Module Identifier BS22520  
Academic Year 2000/2001  
Co-ordinator Dr Gerard Bishop  
Semester Semester 1  
Other staff Dr Glyn Jenkins, Dr Mustak Kaderbhai, Dr David Lamb  
Pre-Requisite BS10510 Introductory Genetics, BS10910 Introductory Biochemistry  
Course delivery Lecture   30 Hours  
  Workshop   6 Hours (3 x 2 hours)  
Assessment Exam   3 Hours One 3-hour theory paper   70%  
  Practical exercise   Continuous assessment associated with workshops   30%  
  Resit assessment   3 Hours One 3-hour theory paper (plus resubmission of failed coursework or an alternative)    

Aims and objectives
The module is aimed at providing the background knowledge and understanding of molecular approaches in tackling biological problems.

This is a core module for those wishing use molecular approaches in biology.
Initially the module will focus on the molecular basics of DNA replication, DNA transcription and RNA translation.
Detailed description of DNA replication, the enzymes involved and how replication proceeds will be presented.
Enzymes and mechanisms involved in the transcription of both eukaryotic and prokaryotic genes will be described.
The components and machinery of RNA translation will be presented.
A basic understanding of the lac operon will also be explained.
The concepts of gene cloning and recombinant gene expression will be presented.
Uses of Plasmid vectors, antibiotic resistance and restriction endo-nucleases will be explained
Choice of cloning vector for the size of DNA to be cloned and organism for gene cloning will be explained.
Techniques for making and screening recombinant DNA libraries will be explained.
Including, agarose gel electrophoresis, DNA-gel blot analysis (Southerns).
Essentials of Polymerase Chain Reaction (PCR) techniques will be taught.
Techniques for studying DNA, including DNA sequencing, will be presented.
Techniques for studying RNA, including RNA-gel blot analysis and quantitative RT-PCR will be taught.
Techniques for studying proteins, Western blots, 2-D gels, elisa techniques will be presented.
More sophisticated techniques of in situ hybridisation will be presented.
Molecular biology of selected organisms will be presented namely Yeast, plants and mice. Including how transgenics of these organisms can be made.

Learning outcomes
On completion of the module the student will

Reading Lists
** Recommended Text
Glick, B.R. & Pasternak, J.J.. (1998) Molecular biotechnology. 2nd. ASM Press.
Lewin, B.. (2000) Genes VII. Oxford Unviersity Press
Lodish,H., Berk, A., Zipursky, S.L., Matsudaira, P., Baltimore, D., Darnell, J.,. (2000) Molecular cell biology. 4th. W.H. Freeman & Company
Turner, P.C., McLennan, A.G., Bateas, A.D. & White, M.R.H.. (2000) Instant notes Molecular Biology. 2nd. BIOS Scientific Publishers Ltd