Dr Manfred Beckmann PhD
Dr Beckmann obtained his doctorate in analytical chemistry at the C.v.O. University Oldenburg, Germany, on the identification of pheromones in marine polychaetes. Since then he has contributed as a marine chemical ecologist to the understanding of chemical communication and antifouling (HKUST, Hongkong), to measure and estimate production of green house gases in wetlands (Cardiff University) and to the investigatigation of metabolic changes in GM-crops, during host-pathogen interactions and dietary effects on mammalian biofluids (Aberystwyth).
He is now a research lecturer and Metabolomics Champion in the High Resolution Metabolomics Laboratory at Aberystwyth University. His research interests are in high through-put ultra-high resolution Metabolomics of difficult matrices and 'real world samples'.
Current research activities focus on Metabolomics: the non-targeted and comprehensive analysis of all metabolites in biological material detecting unforeseen metabolic changes.
Metabolomics is the sum of a well designed and performed experiment, sampling strategies, sample preparation, instrumental analysis, raw data conversion, signal processing, data analysis, and data interpretation.
The outcome of a Metabolomics experiment and interpretability of data very much depend on all members of a skilled team and their tools. In order to minimize sources of variability and bias, robust methods and protocols have been developed in a team effort orchestrated by Prof. John Draper at IBERS over the last 15 years, and are the subject of future activities.
As an analytical chemist I am interested in maximising the information content of Metabolomics data through signal processing and data mining, metabolite identification and characterisation of unknown signals. I am responsible for the functionality of the IBERS High Resolution Metabolomics Laboratory and concerned with method development and optimisation of the following Metabolomics techniques:
- High through-put ultra-high resolution Flow Infusion Electrospray Mass Spectrometry (FIE-MS)
- High through-put ultra-high resolution LC-MS
- High through-put GC-tof-MS
- Metabolite targeted analysis and quantification.
I am particularly interested in applying Metabolomics methodologies to study the effect of diet on urinary and plasma metabolome and resulting changes in physiology. In collaboration with clinical/veterinary institutions in academia and industry, we are currently determining dietary exposure and individual responses to diet constituents in humans and domesticated animals. A key mission is the search for biomarkers of 'health and wellbeing'.
Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line. Journal of Ethnopharmacology 10.1016/j.jep.2017.08.0062017.
Diversity and association of phenotypic and metabolomic traits in the close model grasses Brachypodium distachyon, B. stacei and B. hybridum. Annals of Botany 119 (4) pp. 545-561. 10.1093/aob/mcw239 Cadair2017.
Objective assessment of dietary patterns by use of metabolic phenotyping: a randomised, controlled, crossover trial. Lancet Diabetes and Endocrinology 5 (3) pp. 184-195. 10.1016/S2213-8587(16)30419-3 Cadair2017.
Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets: Plasma lipidome of dog breeds using UHPLC-HRM. Metabolomics 13 15 10.1007/s11306-016-1152-0 Cadair2017.
Developing community-based urine sampling methods to facilitate dietary exposure biomarker technology for population assessment. Proceedings of the Nutrition Society 75 (OCE3) E240 Nutrition Society Summer Meeting. 10.1017/S002966511600255X Cadair2016.
Quantification of dietary biomarkers in spot urine samples reflects the intake of foods of UK high public health importance. Proceedings of the Nutrition Society 75 (OCE3) E248 Nutrition Society Summer Meeting. 10.1017/S0029665116002639 Cadair2016.
An Analytical Pipeline for Quantitative Characterization of Dietary Intake: Application To Assess Grape Intake. Journal of Agricultural and Food Chemistry 64 (11) pp. 2423-2431. 10.1021/acs.jafc.5b05878 Cadair2016.
Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intake. Molecular Nutrition and Food Research 60 (2) pp. 444-457. 10.1002/mnfr.201500495 Cadair2016.
Dietary compliance in a human intervention study investigating the impact of specific foods on urinary metabolites. Proceedings of the Nutrition Society 74 (OCE1) E127 10.1017/S00296651150014212015.
No effect of oral tyrosine administration on mood and motivation or heart rate variability during endurance performance in the heat. 19th Annual Congress of the European College of Sport Science. Cadair2015.
Acute oral tyrosine supplementation does not prevent the decline in maximal handgrip force in hyperthermic subjects. 19th Annual Congress of the European College of Sport Science. Cadair2014.
Hydroxylated phenylacetamides derived from bioactive benzoxazinoids are bioavailable in humans after habitual consumption of whole grain sourdough rye bread. Molecular Nutrition and Food Research 57 (10) pp. 1859-1873. 10.1002/mnfr.201200777 Cadair2013.
Acute oral administration of a tyrosine and phenylalanine-free amino acid mixture reduces exercise capacity in the heat. European Journal of Applied Physiology European Journal of Applied Physiology and Occupational Physiology 113 (6) pp. 1511-1522. 10.1007/s00421-012-2577-4 Cadair2013.
Flow infusion electrospray ionisation mass spectrometry for high throughput, non-targeted metabolite fingerprinting: A Review. Metabolomics 9 (1 Supplement) pp. S4–S29. 10.1007/s11306-012-0449-x Cadair2013.
Deciphering systemic wound responses of the pumpkin extrafascicular phloem by metabolomics and stable isotope-coded protein labeling (ICPL). Plant Physiology 160 (4) pp. 2285-99. 10.1104/pp.112.205336 Cadair2012.
Fourier transform ion cyclotron resonance mass spectrometry for plant metabolite profiling and metabolite identification. Methods in Molecular Biology. Springer Nature pp. 157-176. 10.1007/978-1-61779-594-7_11 Cadair2012.
Effects of changing rumen protozoa populations in sheep on plasma metabolomic profiles. 8th INRA-RRI International Symposium on Gut Microbiology, Clermont Ferrand, France. Cadair2012.
Acute oral tyrosine administration does not improve exercise performance in the heat in man. The Biomedical Basis of Elite Performance. Cadair2012.
Oral tyrosine supplementation improves exercise capacity in the heat. European Journal of Applied Physiology European Journal of Applied Physiology and Occupational Physiology 111 (12) pp. 2941-2950. 10.1007/s00421-011-1921-4 Cadair2011.
Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption. British Journal of Nutrition 106 (6) pp. 812-824. 10.1017/S0007114511001164 Cadair2011.
A tyrosine and phenylalanine-free amino acid mixture decreases exercise capacity in the heat. The British Association of Sport and Exercise Sciences Conference 2011. Cadair2011.
Oral tyrosine supplementation improves exercise capacity in the heat. BASES 2010 Annual Student Conference. Cadair2010.
Metabolomic analysis reveals a common pattern of metabolic re-programming during invasion of three host plant species by Magnaporthe grisea. Plant Journal 59 (5) pp. 723-737. 10.1111/j.1365-313X.2009.03912.x Cadair2009.
Metabolite signal identification in accurate mass metabolomics data with MZedDB, an interactive m/z annotation tool utilising predicted ionisation behaviour 'rules'. BMC Bioinformatics 10 (227) 227 10.1186/1471-2105-10-227 Cadair Other2009.
Oral tyrosine supplementation improves exercise capacity in the heat. 6th Wales Institute of Sport, Health and Exercise Science Conference. Cadair2009.
Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data. Nature Protocols 3 pp. 446-470. 10.1038/nprot.2007.511 Cadair2008.
Detecting a difference - assessing generalisability when modelling metabolome fingerprint data in longer term studies of genetically modified plants. Metabolomics 3 (3) pp. 335-347. 10.1007/s11306-007-0064-4 Cadair2007.
Representation, comparison, and interpretation of metabolome fingerprint data for total composition analysis and quality trait investigation in potato cultivars. Journal of Agricultural and Food Chemistry 55 (9) pp. 3444-3451. 10.1021/jf0701842 Other Cadair2007.
Predicting interpretability of metabolome models based on behavior, putative identity, and biological relevance of explanatory signals. Proceedings of the National Academy of Sciences of the United States of America 103 (40) pp. 14865-14870. 10.1073/pnas.0605152103 Other Cadair2006.
On the interpretation of high throughput MS based metabolomics fingerprints with Random Forest. Second International Symposium, CompLife 2006, Cambridge, UK, September 27-29, 2006. Proceedings. International Symposium, CompLife. Springer Nature pp. 226-235. 10.1007/11875741_22 Cadair Other2006.
Hierarchical metabolomics demonstrates substantial compositional similarity between genetically-modified and conventional potato crops. Proceedings of the National Academy of Sciences of the United States of America 102 (40) pp. 14458-14462. 10.1073/pnas.0503955102 Cadair2005.
Metabolic Engineering, metabolite profiling and machine learning to investigate the phloem-mobile signal in systemic acquired resistance in tobacco. Cadair2002.