Synthetic Defined (SD) / Minimal Medium


This medium is considered to contain only what is essential for yeast growth.
A yeast strain is considered auxotrophic if it can no longer grow on a defined
medium that the wild type can grow on. This medium was used in all auxotrophic
growth experiments.

The medium was prepared using milli-Q grade water. It was prepared in screw
bottles and autoclaved at 121 degreesC, and 15psi for 15 minutes.

Contents:
yeast nitrogen base (without amino acids), 6.7g/L
Dextrose/D-glucose (2.5%)
Supplemented with the auxotrophic requirements (e.g Met, Leu, Ura, His).
G-418 sulphate (200ug/L, provided yeast strain is geneticin resistant)