Photograph of Dr David Whitworth.Dr David Whitworth

Senior Lecturer


Office: Room S22, Cledwyn Building, Penglais
Phone: +44 (0) 1970 621828


Teaching Areas

Semester 1:

BR12410 Study and Communication Skills
BR23210 Proteins and Enzymes
BR32110 Data-handling in biochemistry
BR32300 Dissertation

Semester 2:

BR12010 Metabolism
BR12210 Molecular Lab Skills
BR22920 Practical Skills in Biochemistry
BR24510 Biochemistry in Practice
BR32330 Dissertation


Signalling pathways in the social predator Myxococcus xanthus: the dynamics and specificity of protein-protein interactions.

  • Nutrient acquisition - (regulation of phosphate metabolism)
  • Responses to stress - (regulation of carotenoid synthesis, multicellular development)
  • Protein-protein interactions - (signalling pathway connectivity - experimental and computational)
  • Dynamic systems - (network properties, evolution and population behaviour/dynamics)
  • Bioinformatics - (comparative genomics, evolution of signalling networks)
  • Structural bioinformatics - (structure prediction, interaction modelling)  

My research focuses on bacterial signalling pathways and how they alter behaviour in response to a changing environment. In particular, I am interested in the social and predatory myxobacteria, and how they use two-component signalling pathways to co-ordinate group behaviour. Experimental work is investigating the protein-protein interactions between TCS transmitter and receiver domains, which define the structure of TCS pathways. As an extension of this work, we are also characterising the regulation of phosphate assimilation and its inter-relation with multicellular fruiting body formation, a regulon that involves at least seven TCS proteins. Benchwork is being complemented by theoretical studies. One such study uses comparative genomics to probe the evolution of TCSs, and has provided a method for predicting transmitter-receiver domain partnerships, many of which are being confirmed experimentally.



Edwards Rassner, S., Anesio, A., Girdwood, S.E., Hell, K., Gokul, J., Whitworth, D., Edwards, A. 2016. (In press) Can the bacterial community of a High Arctic glacier surface escape viral control? Frontiers in Microbiology 7 956 10.3389/fmicb.2016.00956

Kara, A., Vickers, M., Swain, M., Whitworth, D., Fernandez-Fuentes, N. 2016. MetaPred2CS: a sequence-based meta-predictor for protein-protein interactions of prokaryotic two-component system proteins. Bioinformatics 10.1093/bioinformatics/btw403

Whitworth, D.E. 2016. Recasting a traditional laboratory practical as a "Design-your-own protocol" to teach a universal research skill. Biochemistry and Molecular Biology Education 10.1002/bmb.20948


Whitworth, D. 2015. An off-the-shelf, authentic and versatile undergraduate molecular biology practical course. Biochemistry and Molecular Biology Education 43 (6) pp. 434-440. 10.1002/bmb.20893

Whitworth, D.E., Slade, S.E., Mironas, A. 2015. Composition of distinct sub-proteomes in Myxococcus xanthus: metabolic cost and amino acid availability. Amino Acids 47 (12) pp. 2521-2531. 10.1007/s00726-015-2042-x

Whitworth, D.E. 2015. Genome-wide analysis of myxobacterial two-component systems: genome relatedness and evolutionary changes. BMC Genomics 16 (1) 780 10.1186/s12864-015-2018-y

Kara, A., Vickers, M., Swain, M., Whitworth, D.E., Fernandez-Fuentes, N. 2015. Genome-wide prediction of prokaryotic two-component system networks using a sequence-based meta-predictor. BMC Bioinformatics 16 (1) 297 10.1186/s12859-015-0741-7

Ortet, P., Whitworth, D.E., Santaella, C., Achouak, W., Barakat, M. 2015. P2CS: updates of the prokaryotic two-component systems database. Nucleic Acids Research 43 ((D1)) pp. D536-D541. 10.1093/nar/gku968

Whitworth, D., Morgan, B. 2015. Synergism between bacterial GAPDH and OMVs: disparate mechanisms but co-operative action. Frontiers in Microbiology 6 1231 10.3389/fmicb.2015.01231


Whitworth, D.E., Wright, K. 2014. Online assessment of learning and engagement in university laboratory practicals. British Journal of Educational Technology 10.1111/bjet.12193

Mela, S., Whitworth, D.E. 2014. The fist bump: a more hygienic alternative to the handshake. American Journal of Infection Control 42 (8) pp. 916-917. 10.1016/j.ajic.2014.04.011


Barakat, M., Ortet, P., Whitworth, D.E. 2013. P2RP: a Web-based framework for the identification and analysis of regulatory proteins in prokaryotic genomes. BMC Genomics 14 (1) pp. 269. 10.1186/1471-2164-14-269


Ortet, P., De Luca, G., Whitworth, D.E., Barakat, M. 2012. P2TF: a comprehensive resource for analysis of prokaryotic transcription factors. BMC Genomics 13 628 10.1186/1471-2164-13-628

Evans, A.G.L., Davey, H.M., Cookson, A.R., Currinn, H., Cooke-Fox, G., Stanczyk, P.J., Whitworth, D.E. 2012. Predatory activity of Myxococcus xanthus outer-membrane vesicles and properties of their hydrolase cargo. Microbiology 158 (Pt 11) pp. 2742-2752. 10.1099/mic.0.060343-0


Whitworth, D.E. 2011. Myxobacterial Vesicles: Death at a Distance? Advances in Applied Microbiology 75 pp. 1-31. 10.1016/B978-0-12-387046-9.00001-3


Evans, A.R., Whitworth, D.E. 2010. Correlations between the role, sequence conservation, genomic location and severity of phenotype in myxobacterial developmental genes. FEMS Microbiology Letters 312 (1) pp. 40-45. 10.1111/j.1574-6968.2010.02092.x Cadair

Cock, P.J.A., Whitworth, D.E. 2010. Evolution of relative reading frame bias in unidirectional prokaryotic gene overlaps. Molecular Biology and Evolution 27 (4) pp. 753-756. 10.1093/molbev/msp302 Cadair

Barakat, M., Ortet, P., Whitworth, D.E. 2010. P2CS: a database of prokaryotic two-component systems. Nucleic Acids Research 39 (1) pp. D771-D776. 10.1093/nar/gkq1023

Holmes, A.B., Kalvala, S., Whitworth, D.E. 2010. Spatial simulations of myxobacterial development. PLoS Computational Biology 6 (2) e1000686 10.1371/journal.pcbi.1000686 Other

Williams, R.H.N., Whitworth, D.E. 2010. The genetic organisation of prokaryotic two-component system signalling pathways. BMC Genomics 11 (720) 720 pp. 1-12. 10.1186/1471-2164-11-720 Cadair Other


Whitworth, D.E., Cock, P.J.A. 2009. Evolution of prokaryotic two-component systems: insights from comparative genomics. Amino Acids 37 (3) pp. 459-466. 10.1007/s00726-009-0259-2 Cadair

Whitworth, D.E., Young, M., McEwan, N.R., Newbold, C.J., 2009. Life with little oxygen: lessons from microbes. IBERS Knowledge-based Innovations pp. 19-22. Other

Holmes, A.B., Kalvala, S., Whitworth, D.E. 2009. Myxobacteria Motility: a novel 3D model of rippling behaviour in Myxococcus xanthus. Systemics and Informatics World Network 6 pp. 65-70. Cadair Other

Barakat, M., Ortet, P., Jourlin-Castelli, C., Ansaldi, M., Méjean, V., Whitworth, D.E. 2009. P2CS: a two-component system resource for prokaryotic signal transduction research. BMC Genomics 10 315 10.1186/1471-2164-10-315 Cadair Other


Whitworth, D.E. 2008. Genomes and knowledge - a questionable relationship? Trends in Microbiology 16 (11) pp. 512-519. 10.1016/j.tim.2008.08.001 Cadair

Whitworth, D.E., Holmes, A.B., Irvine, A.G., Hodgson, D.A., Scanlan, D.J. 2008. Phosphate acquisition components of the Myxococcus xanthus Pho regulon are regulated by both phosphate availability and development. Journal of Bacteriology 190 (6) pp. 1997-2003. 10.1128/JB.01781-07 Cadair

Whitworth, D.E., Millard, A., Hodgson, D.A., Hawkins, P.F. 2008. Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus. Proteomics 8 (9) pp. 1839-1842. 10.1002/pmic.200700544 Cadair

Whitworth, D.E., Cock, P.J.A. 2008. Two-component systems of the myxobacteria: structure, diversity and evolutionary relationships. Microbiology 154 (2) pp. 360-372. 10.1099/mic.0.2007/013672-0 Cadair


Schneiker, S., Perlova, O., Kaiser, O., Gerth, K., Alici, A., Altmeyer, M.O., Bartels, D., Bekel, T., Beyer, S., Bode, E., Bode, H.B., Bolten, C.J., Choudhuri, J.V., Doss, S., Elnakady, Y.A., Frank, B., Gaigalat, L., Goesmann, A., Groeger, C., Gross, F., Jelsbak, L., Jelsbak, L., Kalinowski, J., Kegler, C., Knauber, T., Konietzny, S., Kopp, M., Krause, L., Krug, D., Linke, B., Mahmud, T., Martinez-Arias, R., McHardy, A.C., Merai, M., Meyer, F., Mormann, S., Muñoz-Dorado, J., Perez, J., Pradella, S., Rachid, S., Raddatz, G., Rosenau, F., Rückert, C., Sasse, F., Scharfe, M., Schuster, S.C., Suen, G., Treuner-Lange, A., Velicer, G.J., Vorhölter, F., Weissman, K.J., Welch, R.D., Wenzel, S.C., Whitworth, D.E., Wilhelm, S., Wittmann, C., Blöcker, H., Pühler, A., Müller, R. 2007. Complete genome sequence of the myxobacterium Sorangium cellulosum. Nature Biotechnology 25 (11) pp. 1281-1289. 10.1038/nbt1354

Cock, P.J.A., Whitworth, D.E. 2007. Evolution of gene overlaps: relative reading frame bias in prokaryotic two-component system genes. Journal of Molecular Evolution 64 (4) pp. 457-462. 10.1007/s00239-006-0180-1

Cock, P.J.A., Whitworth, D.E. 2007. Evolution of prokaryotic two-component system signaling pathways: Gene fusions and fissions. Molecular Biology and Evolution 24 (11) pp. 2355-2357. 10.1093/molbev/msm170 Other Cadair

Whitworth, D.E. 2007. Myxobacteria: Multicellularity and Differentiation. 1stth edn, ASM Press

Whitworth, D.E., Cock, P.J.A. 2007. Two-Component Signal Transduction System of the Myxobacteria. In D. Whitworth (ed), Myxobacteria: Multicellularity and Differentiation. ASM Press pp. 169-189.


Pham, V.D., Shebelut, C.W., Jose, I.R., Hodgson, D.A., Whitworth, D.E., Singer, M. 2006. The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus. Microbiology 152 (6) pp. 1609-1620. 10.1099/mic.0.28820-0


Higgs, P.I., Cho, K., Whitworth, D.E., Evans, L.S., Zusman, D.R. 2005. Four Unusual Two-Component Signal Transduction Homologs, RedC to RedF, Are Necessary for Timely Development in Myxococcus xanthus. Journal of Bacteriology 187 (23) pp. 8191-8195. 10.1128/JB.187.23.8191-8195.2005 Other Cadair


Whitworth, D.E., Bryan, S.J., Berry, A.E., McGowan, S.J., Hodgson, D.A. 2004. Genetic dissection of the light-inducible carQRS promoter region of Myxococcus xanthus. Journal of Bacteriology 186 (23) pp. 7836-7846. 10.1128/JB.186.23.7836-7846.2004 Other Cadair


Browning, D.F., Whitworth, D.E., Hodgson, D.A. 2003. Light-induced carotenogenesis in Myxococcus xanthus functional characterization of the ECF sigma factor CarQ and antisigma factor CarR. Molecular Microbiology 48 (1) pp. 237-251. 10.1046/j.1365-2958.2003.03431.x


Whitworth, D.E., Hodgson, D.A. 2001. Light-induced carotenogenesis in Myxococcus xanthus: evidence that CarS acts as an anti-repressor of CarA. Molecular Microbiology 42 (3) pp. 809-819. 10.1046/j.1365-2958.2001.02679.x Other Cadair