Dr David Whitworth
Office: Room S22, Cledwyn Building, Penglais
Phone: +44 (0) 1970 621828
- Degree scheme co-ordinator for C700 Biochemistry
Signalling pathways in the social predator Myxococcus xanthus: the dynamics and specificity of protein-protein interactions.
- Nutrient acquisition - (regulation of phosphate metabolism)
- Responses to stress - (regulation of carotenoid synthesis, multicellular development)
- Protein-protein interactions - (signalling pathway connectivity - experimental and computational)
- Dynamic systems - (network properties, evolution and population behaviour/dynamics)
- Bioinformatics - (comparative genomics, evolution of signalling networks)
- Structural bioinformatics - (structure prediction, interaction modelling)
My research focuses on bacterial signalling pathways and how they alter behaviour in response to a changing environment. In particular, I am interested in the social and predatory myxobacteria, and how they use two-component signalling pathways to co-ordinate group behaviour. Experimental work is investigating the protein-protein interactions between TCS transmitter and receiver domains, which define the structure of TCS pathways. As an extension of this work, we are also characterising the regulation of phosphate assimilation and its inter-relation with multicellular fruiting body formation, a regulon that involves at least seven TCS proteins. Benchwork is being complemented by theoretical studies. One such study uses comparative genomics to probe the evolution of TCSs, and has provided a method for predicting transmitter-receiver domain partnerships, many of which are being confirmed experimentally.
Can the bacterial community of a High Arctic glacier surface escape viral control? Frontiers in Microbiology 7 956 10.3389/fmicb.2016.009562016. (In press)
MetaPred2CS: a sequence-based meta-predictor for protein-protein interactions of prokaryotic two-component system proteins. Bioinformatics 10.1093/bioinformatics/btw4032016.
Recasting a traditional laboratory practical as a "Design-your-own protocol" to teach a universal research skill. Biochemistry and Molecular Biology Education 10.1002/bmb.209482016.
An off-the-shelf, authentic and versatile undergraduate molecular biology practical course. Biochemistry and Molecular Biology Education 43 (6) pp. 434-440. 10.1002/bmb.208932015.
Composition of distinct sub-proteomes in Myxococcus xanthus: metabolic cost and amino acid availability. Amino Acids 47 (12) pp. 2521-2531. 10.1007/s00726-015-2042-x2015.
Genome-wide analysis of myxobacterial two-component systems: genome relatedness and evolutionary changes. BMC Genomics 16 (1) 780 10.1186/s12864-015-2018-y2015.
Genome-wide prediction of prokaryotic two-component system networks using a sequence-based meta-predictor. BMC Bioinformatics 16 (1) 297 10.1186/s12859-015-0741-72015.
P2CS: updates of the prokaryotic two-component systems database. Nucleic Acids Research 43 ((D1)) pp. D536-D541. 10.1093/nar/gku9682015.
Synergism between bacterial GAPDH and OMVs: disparate mechanisms but co-operative action. Frontiers in Microbiology 6 1231 10.3389/fmicb.2015.012312015.
Online assessment of learning and engagement in university laboratory practicals. British Journal of Educational Technology 10.1111/bjet.121932014.
The fist bump: a more hygienic alternative to the handshake. American Journal of Infection Control 42 (8) pp. 916-917. 10.1016/j.ajic.2014.04.0112014.
P2RP: a Web-based framework for the identification and analysis of regulatory proteins in prokaryotic genomes. BMC Genomics 14 (1) pp. 269. 10.1186/1471-2164-14-2692013.
P2TF: a comprehensive resource for analysis of prokaryotic transcription factors. BMC Genomics 13 628 10.1186/1471-2164-13-6282012.
Predatory activity of Myxococcus xanthus outer-membrane vesicles and properties of their hydrolase cargo. Microbiology 158 (Pt 11) pp. 2742-2752. 10.1099/mic.0.060343-02012.
Myxobacterial Vesicles: Death at a Distance? Advances in Applied Microbiology 75 pp. 1-31. 10.1016/B978-0-12-387046-9.00001-32011.
Correlations between the role, sequence conservation, genomic location and severity of phenotype in myxobacterial developmental genes. FEMS Microbiology Letters 312 (1) pp. 40-45. 10.1111/j.1574-6968.2010.02092.x Cadair2010.
P2CS: a database of prokaryotic two-component systems. Nucleic Acids Research 39 (1) pp. D771-D776. 10.1093/nar/gkq10232010.
Life with little oxygen: lessons from microbes. IBERS Knowledge-based Innovations pp. 19-22. Other2009.
Phosphate acquisition components of the Myxococcus xanthus Pho regulon are regulated by both phosphate availability and development. Journal of Bacteriology 190 (6) pp. 1997-2003. 10.1128/JB.01781-07 Cadair2008.
Complete genome sequence of the myxobacterium Sorangium cellulosum. Nature Biotechnology 25 (11) pp. 1281-1289. 10.1038/nbt13542007.
Evolution of gene overlaps: relative reading frame bias in prokaryotic two-component system genes. Journal of Molecular Evolution 64 (4) pp. 457-462. 10.1007/s00239-006-0180-12007.
Myxobacteria: Multicellularity and Differentiation. 1stth edn, ASM Press2007.
Two-Component Signal Transduction System of the Myxobacteria. In D. Whitworth (ed), Myxobacteria: Multicellularity and Differentiation. ASM Press pp. 169-189.2007.
The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus. Microbiology 152 (6) pp. 1609-1620. 10.1099/mic.0.28820-02006.
Four Unusual Two-Component Signal Transduction Homologs, RedC to RedF, Are Necessary for Timely Development in Myxococcus xanthus. Journal of Bacteriology 187 (23) pp. 8191-8195. 10.1128/JB.187.23.8191-8195.2005 Other Cadair2005.
Light-induced carotenogenesis in Myxococcus xanthus functional characterization of the ECF sigma factor CarQ and antisigma factor CarR. Molecular Microbiology 48 (1) pp. 237-251. 10.1046/j.1365-2958.2003.03431.x2003.