Module Identifier | BS23720 | ||||||||||||||
Module Title | APPLIED MOLECULAR BIOLOGY | ||||||||||||||
Academic Year | 2007/2008 | ||||||||||||||
Co-ordinator | Professor John Draper | ||||||||||||||
Semester | Semester 1 | ||||||||||||||
Other staff | Dr Glyn Jenkins, Dr Luis A J Mur, Dr Ian M Scott | ||||||||||||||
Course delivery | Lecture | 30 x 1h lectures | |||||||||||||
Other | 3 Hours. 3 X 3-HOUR WORKSHOPS; 1 X 2-HOUR WORKSHOP | ||||||||||||||
Assessment |
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1) Fundamental molecular biological processes
i) Enzymology of DNA replication
ii) DNA repair
iii) Gene structure and RNA synthesis in prokaryotes
iv) mRNA processing in eukaryotes
v) Control of transcription and translation
2) Analysing DNA, transcripts and proteins
i) Polymerase chain reaction
ii) Restriction enzymes and DNA modification enzymes
iii) DNA sequencing
iv) Gene foot-printing technologies to define DNA/protein interactions
v) Techniques for measuring transcript levels
vi) Protein analysis
3) Gene library, construction, screening and DNA sequencing
i) DNA cloning vectors and methodologies
ii) Generating copy DNA and cDNA libraries
iii) Bacteriophage cloning and genomic libraries
iv) Gene fusion and expression vectors
4) Genomics
i) Introduction to genomics
ii) Genome sequencing strategies
iii) Model organisms
iv) Comparative genomics
v) Study of chromosomes, FISH, GISH
5) Transgenic technology and example applications
i) Gene transfer vectors for plants
ii) Generation of transgenic plants using tissue culture
ii) Concept of marker genes and reporter gene technologies
iii) Transgenic plants in agriculture
iv) Generation of transgenic animals
v) Gene therapy
6) Workshops
The practical component of the module will focus on workshops aiming to provide experience in design of PCR experiments, DNA mapping by restriction digestion and examination of DNA sequence to evaluate gene structure. There will be some opportunity to use tissue culture to generate transgenic plant cells and investigate the activity of reporter genes.
This module is at CQFW Level 5