Prof John Draper
I currently manage the High Resolution Metabolomics Laboratory and am responsible for oversight of Enabling Technologies within IBERS including core facilities for Metabolomics, Next Generation Sequencing, Plant Phenomics and Bioinformatics.
From a technology perspective I am interested in the development of generic, high throughput phenotyping methodologies, based on global metabolite analysis (metabolomics) for use in a range of fields. My lab is concentrating on metabolite fingerprinting & profiling using high resolution mass spectrometry techniques.
I have established a range of collaborations with laboratories interested in the application of metabolomics technology in food & nutrition research & in plant pathology. We have developed high throughput methods (metabolite fingerprinting, data representation & data analysis) for compositional analysis & comparison of food raw materials. Alongside clinical/veterinary institutions & industry partners, we are currently researching methods for determining dietary exposure & individual responses to diet constituents in humans & domesticated animals from blood & urine analysis.
From a fundamental research perspective my team has pioneered the development of Brachypodium distachyon as a new model system for plant functional genomics. Together with local & international collaborators we played a key role in collecting & characterizing Brachypodium distachyon germplasm in terms of karyotype, comparative genomics, tissue culture behavior, transformation efficiency & pathogen interactions.
Currently I am developing a metabolomics platform for both metabolite identification & high throughput phenotyping in grasses & cereals based on the Brachypodium metabolome. A practical application of this research focuses on a study of metabolic reprogramming in B. distachyon, rice & barley during the biotrophic phases of interaction with rice blast. In collaboration with Nick Talbot (Univ. Exeter, UK) this project extends to an analysis of metabolome changes in Magnaporthae grisae during epidermal cell penetration & colonization.
My research is supported by grants from the BBSRC, The Foods Standards Agency & Industry.
Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets: Plasma lipidome of dog breeds using UHPLC-HRM. Metabolomics 13 152017.
Use of biomarkers to assess fruit and vegetables intake. Proceedings of the Nutrition Society Nutrition Society Summer Meeting, Dublin, United Kingdom of Great Britain and Northern Ireland, 11/07/2016 - 14/07/2016.2017.
Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intake. Molecular Nutrition and Food Research 60 (2) pp. 444-457.2016.
Developing community-based urine sampling methods to facilitate dietary exposure biomarker technology for population assessment. Proceedings of the Nutrition Society 75 (OCE3) E240 Nutrition Society Summer Meeting, Dublin, United Kingdom of Great Britain and Northern Ireland, 11/07/2016 - 14/07/2016.2016.
Quantification of dietary biomarkers in spot urine samples reflects the intake of foods of UK high public health importance. Proceedings of the Nutrition Society 75 (OCE3) E248 Nutrition Society Summer Meeting, Dublin, United Kingdom of Great Britain and Northern Ireland, 11/07/2016 - 14/07/2016.2016.
The food metabolome: A window over dietary exposure. American Journal of Clinical Nutrition 99 (6) pp. 1286-1308. Other2014.
Dietary exposure biomarker-lead discovery based on Metabolomics analysis of urine samples. Proceedings of the Nutrition Society 72 (3) pp. 352-361. Other2013.
Hydroxylated phenylacetamides derived from bioactive benzoxazinoids are bioavailable in humans after habitual consumption of whole grain sourdough rye bread. Molecular Nutrition and Food Research 57 (10) pp. 1859-1873. Other2013.
EPPN: High throughput plant phenotyping facilities at Aberystwyth. EPPN Workshop, Warsaw, Poland, 11/05/2012 - 12/05/2012.2012.
Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples. Metabolomics 7 (4) pp. 469-484. Cadair2011.
Development of high throughput plant phenotyping facilities at Aberystwyth. Phenomics Workshop. Plant and Animal Genome XIX Conference , San Diego, United States of America, 09/01/2011 - 13/01/2011.2011.
Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption. British Journal of Nutrition 106 (6) pp. 812-824. Cadair2011.
Enhancement of plant metabolite fingerprinting by machine learning. Plant Physiology 153 (4) pp. 1506-1520. Cadair2010.
Metabolite fingerprinting of urine suggests breed-specific dietary metabolism differences in domestic dogs. British Journal of Nutrition 103 (8) pp. 1127-1138. Cadair2010.
The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato. Plant Journal 63 (3) pp. 443-457. Cadair2010.
Metabolite signal identification in accurate mass metabolomics data with MZedDB, an interactive m/z annotation tool utilising predicted ionisation behaviour 'rules'. BMC Bioinformatics 10 (227) 227 Cadair Other2009.
Metabolomic analysis reveals a common pattern of metabolic re-programming during invasion of three host plant species by Magnaporthe grisea. Plant Journal 59 (5) pp. 723-737. Cadair2009.
Brachypodium distachyon: making hay with a wild grass. Trends in Plant Science 13 (4) pp. 172-177. Cadair2008.
Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints. Nature Protocols 3 pp. 471-485. Cadair2008.
Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data. Nature Protocols 3 pp. 446-470. Cadair2008.
Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions. Nature Protocols 3 pp. 435-445. Cadair2008.
Detecting a difference - assessing generalisability when modelling metabolome fingerprint data in longer term studies of genetically modified plants. Metabolomics 3 (3) pp. 335-347. Cadair2007.
Representation, comparison, and interpretation of metabolome fingerprint data for total composition analysis and quality trait investigation in potato cultivars. Journal of Agricultural and Food Chemistry 55 (9) pp. 3444-3451. Other Cadair2007.
Soil differentiation using fingerprint Fourier transform infrared spectroscopy, chemometrics and genetic algorithm-based feature selection. Soil Biology and Biochemistry 39 (11) pp. 2888-2896. Cadair2007.
Statistical measures for validating plant genotype similarity assessments following multivariate analysis of metabolome fingerprint data. Metabolomics pp. 349-355. Cadair2007.
Alignment of the genomes of brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization. Genetics 173 (1) pp. 349-362. Other Cadair2006.
On the interpretation of high throughput MS based metabolomics fingerprints with Random Forest. Second International Symposium, CompLife 2006, Cambridge, UK, September 27-29, 2006. Proceedings. International Symposium, CompLife, Cambridge, United Kingdom of Great Britain and Northern Ireland, 27/09/2006 - 29/09/2006. Springer Nature pp. 226-235. Cadair Other2006.
Predicting interpretability of metabolome models based on behavior, putative identity, and biological relevance of explanatory signals. Proceedings of the National Academy of Sciences of the United States of America 103 (40) pp. 14865-14870. Other Cadair2006.
Rapid characterization of microbial biodegradation pathways by FT-IR spectroscopy. Journal of Microbiological Methods 67 (2) pp. 273-280. Cadair2006.
Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoes. Analytical Biochemistry pp. 178-186. Cadair2005.
Hierarchical metabolomics demonstrates substantial compositional similarity between genetically-modified and conventional potato crops. Proceedings of the National Academy of Sciences of the United States of America 102 (40) pp. 14458-14462. Cadair2005.
In planta measurements of oxidative bursts elicited by avirulent and virulent bacterial pathogens suggests that H2O2 is insufficient to elicit cell death in tobacco. Plant, Cell and Environment pp. 548-561. Cadair2005.
Models and meiosis in the ’omics era. In (eds) Recent advances in genetics and breeding of the grasses. Polish Academy of Sciences pp. 97-104.2005.
The use of chemical profiling for monitoring metabolic changes in artificial soil slurries caused by horizontal gene transfer. Metabolomics pp. 305-315. Cadair2005.
A proposed framework for the description of plant metabolomics experiments and their results. Nature Biotechnology 22 (12) pp. 1601-1606. Cadair2004.
Characterization of a proteinase inhibitor from Brachypodium distachyon suggests the conservation of defence signalling pathways between dicotyledonous plants and grasses. Molecular Plant Pathology 5 (4) pp. 267-280. Cadair2004.
Laying the cytotaxonomic foundation of a new model grass, Brachypodium distachyon (L.) Beauv. Chromosome Research 12 (4) pp. 397-403. Cadair2004.
Magnaporthe grisea interactions with the model grass Brachypodium distachyon closely resemble those with rice (Oryza sativa). Molecular Plant Pathology. Wiley pp. 253-265. Cadair2004.
Potential of metabolomics as a functional genomics tool. Trends in Plant Science 9 (9) pp. 418-425. Cadair2004.
Prospects for functional genomics in a new model grass. In (ed) Plant Functional Genomics. Taylor & Francis2004.
Building the molecular cytogenetic infrastructure of a new model grass. In (eds) Application of Novel Cytogenetic and Molecular Techniques in Genetics and Breeding of the Grasses. Polish Academy of Sciences pp. 77-84.2003.
Food quality and microbial succession in ageing earthworm casts: standard microbial indices and metabolic fingerprinting. pp. 5-6. Cadair2003.
Use of earthworm casts to validate FT-IR spectroscopy as a 'sentinel' technology for high-throughput monitoring of global changes in microbial ecology. Pedobiologia 47 (5-6) 7th International Symposium on Earthworm Ecology, Cardiff, United Kingdom of Great Britain and Northern Ireland, 01/09/2001 - 06/09/2002. pp. 440-446. Cadair2003.
Metabolic Engineering, metabolite profiling and machine learning to investigate the phloem-mobile signal in systemic acquired resistance in tobacco. Cadair2002.
Genomic computing. Explanatory analysis of plant expression profiling data using machine learning. Plant Physiology pp. 943-951. Cadair2001.