallagher, J. 2018 In : Biotechnology for Biofuels.11, 14 p., 67
Novel rapid method for the characterisation of polymeric sugars from macroalgaeSpicer, S., Adams, J., Thomas, D., Gallagher, J. & Winters, A. 2017 In : Journal of Applied Phycology.29, 3, p. 1507-15137 p.
Could Miscanthus replace maize as the preferred substrate for anaerobic digestion in the United Kingdom?: Future breeding strategiesPurdy, S., Maddison, A., Nunn, C., Winters, A., Timms-Taravella, E., Jones, C., Clifton-Brown, J., Donnison, I. & Gallagher, J. 2017 In : GCB Bioenergy.9, 6, p. 1122-113917 p.
Aphid orientation and performance in glasshouses under different UV-A/UV-B radiation regimesDader, B., Moreno, A., Jones, D., Winters, A. & Fereres, A. 2017 In : Entomologia Experimentalis et Applicata.163, 3, p. 344-3539 p.
Polyphenols from Allanblackia floribunda seeds: Identification, quantification and antioxidant activityAkpanika, G. A., Winters, A., Wilson, T., Ayoola, G. A., Adepoju-Bello, A. A. & Hauck, B. 2017 In : Food Chemistry.222, p. 35-428 p.
Detection of potential chloroplastic substrates for polyphenol oxidase suggests a role in undamaged leavesBoeckx, T., Winters, A., Webb, K. & Kingston-Smith, A. 2017 In : Frontiers in Plant Science.8, 3, 237
A Cell wall Reference Profile for Miscanthus Bioenergy-crops Highlights Compositional and Structural Variations Associated with Development and Organ Originda Costa, R., Pattathil, S., Avci, U., Lee, S. J., Hazen, S. P., Winters, A., Hahn, M. & Bosch, M. 2017 In : New Phytologist.213, 4, p. 1710-1725
An efficient assay for ferulic acid esterase and para-coumaric acid esterases in plants at physiologically relevant activitiesShah, I., Donnison, I., Insole, J., Winters, A., Dalton, S., Woodman, T., Bosch, M. & Threadgill, M. D. 2016 (Accepted/In press) In : Cell Chemistry and Biology.6, 5, p. 300-31111 p.
Biodegradtion of different genotypes of Miscanthus by Wood Rot FungiBaker, P., Winters, A. & Hale, M. D. C. 2016 In : BioResources.11, 2, p. 4379-439113 p.
The functional quality of decomposing litter outputs from an Arctic plant community is affected by long-term exposure to enhanced UV-B. Ecological Indicators.Jones, A. G., Bussell, J. S., Winters, A., Scullion, J. & Jones, D. 2016 In : Ecological Indicators.60, p. 8-1710 p.
The development of tea blister caused by Exobasidium vexans in tea (Camellia sinensis) correlates with the reduced accumulation of some antimicrobial metabolites and the defence signals salicylic and jasmonic acidsMur, L., Hauck, B., Winters, A., Heald, J. K., Lloyd, A. J., Chakraborty, U. & Chakraborty, B. N. 2015 In : Plant Pathology.64, 6, p. 1471-1483
Effects of Plant Species, Stage of Maturity, and Level of Formic Acid Addition on Lipolysis, Lipid Content, and Fatty Acid Composition during Ensiling.koivonen, E., Jaakkola, S., Terttu, H., Lampi, A-M., Halmemies-Beauchet-Filleau, A., Lee, M. R., Winters, A., Shingfield, K. & Vanhatalo, A. 2015 In : Journal of Animal Science.93, p. 4408-4423
Polyphenol oxidase-mediated protection against oxidative stress is not associated with enhanced photosynthetic efficiencyBoeckx, T., Webster, R., Winters, A., Webb, J., Gay, A. & Kingston-Smith, A. 2015 In : Annals of Botany.116, 4, p. 529-54012 p.
Polyphenol oxidase in leaves; is there any significance to the chloroplastic localization?Boeckx, T., Winters, A., Webb, J. & Kingston-Smith, A. 2015 In : Journal of Experimental Botany.66, 12, p. 3571-3579
Polyphenol oxidase and red cloverWebb, J. & Winters, A. 2015 (Accepted/In press) In : Legume Perspectives.
Polyphenol oxidase affects normal nodule development in red clover (Trifolium pratense L.)Webb, J., Cookson, A., Allison, G., Sullivan, M. & Winters, A. 2014 In : Frontiers in Plant Science.5, 700
Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop MiscanthusFernandes Da Costa, R. M., Lee, S. J., Allison, G. G., Hazen, S. P., Winters, A. & Bosch, M. 2014 In : Annals of Botany.114, 6, p. 1265-127713 p.
Impact of UV-A radiation on the performance of aphids and whiteflies and on the leaf chemistry of their host plants.Dader, B., Jones, D., Moreno , . A., Winters, A. & Ferres, . A. 2014 In : Journal of Photochemistry and Photobiology B: Biology.138, p. 307-31610 p.
Screening for potential co-products in a Miscanthus sinensis mapping family by liquid chromatography with mass spectrometry detectionParveen, I., Wilson, T., Threadgill, M. D., Luyten, J., Roberts, R. E., Robson, P. R., Hauck, B. D., Donnison, I. S. & Winters, A. L. 2014 In : Phytochemistry.105, p. 186-19610 p.
Ruminal Prevotella spp. May Play an Important Role in the Conversion of Plant Lignans into Human Health Beneficial AntioxidantsSchogor, A. L. B., Huws, S. A., Santos, G. T. D., Scollan, N. D., Hauck, B. D., Winters, A. L., Kim, E. J., Petit, H. V. & Heimesaat, M. M. (ed.) 2014 In : PLoS One.9, 4, e87949
GB Application - Bioethanol from grassBryant, D., Morris, S. M., Gallagher, J., Winters, A., Donnison, I., Morris, P., Harding, G., Timms-Taravella, E., Thomas, D. & Leemans, D. 2014
Soluble Phenolic Compounds in Fresh and Ensiled Orchard Grass (Dactylis glomerata L.), a Common Species in Permanent Pastures with Potential as a Biomass FeedstockHauck, B., Gallagher, J., Morris, S. M., Leemans, D. K. & Winters, A. 2014 In : Journal of Agricultural and Food Chemistry.62, 2, p. 468-4758 p.
A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based on salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolismSanchez-Martin, J., Heald, J., Kingston-Smith, A., Winters, A., Rubiales, D., Sanz, M., Mur, L. & Prats, E. 2014 In : Plant, Cell and Environment.
Cell-wall compositional analysis of the energy crop Miscanthus as a means to optimise its application as a feedstock for bioenergy and biorefiningFernandes Da Costa, R. M., Winters, A. L. & Bosch, M. 2013 p. O6-03
Latitudinal variation In ambient UV-B radiation is an important determinant of Lolium perenne forage production, quality, and digestibilityComont, D. C., Winters, A. L., Gomez, . L. D., McQueen-Mason, S. J. & Gwynn-Jones, D. 2013 In : Journal of Experimental Botany.64, 8, p. 2193-2204
Gene expression patterns, localisation and substrates of polyphenol oxidase in red clover (Trifolium pratense L.)Webb, K. J., Cookson, A. R., Allison, G. G., Sullivan, M. L. & Winters, A. L. 2013 In : Journal of Agricultural and Food Chemistry.61, 31, p. 7421-743010 p.
Characterization of polyphenol oxidase (PPO) activity in a range of forage ecotypes with different phenol substrates. A new insight for PPO and protein bound phenol evaluationCabiddu, A., Lee, M. R., Decandia, M., Molle, G., Salis, L., Varqui, M. & Winters, A. L. 2013 In : Grass and Forage Science.69, 4, p. 678-69215 p.
Co-expression of lupanine hydroxylase and pyrroloquinoline quinone 2 leads to assembled and active recombinant lupanine hydroxylase in the 3 Escherichia coli periplasmStampolidis, P., Kaderbhai, N., Bryant, D., Winters, A., Gallagher, J. & Kaderbhai, M. A. 2013 In : Archives of Microbiology.
Enhanced UV-B and Elevated CO2 Impacts Sub-Arctic Shrub Berry Abundance, Quality and Seed GerminationGwynn-Jones, D., Jones, A. G., Waterhouse, A., Winters, A., Comont, D., Scullion, J., Gardias, R., Graee, B. J., Lee, J. A. & Callaghan, T. V. 2012 In : Ambio: A Journal of the Human Environment.41, 3, p. 256-26813 p.
Acclimation and interaction between drought and elevated UV-B in A. thaliana: Differences in response over treatment, recovery and reproductionComont, D. C., Winters, A. L. & Gwynn-Jones, D. 2012 In : Ecology and Evolution.2, 11, p. 2695-270914 p.
Can polyphenol oxidase mitigate photooxidative damage under abiotic stress conditions?Boeckx, T., Kingston-Smith, A., Webster, R., Winters, A., Webb, K. & Gay, A. 2012 p. Abstract No: P1.27
Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus x giganteus using LC-ESI-MS(n)Parveen, I., Threadgill, M. D., Hauck, B. D., Donnison, I. S. & Winters, A. L. 2011 In : Phytochemistry.72, 18, p. 2376-23849 p.
Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus × giganteus using LC–ESI-MSnParveen, I., Threadgill, M. D., Hauck, B. D., Donnison, I. & Winters, A. L. 2011 In : Phytochemistry.72, 18, p. 2376-23849 p.
High-sugar perennial ryegrass as a feed-stock for bioconversion to platform chemicalsWinters, A. L., Leemans, D., Morris, S. M., Pippel, J., Lovatt, A., Charlton, A., Gallagher, J., Smith, L. (ed.), Stafford, A. (ed.) & Weightman, R. (ed.) 2011 In : Aspects of Applied Biology.p. 79-868 p.
Genetic transformation of Miscanthus sinensis with a ferulic acid esterase geneDalton, S. J., Gallagher, J. A., Winters, A. L., Langdon, T., Timms-Taravella, E. & Donnison, I. S. 2011
Can PPO mitigate photooxidative damage under abiotic stress conditions?Boeckx, T., Webster, R., Winters, A. L., Webb, K. J. & Kingston-Smith, A. H. 2011 p. 34
Genetic transformation of Miscanthus sinensis with FAEDalton, S. J., Winters, A. L., Langdon, T., Timms-Taravella, E. & Donnison, I. S. 2011
Characterization of polyphenol oxidase activity in different forage ecotypes with different phenolic substratesCabiddu, A., Lee, M. R. F., Decandia, M., Giovanni, M., Salis, L., Vergiu, M. & Winters, A. L. 2011 p. 543-5431 p.
Isolation, identification and quantification of hydroxycinnamic acid conjugates in leaf and stem tissue of Miscanthus sacchariflorus and sinensis and their potential as platform chemicalsParveen, I., Donnison, I. S., Threadgill, M. D., Hauck, B. D. & Winters, A. L. 2010
Isolation, identification and quantification of hydroxycinnamic acid conjugates in leaf and stem tissue of Miscanthus x giganteus and their potential as platform chemicalsParveen, I., Threadgill, M. D., Hauck, B. D., Donnison, I. S. & Winters, A. L. 2010 p. T5.62
Effect of UV exposure on the abundance of plant phenol compounds in Latuca sativa L. cv. Lolla Rossa leaf tissueWinters, A. L., Holmes, S., Hauck, B. D. & Gwynn-Jones, D. 2010 p. T3.28
Oxidative phenols in forage crops containing polyphenol oxidase enzymesParveen, I., Threadgill, M. D., Moorby, J. M. & Winters, A. L. 2010 In : Journal of Agricultural and Food Chemistry.58, 3, p. 1371-138212 p.
Accumulation of soluble phenolic compounds in the forage grass species, cocksfoot (Dactylis glomerata L).Hauck, B. D., Parveen, I., Gallagher, J. A., Donnison, I. S. & Winters, A. 2010 p. T2.03
Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover) using a large insert BAC libraryWinters, A. L., Heywood, S., Farrar, K., Donnison, I. S., Thomas, A. M. & Webb, K. J. 2009 In : BMC Plant Biology.9, 94
Rapid identification of hydroxycinnamate esters in Miscanthus giganteus and Miscanthus goliath as substrates for polyphenol oxidase using ESI-MS/MSParveen, I., Threadgill, M. D., Hauck, B. D., Donnison, I. S. & Winters, A. L. 2009 In : Planta Medica.75, 9, p. 900
Red clover polyphenol oxidase: activation activity and efficacy under grazingLee, M. R. F., Tweed, J. K. S., Minchin, F. R. & Winters, A. L. 2009 In : Animal Feed Science and Technology.149, 3-4, p. 250-26415 p.
Effect of feeding fresh or conditioned red clover on milk fatty acids and Nitrogen utilisation in lactating dairy cowsLee, M. R. F., Theobald, V. J., Tweed, J. K. S., Winters, A. L. & Scollan, N. D. 2009 In : Journal of Dairy Science.92, 3, p. 1136-114712 p.
Festuca pratensis partial PPO sequenceWinters, A. L. & Gallagher, J. A. 2009
Lolium perenne partial PPO sequenceWinters, A. L. & Gallagher, J. A. 2009
Trifolium pratense PPO1/4Winters, A. L., Webb, K. J. & Donnison, I. S. 2009
Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidaseParveen, I., Winters, A., Threadgill, M. D., Hauck, B. D. & Morris, P. 2008 In : Phytochemistry.69, 16, p. 2799-28068 p.
Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphonel oxidaseParveen, I., Morris, P., Winters, A. L., Hauck, B. D. & Threadgill, M. D. 2008 In : Planta Medica.74, p. T1.512 p.
Polyphenol oxidase activity and protein complexing in damaged red clover herbageLee, M. R. F., Minchin, F. R. & Winters, A. L. 2008 BSAS Annual Conference, Spa Complex, Scarborough, 31 March - 2 April 2008.p. p029
Latent and Active Polyphenol Oxidase (PPO) in Red Clover (Trifolium pratense) and Use of a Low PPO Mutant To Study the Role of PPO in Proteolysis ReductionWinters, A. L., Minchin, F. R., Michaelson-Yeates, T. P. T., Lee, M. R. F. & Morris, P. 2008 In : Journal of Agricultural and Food Chemistry.56, 8, p. 2817-28248 p.
Selective metabolism of glycosidase inhibitors by a specialized moth feeding on Hyacinthoides non-scripta flowersWatson, A. A., Winters, A. L., Corbet, S. A., Tiley, C. & Nash, R. J. 2008 In : Natural Product Communications.3, 1, p. 41-444 p.
Identification of substrates of polyphenol oxidase enzyme from cocksfoot species using NMR and LCMS techniquesParveen, I., Winters, A. L., Hauck, B. D. & Morris, P. 2008
Defence-related polyphenol oxidase gene family resides on an extensive gene cluster in red cloverWinters, A. L., Donnison, I. P., Abberton, M. T., Ravagnani, A. & Webb, K. J. 2008 p. p121
Role of red clover polyphenol oxidase in pathogen resistanceWinters, A. L., Gill, S., Parveen, I., Sullivan, M. L. & Webb, K. J. 2008 p. T3.28
Isolation of an enzyme for the conversion of grass polymeric fructan to fructose, a fermentable substrate for the production of ethanolWinters, A. L., Davies, D. R., Timms-Taravella, E., Jackson, C., Morris, M., Kelly, R., Donnison, I. S. & Gallagher, J. A. 2008
Plant mediated lipolysis of grass genotypes with varying concentrations of polyphenol oxidaseWinters, A. L., Lee, M. R. F., Minchin, F. R., Hopkins, J. J. (ed.), Duncan, A. J. (ed.), McCracken, D. I. (ed.), Peel, S. (ed.) & Tallowin, J. R. B. (ed.) 2007 p. 253-2564 p.
Polyphenols and their influence on gut function and health in ruminants: A reviewTheodorou, M. K., Lee, M. R. F., Winters, A. L., Minchin, F. R., Morris, P., Kingston-Smith, A. H. & MacRae, J. C. 2006 In : Environmental Chemistry Letters.4, 3, p. 121-1266 p.
Polyphenol oxidase activity in grass and its effect on plant-mediated lipolysis and proteolysis of Dactylis glomerata (Cocksfoot) in a simulated rumen environmentLee, M. R. F., Olmos Colmenero, J. D. J., Winters, A. L., Scollan, N. D. & Minchin, F. R. 2006 In : Journal of the Science of Food and Agriculture.86, 10, p. 1503-15119 p.
Biochemistry and molecular biology of polyphenol oxidases in grassesWinters, A. L., Bryant, D. N., Gallagher, J. A., Lee, M. R. F., Minchin, F. R., Parveen, I., Theodorou, M. K. & Morris, P. 2006 p. 303-3042 p.
Modification of the Lowry Assay to measure proteins and phenols in covalently bound complexesWinters, A. L. & Minchin, F. R. 2005 In : Analytical Biochemistry.346, 1, p. 43-486 p.
Substrate-dependent activation of polyphenol oxidase in red cloverMinchin, F. R., Winters, A. L., Morris, P., O'Mara, F. P. (ed.), Wilkins, R. J. (ed.), 't Mannetje, L. (ed.), Lovett, D. K. (ed.), Rogers, P. A. M. (ed.) & Boland, T. M. (ed.) 2005
PPO in red cloverMinchin, F. R., Fothergill, M., Rees, M. E., Winters, A. L., Dalmannsdottir, S. (ed.) & Helgadottir, A. (ed.) 2005 p. 63-675 p.
Effects of manipulating the protein content of white clover on silage qualityWinters, A. L., Minchin, F. R., Davies, Z., Kingston-Smith, A. H., Theodorou, M. K., Griffith, G. W. & Merry, R. J. 2004 In : Animal Feed Science and Technology.116, 3-4, p. 3-42 p.
Plant mediated lipolysis and proteolysis in red clover with different polyphenol oxidase activitiesLee, M. R. F., Winters, A. L., Scollan, N. D., Dewhurst, R. J., Theodorou, M. K. & Minchin, F. R. 2004 In : Journal of the Science of Food and Agriculture.84, 13, p. 1639-16457 p.
Looking glass inhibitors: L-DMDP, a more potent and specific inhibitor of alpha-glucosidases than the enantiomeric natural product DMDPNash, R. J., Asano, N., Winters, A. L., Yu, C. Y., Fleet, G. W. J., Butters, T. D., Ikeda, K., Dwek, R. A., Wormald, M. R. & Wang, M. X. 2004 In : Chemical Communications.p. 1936-19372 p.
The effect of sowing ratio and stage of maturity at harvest on yield, persistency and chemical composition of fresh and ensiled red clover/lucerne bi-cropsMarley, C. L., Winters, A. L., Fraser, M. D., Fychan, A. R. & Jones, R. 2003 In : Grass and Forage Science.58, 4, p. 397-40610 p.
Stereoselective syntheses of 1,4-dideoxy-1,4-imino-octitols and novel tetrahydroxyindolizidinesCarmona, A. T., Fuentes, J. M., Winters, A. L., Vogel, P., Robina, I., Garcia, E. R. & Demange, R. 2003 In : Journal of Organic Chemistry.p. 3874-388310 p.
Intramolecular guanidine epoxide ring opening reactionsDennis, M., Hall, L. M., Murphy, P. J., Thornhill, A. J., Nash, R. J., Winters, A. L., Hursthouse, M. B., Light, M. E. & Horton, P. 2003 In : Tetrahedron Letters.44, 15, p. 3075-30806 p.
Effect of PPO on protein quality in fresh and conserved forageWinters, A. L., Minchin, F. R. & Morris, P. 2003 p. 1-33 p.
Comparison of polyphenol oxidase activity in red clover and perennial ryegrassWinters, A. L., Minchin, F. R., Morris, P. & Merry, R. J. 2003 In : Aspects of Applied Biology.70, p. 15-162 p.
New approaches to forage legume breedingAbberton, M. T., Scollan, N. D., Michaelson-Yeates, T. P. T., Collins, R. P., Minchin, F. L., Winters, A. L., Macduff, J. H. & Marshall, A. H. 2003 Vorträge für Pflanzenzüchtung.p. 22-254 p.
Effect of inoculation with Lactobacillus plantarum on protein degradation during ensilage of red cloverMerry, R. J., Winters, A. L., Leemans, D. K., Lloyd, J. D. & Lowes, K. 2002 Proceedings XIII International Silage Conference. Thomas, C. & Gechie, L. M. (eds.). Scotland's Rural College, p. 11-133 p.
Evaluation of a rapid method for estimating free amino acids in silagesWinters, A. L., Lloyd, J. D., Jones, R. & Merry, R. J. 2002 In : Animal Feed Science and Technology.99, 1-4, p. 177-18711 p.
Development of a novel silage inoculant from small scale laboratory to large clamp silosDavies, D. R., Merry, R. J., Scollan, N. D., Cooper, A., Evans, P. R., Lowes, K., Bakewell, E. L., Leemans, D. K. & Winters, A. L. 2002 Proceedings XIII International Silage Conference. Thomas, C. & Gechie, L. M. (eds.). Scotland's Rural College, p. 11-133 p.
The effect of harvest date and inoculation on the yield, fermentation characteristics and feeding value of kale silageFraser, M. D., Winters, A. L., Fychan, A. R., Davies, D. R. & Jones, R. 2001 In : Grass and Forage Science.56, 2, p. 151-16111 p.
Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performanceWinters, A. L., Fychan, A. R. & Jones, R. 2001 In : Grass and Forage Science.56, 2, p. 181-19212 p.
Conservation of alternative forage cropsJones, R., Theodorou, M. K., Merry, R. J., Adesogan, A. T., Davies, D. R., Dewhurst, R. J., Fraser, M., Fychan, A., Nash, R. J., Kingston-Smith, A., Salawa, M. B., Winters, A., Theobald, V., Roberts, J. E., Evans, S. T., Davies, R. J. T., Leemans, D., Bakewell, E. L., Lloyd, J. D. & Bollard, A. L. 2000 Milk Development Council. (Final report to the Milk Development Council (MDC) - June 2000)
Molecular cloning and expression of a Micromonospora chalcae β-glucosidase encoding gene in Escherichia coliWinters, A., Gallagher, J., Barron, N., Rollan, A. & McHale, A. P. 1996 In : Biotechnology Letters.18, 12, p. 1387-1390
Production of cellulase and beta-glucosidase activity during growth of the actinomycete Micromonospora chalcae on cellulose containing mediaGallagher, J., Winters, A., Barron, N., McHale, L. & McHale, A. P. 1996 In : Biotechnology Letters.18, 5, p. 537-5404 p.
Isolation of a gene expressed during sucrose accumulation in leaves of Lolium temulentum L.Winters, A. L., Gallagher, J., Pollock, C. J. & Parrar, J. F. 1995 In : Journal of Experimental Botany.46, Special Issue, p. 1345-1350
Changes in gene‐expression in response to sucrose accumulation in leaf tissue of Lolium temulentum L.Winters, A. L., Williams, J. H. H., Thomas, D. S. & Pollock, C. 1994 In : New Phytologist.128, 4, p. 591-60010 p.
Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.Hendy, L., Gallagher, J., Winters, A., Hackett, T. J., McHale, L. & McHale, A. P. 1990 In : Biotechnology Letters.12, 9, p. 673-678