Dr Leif Skot PhD
I am Principal Investigator in the Breeding Methodologies Group at IBERS with a particular interest in association mapping and genomic selection in forage grass and legume crops. Next generation sequencing and computing technologies provide unprecedented opportunities to enhance crop yield and quality for food and livestock more sustainably through genetic improvement of germplasm. IBERS is the only university department in the UK with active plant breeding programmes, and the objective of my work is to utilise our existing breeding populations and other germplasm as the experimental framework to explore ways of developing and incorporating the genomics information and resources into the recurrent selection based plant breeding programmes for outbreeding forage crops. The aim is to provide a focal point for translational research and the development of new populations to underpin public good plant breeding and to test new genomics based breeding methodologies such as genome wide selection. These methodologies have the added advantage of enabling gene discovery and dissection of complex traits of biological and agronomic importance through association mapping in breeder-relevant germplasm.
- Comparative population genomics of red clover domestication and improvement BBSRC-Industrial Partnership Award with The Genome Analysis Centre and Germinal Holdings (Principal Investigator)
- Genomics-assisted breeding for fatty acid content and composition in perennial ryegrass (Lolium perenne L.) BBSRC LINK1 Award with Germinal Holdings and HCC (Co-Investigator)
- Marker assisted plant breeding on a genomewide scale BBSRC Industrial Partnership award with Germinal Holdings (Principal Investigator)
- Breeding of improved forage varieties to increase protein supply and protein utilisation by ruminants. Technology Strategy Board (Co-Investigator)
- Crop Genetics, Genomics and Germplasm. BBSRC Institute Strategic Programme Grant (Co-PI)
- Colombia and Kenya Partnering Award: Skills sharing for genomic approaches to forage improvement. BBSRC International Partnering Award (Co-PI)
Genomic prediction unifies animal and plant breeding programs to form platforms for biological discovery. Nature Genetics 49 (9) pp. 1297-1303. 10.1038/ng.39202017.
A novel multivariate approach to phenotyping and association mapping of multi-locus gametophytic self-incompatibility reveals S, Z and other loci in a perennial ryegrass (Poaceae) population: Identifying Multi-Locus Gametophytic Self-Incompatibility Genes. Frontiers in Plant Science 8 1331 10.3389/fpls.2017.013312017.
Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement. Scientific Reports 5 17394 10.1038/srep173942015.
Comparitive population genomics of red clover domestication and improvement. Proceedings of the 12th British Grassland Society Research Conference. BGS 12th Research Conference. British Grassland Society pp. 59-60. Cadair2015.
Analysis of changes in population structure over time in components of multi-species swards. In (ed) Grassland Science in Europe: Proceedings of General Meeting of the European Grassland Federation. , Wales pp. 861-863. Cadair2014.
De novo assembly of red clover transcriptome based on RNA-Seq data provides insight into drought response, gene discovery and marker identification. BMC Genomics 15 pp. 453. 10.1186/1471-2164-15-453 Cadair2014.
Genomic Selection In Perennial Ryegrass. Plant and Animal Genome XXI. Other2013.
Temporal changes in population genetic diversity and structure in red and white clover grown in three contrasting environments in northern Europe. Annals of Botany 110 (6) pp. 1341-1350. 10.1093/aob/mcs058 Cadair2012.
Use of translational genomics in legumes to underpin germplasm improvement in red clover. Cadair2011.
Prospects for using genomic selection in plant breeding. Plant Biotech Denmark 2011. Cadair2011.
Allelic variation in the perennial ryegrass FLOWERING LOCUS T gene is associated with changes in flowering time across a range of populations. Plant Physiology 155 (2) pp. 1013-1022. 10.1104/pp.110.169870 Cadair2011.
Lolium perenne partial rs1 gene for putative salt-stress root protein, exons 1-4, strain LTS1. Cadair2011.
AFLP analysis of genetic differentiation in legume germplasm in contrasting environments. In (eds) Grassland Science in Europe. Grassland in a changing world. Proceedings of the 23rd General Meeting of the European Grassland Federation. European Grassland Federation pp. 51-53. Other Cadair2010.
Allelic diversity for candidate genes and association studies: methods and results. In (ed) Sustainable use of Genetic Diversity in Forage and Turf Breeding. Springer Nature pp. 391-396. 10.1007/978-90-481-8706-5_56 Cadair2010.
AFLP analysis of changes in population genetic diversity and structure in forage legume germplasm grown in contrasting environments in Northern Europe. The potential of forage legumes to sustain a high agricultural productivity ? A Nordic perspective. NJF Seminar 432, Hvann eyri, Iceland. Cadair Other2010.
Fine mapping of a major terminal drought tolerance QTL in pearl millet. Cadair2010.
Lolium perenne partial rs1 gene for a putative salt-stress root protein, exons 1-4, strain LTS18. Cadair2009.
Conducting association mapping analysis of global pearl millet germplasm collection to validate candidate genes associated with a major drought tolerance QTL. Plant and Animal Genome XVII Conference. Other Cadair2009.
Molecular basis of response to drought in red clover. Plant Sciences Wales. Cadair2009.
Using gene specific DNA markers to assist targeted manipulation of flowering time and duration and seed yield potential in grass crops. Biotechnology and Biological Sciences Research Council Cadair2009.
Association mapping and exploitation of cross orthologous markers in forage crops. Unknown Publisher Cadair2009.
Understanding the genetic basis of flowering and fertility in the ryegrasses (Lolium spp.). In (eds) Molecular Breeding of Forage and Turf. Proceedings of the 5th International Symposium on the Molecular Breeding of Forage and Turf 2007 (MBFT 2007), Sapporo Convention Center. Springer Nature pp. 185-192. Cadair Other2009.
Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). The XXVII Eucarpia Symposium on Improvement of Fodder Crops and Amenity Grasses. 27th EUCARPIA Symposium on Improvement of Fodder Crops and Amenity Grasses. pp. 86. Cadair Other2008.
Using translational genomics to underpin germplasm improvement for complex traits in crop legumes. 27th EUCARPIA Symposium on Improvement of Fodder Crops and Amenity Grasses. Cadair2008.
Two distinct AFLP types in three populations of marram grass (Ammophila arenaria) in Wales. Plant Genetic Resources: Characterization and Utilization 6 (3) pp. 201-207. 10.1017/S147926210899417X Cadair2008.
Investigating the basis of abiotic stress tolerance in white clover: molecular approaches currently being used in IGER, Aberystwyth. Proceedings of final meeting of COST Action 852. Cadair2008.
Genetically modified hydrographs: integrating grass genetics and soil science. British Society of Soil Science meeting. Cadair2008.
Introgression of crown rust (Puccinia coronata) resistance from meadow fescue (Festuca pratensis) into Italian ryegrass (Lolium multiflorum): Genetic mapping and identification of associated molecular markers. Plant Pathology 55 (1) pp. 62-67. 10.1111/j.1365-3059.2005.01308.x Cadair2006.
Genetics of symbiosis in Lotus japonicus: recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci. Molecular Plant-Microbe Interactions 19 (1) pp. 80-91. 10.1094/MPMI-19-0080 Cadair2006.
Identification of perennial ryegrass (Lolium perenne (L.)) and meadow fescue (Festuca pratensis (Huds.)) candidate orthologous sequences to the rice Hd1(Se1) and barley HvCO1 CONSTANS-like genes through comparative mapping and microsynteny. New Phytologist pp. 239-247. 10.1111/j.1469-8137.2005.01392.x Cadair2005.
Developing technologies for precision breeding. In (eds) Recent Advances in Genetics and Breeding of the Grasses. Polish Academy of Sciences pp. 9-22. Cadair2005.
Differential gene expression in individual papilla-resistant and powdery mildew-infected barley epidermal cells. Molecular Plant-Microbe Interactions 17 (7) pp. 729-738. 10.1094/MPMI.2004.17.7.729 Other Cadair2004.
Synteny between a major heading-date QTL in perennial ryegrass (Lolium perenne L.) and the Hd3 heading-date locus in rice. Theoretical and Applied Genetics 108 (5) pp. 822-828. 10.1007/s00122-003-1495-6 Cadair2004.
The application of genecology to the discovery of associations between phenotype and molecular markers in natural populations of perennial ryegrass. International Lolium Genome Initiative. Plant and Animal Genome XII Conference. Cadair Other2004.
Genetic variation in natural populations of perennial ryegrass in relation to flowering time and soluble carbohydrates. Proceedings of the 17th Eucarpia General Congress, Tulln, Austria, 6-11 September 2004. 17th Eucarpia General Congress. pp. 6-11. Cadair Other2004.
Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro. In (eds) Genetic Variation for Plant Breeding. Proceedings of the 17th Eucarpial General Congress. University of Natural Resources and Life Sciences, Vienna pp. 279-283. Cadair Other2004.
Association mapping of molecular markers for flowering time using natural populations of Lolium perenne. Proceedings of the 7th British Grassland Society (BGS) Research Conference, Aberystwyth, 1-3 September 2003. 7th British Grassland Society Research Conference. pp. 75-76. Cadair Other2003.
Marker assisted breeding to enhance forage quality in ryegrass varieties. Aspects of Applied Biology: Proceedings 'Crop Quality' Its role in sustainable livestock production. Assocation of Applied Biologists Conference. Association of Applied Biologists pp. 183-186. Cadair Other2003.
The use of genetic markers in grass breeding. Proceedings 25th EUCARPIA Fodder Crops and Amenity Grasses Section Meeting. Czech Journal of Genetics and Plant Breeding, vol. 39 Proceedings 25th EUCARPIA Fodder Crops and Amenity Grasses Section Meeting. , Switzerland pp. 112-119. Cadair2003.
Approaches for associating molecular polymorphisms with phenotypic traits based on linkage disequilibrium in natural populations of Lolium perenne. In (ed) Molecular Breeding for the Genetic Improvement of Forage Crops and Turf. The 4th international symposium on the molecular breeding of forage and turf, a satellite workshop of the XXth International Grassland Congress. Wageningen Academic Publishers pp. 978-90. Cadair Other2001.
Biological interactions in grassland soils and productivity. In (eds) Grassland Ecosystems: An Outlook into the 21st Century. Proceedings 19th International Grassland Congress. Fundação de Estudos Agrarios Luiz de Queiroz pp. 167-168. Cadair2001.
A secondary effect of transformation in Rhizobium leguminosarum transgenic for Bacillus thuringiensis subspecies tenebrionis δ-endotoxin ( cryIIIA ) genes. Part 2. Theoretical and Applied Genetics 100 (5) pp. 820-823. 10.1007/s001220051357 Cadair2000.
Application of biotechnology to Lotus breeding. In (ed) Trefoil: the Science and Technology of the Lotus. Cssa Special Publication Crop Science Society of America pp. 199-228. Cadair1999.
Over-expression of sucrose phosphate synthase in Arabidopsis thaliana results in increased foliar sucrose/starch ratios and favours decreased foliar carbohydrate accumulation in plants after prolonged growth with CO2 enrichment. Journal of Experimental Botany 49 (321) pp. 669-680. 10.1093/jxb/49.321.669 Cadair1998.
Introducing and Expressing Genes in Legumes. In (eds) Recombinant proteins from Plants: Production and Isolation of Clinically Useful Compounds. Methods in Microbiology Springer Nature pp. 27-38. Cadair1998.
A secondary effect of transformation in Rhizobium leguminosarum transgenic for Bacillus thuringiensis subspecies tenebrionis ä-endotoxin (cryIIIA) genes. Theoretical and Applied Genetics 95 (7) pp. 1062-1068. 10.1007/s001220050663 Cadair1997.
Down-regulation of sucrose synthase expression and activity in transgenic hairy roots of Lotus japonicus. Symbiosis 22 (3) pp. 241-254. Cadair1997.
Inheritance of a transgene in Lotus corniculatus L. In (ed) Seeds of Progress: Proceedings of the BGS/BSPB/NIAB/SAC Conference. Occasional symposium Proceedings of the BGS/BSPB/NIAB/SAC Conference. British Grassland Society pp. 84-85. Cadair1997.
Carbon and nitrogen metabolism in plant-derived ineffective nodules of pea (Pisum sativum L.). In (eds) Biological Fixation of Nitrogen for Ecology and Sustainable Agriculture. NATO ASI Series / Ecological Sciences Springer Nature pp. 227-230. Cadair1997.
Genetic transformation of Lotus corniculatus using disarmed Agrobacterium tumefaciens and the inheritance of transgenes in the T1 generation. Transgenic Research 5 (5) pp. 303-312. 10.1007/BF01968940 Cadair1996.
A study of Rhizobium leguminosarum biovar trifolii populations from soil extracts using randomly amplified polymorphic DNA fragment patterns. Canadian Journal of Microbiology 41 (4-5) pp. 336-344. 10.1139/m95-046 Cadair1995.
Anatomy, physiology and biochemistry of root nodules of Sprint-2 Fix-, a symbiotically defective mutant of pea (Pisum sativum L.). Journal of Experimental Botany 46 (12) pp. 1809-1816. 10.1093/jxb/46.12.1809 Cadair1995.
Introduction of genes to rhizobia. In (eds) Manual for Methods on Soil Microbiology for Agricultural Purposes. Brazilian Agricultural Research Corporation pp. 201-226. Cadair1995.
RFLP and QTL analysis of seedling thermotolerance in contrasting genotypes of pearl millet. In (eds) ODA Plant Series Research Compendium 1993. pp. 39-40. Cadair1994.
Expression of insecticidal activity in Rhizobium containing the delta-endotoxin gene cloned from Bacillus thuringiensis subsp. tenebrionis. Plant and Soil 127 (2) pp. 285-295. 10.1007/BF00014436 Cadair1990.
Direct evidence for changes in the resistance of the legume root nodules to O2 diffusion. Journal of Experimental Botany 38 (192) pp. 1129-1140. Cadair1987.
Nitrogen fixation and oxygen in legume root nodules. In (ed) Oxford Surveys of Plant Molecular and Cell Biology. Oxford University Press pp. 275-314. Cadair1986.