Protocol: 
DC title: Library reformatting from Master tubes to Daughter plates
DC subject: drug screening
DC author: the Computational Biology group
Organization: UWA
DC submission data: December 2007

Goal: To make 1 working copy of the chemical compound library. i.e. 1 x 96 tube rack to 1 x 96 well daughter plate.

Procedure of preparation for experiment:

Equipment setup action: 
Equipment: automated dry store
Goal: to leave enough space for the daughter plates



Experiment action: Check:
Pre-condition: plate is in automated dry store 
Pre-condition: master tube rack is in offline dry store
Pre-condition: 96 master tubes have 10mM compound in 100% DMSO 


Experiment action: Assign name:
Object: plate
New name: daughter plate

Experiment action: Move:
Object: master tube rack
Equipment: manually
Start location: in offline dry store 
End location: in automated dry store


Experiment action: Move:
Object: daughter plate
Start location: in automated dry store
End location: in transfer station

Experiment action: Move:
Object: daughter plate
Equipment: main arm
Start location: in transfer station
End location: in liquid handler platform

Experiment action: Move:
Object: master tube rack
Equipment: arm
Start location: in automated dry store
End location: in decapper

Experiment action: De-lid:
Equipment: Tube rack decapper
Top: cap
Base: master tube

Experiment action: De-lid:
Equipment: liquid handler
Top: plate lid
Base: daughter plate
Lid location: in liquid handler platform

Experiment action: Move:
Object: master tube rack
Equipment: arm
Start location: in decapper 
End location: in liquid handler platform

Experiment action: Aspirate:
Component: single aliquot
Container: master tube
Volume: 20 uL

Experiment action: Add:
Component: single aliquot
Volume: 20 uL
Container: daughter plate wells

Experiment action: Move:
Object: master tube rack
Equipment: arm
Start location: in liquid handler platform
End location: in decapper

Experiment action: Cover:
Equipment: decapper
Top: purge
Base: master tube

Experiment action: Move:
Object: master tube rack
Equipment: arm
Start location: in decapper
End location: in automated dry store

Experiment action: Cover
Equipment: liquid handler
Top: plate lid
Base: daughter plate
Lid location: in liquid handler platform


Experiment action: Move:
Object: daughter plate
Equipment: arm
Start location: in liquid handler platform
End location: in automated dry store

Experiment action: Move:
Object: master tube rack
Equipment: manually
Start location: in automated dry store
End location: in offline dry store 

Experiment action: Check:
Post-condition: 96 daughter plate wells have 10mM compound in 100% DMSO 
Post-condition: daughter plate is in automated dry store
Post-condition: master tube rack is in offline dry store

------------------------------------------------------------

Equipment:
plates: 96 well, acoustic ready COC, lidded
master tubes: rack; to store compound library at 10mM 
Offline dry store   1
Automated dry store 1
Tube rack decapper  1
Liquid handler with gripper and 96 head     1
Arm                 1


Object:
daughter plate
master tube rack

Location:
In transfer station 
On liquid handler platform
In offline dry store 
In automated dry store 
In decapper