|| BS22520 |
|| MOLECULAR BIOLOGY |
|| 2003/2004 |
|| Dr Gerard J Bishop |
|| Semester 1 |
|| Dr Glyn Jenkins, Dr Mustak A Kaderbhai, Dr Michael K Winson |
|| BS10510 , BS10910 |
| Course delivery
|| Lecture || 30 Hours |
|| Practical || 11 Hours (3 x 3 hour workshops) (1 x 2 hour workshop) |
|Assessment Type||Assessment Length/Details||Proportion|
|Semester Exam||3 Hours One 3-hour theory paper ||70%|
|Semester Assessment|| Practical Exercise: Continuous assessment of practical work ||30%|
|Supplementary Assessment||3 Hours One 3-hour theory paper (plus resubmission of failed coursework or an alternative) || |
On completion of the module the student will
have knowledge of DNA replication, DNA transcription and RNA translation
have a grounding in the key concepts of molecular approaches to study biological problems
have knowledge of techniques used in molecular biology
be aware of applications of molecular biology including how transgenic organisms can be made.
This module is aimed at providing the fundamental knowledge and key concepts of molecular approaches used in tackling biological problems.
This is a core module for those wishing to use molecular approaches in biology.
Initially the module will focus on the molecular basics of DNA replication, DNA transcription and RNA translation.
Detailed description of DNA replication, the enzymes involved and how replication proceeds will be presented.
Enzymes and mechanisms involved in the transcription of both eukaryotic and prokaryotic genes will be described.
The components and machinery of RNA translation will be explained.
A basic understanding of the lac operon will also be presented.
The concepts of gene cloning and recombinant gene expression will be presented.
Uses of Plasmid vectors, antibiotic resistance and restriction endo-nucleases will be explained
Factors affecting choice of cloning vectors will be explained, techniques for making and screening recombinant DNA libraries will be discussed, including agarose-gel electrophoresis and DNA gel blot analysis (Southerns).
Essentials of Polymerase Chain Reaction (PCR) techniques will be taught.
Techniques for studying DNA, including DNA sequencing will be presented.
Techniques for studying RNA, including RNA-gel blot analysis and quantitative RT-PCR will be taught.
Techniques for studying proteins, Western blots, 2-D gels, ELISA techniques will be presented.
More sophisticated techniques of in situ hybridisation will be presented.
An introduction for Genomics and Bioinformatics will be given.
Molecular biology of selected organisms will be presented, including how transgenics of these organisms can be made.
** Recommended Text
Lewin, B. (2000) Genes VII
Oxford Unviersity Press
Lodish,H., Berk, A., Zipursky, S.L., Matsudaira, P., Baltimore, D., Darnell, J., (2000) Molecular cell biology
4th. W.H. Freeman & Company
Turner, P.C., McLennan, A.G., Bates, A.D., & White, M.R.H. (2000) Instant notes molecular biology
2nd. BIOS Scientific Publishers Ltd.
Brown, T.A. (2002) Genomes II
BIOS Scientific Publishers Ltd.
This module is at CQFW Level 5