Photograph of Dr Alison Kingston-Smith.Dr Alison Kingston-Smith

Principal Investigator
PhD

Contact

Email: ahk@aber.ac.uk
Office: Penglais
Phone: 01970 823062

Research

Broad background in plant biochemistry and enzymology plus more limited experience of molecular biology. Particular areas of interest include photosynthesis (carbon metabolism/carbohydrates), antioxidant systems and plant stress responses culminating in cell death. Current research is concerned with the impact of post-ingestion plant metabolism on rumen function.

Staff Links

Publications

2016

Huws, S., Edwards, J.E., Creevey, C., Rees Stevens, P., Lin, W., Girdwood, S., Pachebat, J., Kingston-Smith, A. 2016. Temporal dynamics of the metabolically active rumen bacteria colonising fresh perennial ryegrass. FEMS Microbiology Ecology 92 (1) 10.1093/femsec/fiv137

Hart, E., Onime, L.A., Davies, T., Morphew, R., Kingston-Smith, A. 2016. The effects of PPO activity on the proteome of ingested red clover and implications for improving the nutrition of grazing cattle. Journal of Proteomics 141 pp. 67-76. 10.1016/j.jprot.2016.04.023

2015

Boeckx, T., Winters, A., Webb, J., Kingston-Smith, A. 2015. Polyphenol oxidase in leaves; is there any significance to the chloroplastic localization? Journal of Experimental Botany 66 (12) pp. 3571-3579. 10.1093/jxb/erv141

Boeckx, T., Webster, R., Winters, A., Webb, J., Gay, A., Kingston-Smith, A. 2015. Polyphenol oxidase-mediated protection against oxidative stress is not associated with enhanced photosynthetic efficiency. Annals of Botany 116 (4) pp. 529-540. 10.1093/aob/mcv081

Zampini, M., Rees Stevens, P., Pachebat, J., Kingston-Smith, A., Mur, L., Hayes, F. 2015. RapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli. Scientific Reports 5 11302 10.1038/srep11302

2014

Sanchez-Martin, J., Heald, J., Kingston-Smith, A., Winters, A., Rubiales, D., Sanz, M.., Mur, L., Prats, E. 2014. A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based on salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism. Plant, Cell and Environment 10.1111/pce.12501

A Huws, S., Mayorga, O.L., Theodorou, M.K., Kim, E.J., Cookson, A., Newbold, J., Kingston-Smith, A.H. 2014. Differential Colonization of Plant Parts by the Rumen Microbiota is likely to be due to Different Forage Chemistries. Journal of Microbial and Biochemical Technology 6 (2) pp. 80-86. 10.4172/1948-5948.1000126 Other

Claes, J., Davies, T., Rees Stevens, P., Wilkinson, T., Mur, L., Kingston-Smith, A. 2014. The effect of resistance to mildew infection on ruminal fermentation of Lolium perenne. In A. Hopkins (ed), EGF at 50: The future of European grasslands. Proceedings of the 25th General Meeting of the European Grassland Federation, Aberystwyth, Wales, 7-11 September 2014. : Grassland Science in Europe. Proceedings of the 25th General Meeting of the European Grassland Federation, Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 07/09/2014 - 11/09/2014. Prifysgol Aberystwyth | Aberystwyth University pp. 849.

Humphreys, M.W., O'Donovan, S.A., Farrell, M.S., Gay, A.P., Kingston-Smith, A.H. 2014. The potential of novel Festulolium (2n=4x=28) hybrids as productive, nutrient-use-efficient fodder for ruminants. Food and Energy Security 3 (2) pp. 98-110. 10.1002/fes3.50 Other

2013

Kingston-Smith, A.H., Marshall, A.H., Moorby, J.M. 2013. Breeding for genetic improvement of forage plants in relation to increasing animal production with reduced environmental footprint. animal 7 (Supplement s1) International Symposium on the Nutrition of Herbivores (ISNH8), Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 06/09/2011 - 09/09/2011. pp. 79-88. 10.1017/S1751731112000961

Kingston-Smith, A.H., Davies, T., Rees Stevens, P., Mur, L.A.J. 2013. Comparative Metabolite Fingerprinting of the Rumen System during Colonisation of Three Forage Grass (Lolium perenne L.) Varieties. PLoS One 8 (11) e82801 10.1371/journal.pone.0082801

Humphreys, M., Farrell, M., Detheridge, A., Scullion, J., Kingston-Smith, A., O'Donovan, S.A. 2013. Resilient and multifunctional grasslands for agriculture and environmental service during a time of climate change. In Á. Helgadóttir, A. Hopkins (eds), The Role of Grasslands in a Green Future: Threats and Perspectives in Less Favoured Areas. European Grassland Federation pp. 335-337.

Huws, S.A., Griffith, G.W., Edwards, J.E., Williams, H.W., James, W.P., Owen, I.G., Kingston-Smith, A.H. 2013. Sicrhau argaeledd cynnyrch cil gnowyr o'r ansawdd orau mewn modd effethlon. Gwerddon 13 pp. 10-28.

Huws, S.A., Mayorga, O.L., Theodorou, M.K., Onime, L.A., Kim, E.J., Cookson, A.H., Newbold, C.J., Kingston-Smith, A.H. 2013. Successional colonization of perennial ryegrass by rumen bacteria. Letters in Applied Microbiology 56 (3) pp. 186-196. 10.1111/lam.12033

2012

Kingston-Smith, A.H., Davies, T.E., Edwards, J.E., Gay, A., Mur, L.A.J. 2012. Evidence of a role for foliar salicylic acid in regulating the rate of post-ingestive protein breakdown in ruminants and contributing to landscape pollution. Journal of Experimental Botany 63 (8) pp. 3243-3255. 10.1093/jxb/ers048

Huws, S.A., Lee, M.R.F., Kingston-Smith, A.H., Kim, E.J., Scott, M.B., Tweed, J., Scollan, N.D. 2012. Ruminal protozoal contribution to the duodenal flow of fatty acids following feeding of steers on forages differeing in chloroplast content. British Journal of Nutrition 108 (12) pp. 2207-2214. 10.1017/S0007114512000335

O'Donovan, S.A., Kingston-Smith, A., Humphreys, M. 2012. Understanding the Genetic Basis for Slow Plant-Mediated Proteolysis in Festulolium Hybrids. In S. Barth, D. Milbourne (eds), Breeding strategies for sustainable forage and turf grass improvement. Springer pp. 89-95. 10.1007/978-94-007-4555-1_11

2011

Boeckx, T., Webster, R., Winters, A.L., Webb, K.J., Kingston-Smith, A.H. 2011. Can PPO mitigate photooxidative damage under abiotic stress conditions? 2011 Society for Experimental Biology Annual Main Meeting, Glasgow, , 01/07/2011 - 04/07/2011. pp. 34.

Huws, S.A., Kingston-Smith, A.H., Kim, E.J., Lee, M.R.F., Scott, M.B.S., Scollan, N.D. 2011. Contribution of ruminal protozoa to the duodenal flow of polyunsaturated fatty acids following feeding on low and high chloroplast-containing diets. ASF/AVTR/BSAS/WPSA Annual Conference, 'Food Security - Challenges and Opportunities for Animal Science, Nottingham University, , 04/04/2011 - 05/04/2011.

Simova-Stoilova, L.P., Demirevska, K.N., Kingston-Smith, A.H., Feller, U. 2011. Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Science 183 pp. 43-49. 10.1016/j.plantsci.2011.11.006

2010

Mur, L.A.J., Aubry, S., Mondhe, M., Kingston-smith, A., Gallagher, J., Timms-taravella, E., James, C., Papp, I., H??rtensteiner, S., Thomas, H., Ougham, H. 2010. Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis. New Phytologist 188 (1) pp. 161-174. 10.1111/j.1469-8137.2010.03377.x Cadair Other

Edwards, J.E., Yeoman, C.J., Huws, S.A., Kingston-Smith, A.H., Mackie, R.I. 2010. Biofilm formation and growth dynamics of Prevotella ruminicola during the colonisation of fresh perennial ryegrass. Gut Microbiology: New insights into gut microbial ecosystems, 7th Joint Symposium Rowett-INRA Meeting, Aberdeen Exhibition and Conference Centre, , 23/06/2010 - 25/06/2010.

Huws, S.A., Kingston-Smith, A.H., Kim, E.J., Lee, M.R.F., Scott, M.B., Tweed, J.K.S., Scollan, N.D. 2010. Contribution of ruminal protozoa to the duodenal flow of polyunsaturated fatty acids as a consequence of intracellular chloroplasts.

Huws, S.A., Kingston-Smith, A.H., Kim, E.J., Lee, M.R.F., Scott, M.B., Tweed, J.K.S., Scollan, N.D. 2010. Contribution of ruminal protozoa to the duodenal flow of polyunsaturated fatty acids following feeding on low and high chloroplasts. ProSafeBeef Annual General Meeting - EU Framework 6, Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 05/10/2010 - 07/10/2010. pp. p 53.

Kingston-Smith, A.H., Edwards, J.E., Huws, S.A., Kim, E.J., Abberton, M.T. 2010. Plant-based strategies towards minimising livestock's long shadow. Proceedings of the Nutrition Society 69 (4) The Winter Meeting of the Nutrition Society, Reading, United Kingdom of Great Britain and Northern Ireland, 15/12/2009 - 15/12/2009. pp. 613-620. 10.1017/S0029665110001953 Cadair

Yeoman, C.J., Edwards, J.E., Kingston-Smith, A.H., Mackie, R.I. 2010. Transcriptional responses of Prevotella ruminicola reflect spatial and temporal differences in available nutrients from fresh perennial ryegrass. 7th Joint Symposium INRA-AU Meeting 2010, Aberdeen, , 23/06/2010 - 25/06/2010.

Kingston-Smith, A.H. 2010. When being nice isn't enough - gender responsibility and equitable career progression. SEBulletin pp. 10-11. Other Other

2009

Edwards, J.E., Morris, P., Robbins, M.P., Kim, E.J., Kingston-Smith, A.H. 2009. Bacterial colonisation of the tanniferous forage lotus corniculatus in the bovine rumen. pp. 569-570. 10.1007/s00248-009-9496-x Cadair

Jimenez, H.R., Kingston-Smith, A.H., McEwan, N.R., Sanderson, R., Theodorou, M.K., Van Houten, J.E., McEwan, N.R., Edwards, J.E. 2009. Effect of fresh forage particle length on anaerobic fungal metabolism in vitro. Functionality, interaction with the host and impact on the environment. GUT MICROBIOME: Functionality, interaction with the host and impact on the environment, Clermont-Ferrand, France, 18/06/2008 - 20/06/2008. pp. 18-20. Cadair Other

Moorby, J.M., Kingston-Smith, A.H., Abberton, M.T., Humphreys, M.O., Theodorou, M.K. 2009. Improvement of forages to increase the efficiency of nitrogen and energy use by ruminants. In P. Garnsworthy, J. Wiseman (eds), Recent Advances in Animal Nutrition 2008. 42nd Feed Conference, Nottingham, United Kingdom of Great Britain and Northern Ireland, 02/09/2008 - 04/09/2008. Nottingham University Press pp. 39-65. Cadair Other

Huws, S.A., Kim, E.J., Kingston-Smith, A.H., Lee, M.R., Muetzel, S.M., Cookson, A.R., Newbold, J., Wallace, R.J., Scollan, N.D. 2009. Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts. FEMS Microbiology Ecology 69 (3) pp. 461-471. 10.1111/j.1574-6941.2009.00717.x Cadair

Kingston-Smith, A., Davies, T., Kyme, E., Mur, L. 2009. The phytohormone salicylic acid influences autolysis in ruminantingested plant cells. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology 153 (2 (Supplement)) S160 10.1016/j.cbpa.2009.04.323 Cadair

Newbold, C.J., Scollan, N.D., Edwards, J.E., Kingston-Smith, A.H., Kim, E.J. 2009. The plant-microbe interactome in ruminants: identification of control for mitigation of negative ecosystem outputs. Aspects of Applied Biology 98 Proceedings of International Conference on Positive Plant Microbial Interactions in Relation to Plant Performance and Ecosystem Function, Grantham, United Kingdom of Great Britain and Northern Ireland, 15/12/2009 - 16/12/2009. pp. 91-99. Cadair

Yeoman, C.J., Edwards, J.E., Cann, I.K., Morrison, M., Kingston-Smith, A.H., Mackie, R.I. 2009. Transcriptional analyses of carbohydrate metabolism in Prevotella ruminicola.

2008

Edwards, J.E., Huws, S.A., Kim, E.J., Lee, M.R.F., Kingston-Smith, A.H., Scollan, N.D. 2008. Advances in microbial ecosystem concepts and their consequences for ruminant agriculture. animal 2 (5) pp. 653-660. 10.1017/S1751731108002164 Cadair

Kingston-Smith, A.H., Sanderson, R., Edwards, J.E., Jones, S. 2008. Bacterial colonisation of fresh and dried perennial ryegrass in the rumen. GUT MICROBIOME: Functionality, interaction with the host and impact on the environment. GUT MICROBIOME: Functionality, interaction with the host and impact on the environment, Clermont-Ferrand, France, 18/06/2008 - 20/06/2008. pp. 18-20. Cadair Other

Edwards, J.E., Huws, S.A., Kim, E.J., Kingston-Smith, A.H. 2008. Characterization of the dynamics of initial bacterial colonization of nonconserved forage in the bovine rumen. FEMS Microbiology Ecology 63 (1) pp. 141-142. 10.1111/j.1574-6941.2007.00413.x

Mayorga, O.L., Huws, S.A., Kim, E.J., Kingston-Smith, A.H., Cookson, A.H., Newbold, C.J., Theodorou, M.K. 2008. Differential colonisation of abaxial and adaxial surfaces of fresh perennial ryegrass leaves by rumen bacteria. Abstract, INRA-RRI 2008, Clermont Ferrand, France, , 18/06/2008 - 20/06/2008.

Edwards, J.E., Kingston-Smith, A.H., Jimenez, H.R., Huws, S.A., Skot, K.P., Griffith, G.W., McEwan, N.R., Theodorou, M.K. 2008. Dynamics of initial colonisation of non-conserved perennial ryegrass by anaerobic fungi in the bovine rumen. FEMS Microbiology Ecology 66 (3) pp. 537-545. 10.1111/j.1574-6941.2008.00563.x Cadair Other

Kingston-Smith, A.H., Huws, S.A., Edwards, J.E., 2008. Exploiting the dynamic nature of the plant-microbe interactions in the rumen. IBERS Knowledge-based Innovations pp. 13-16. Other

Kingston-Smith, A.H., Davies, T.E., Edwards, J.E., Theodorou, M.K. 2008. From plants to animals: the role of plant cell death in ruminant herbivores. Journal of Experimental Botany 59 (3) pp. 521-532. 10.1093/jxb/erm326 Cadair

Theodorou, M.K., Kingston-Smith, A.H., Abberton, M.T. 2008. Improvement of forages to increase the efficiency of nitrogen and energy use in temperate pastoral livestock systems. Archiva Zootechnica 11 (4) pp. 5-20. Cadair

Abberton, M.T., Kingston-Smith, A.H., Theodorou, M.K. 2008. Improvement of forages to increase the efficiency of nutrient and energy use in temperate pastoral livestock systems. Archiva Zootechnica 11 (4) pp. 5-20.

Theodorou, M.K., Abberton, M.T., Kingston-Smith, A.H., Humphreys, M.O. 2008. Improving genotypes and understanding phenotypes: breeding forages for livestock and the environment. 7th International Symposium on the Nutrition of Herbivores (ISNH-7), Beijing, China, , 16/09/2007 - 22/09/2007. Cadair

Mayorga, O.L., Huws, S.A., Kim, E.J., Kingston-Smith, A.H., Newbold, C.J., Theodorou, M.K. 2008. In vitro production and composition of extracellular polymeric substances formed by ruminal bacteria on perennial ryegrass. Society for General Microbiology general meeting, Dublin, Ireland, 8-11 September, , 01/01/2008 - 01/01/2008. pp. p70. Other

Edwards, J.E., Jimenez, H.R., Jones, S., Sanderson, R., Kingston-Smith, A.H. 2008. Microbial colonisation and degradation of two different perennial ryegrass preparations in the rumen. UK Molecular Ecology Group, 14th Meeting, , 03/07/2008 - 04/07/2008.

Mayorga, O.L., Huws, S.A., Kim, E.J., Kingston-Smith, A.H., Cookson, A.H., Newbold, C.J., Theodorou, M.K. 2008. Temporal and spatial colonisation of rumen protozoa on fresh perennial ryegrass. Abstract, INRA-RRI 2008, Clermont Ferrand, France, , 18/06/2008 - 20/06/2008.

Kingston-Smith, A.H. 2008. Wisdom, justice and skill in science, engineering and technology: Are the objectives of the Athena Project mythology? Other

2007

Edwards, J.E., Huws, S.A., Kim, E.J., Kingston-Smith, A.H. 2007. Characterization of the dynamics of initial bacterial colonization of nonconserved forage in the bovine rumen. FEMS Microbiology Ecology 62 (3) pp. 323-335. 10.1111/j.1574-6941.2007.00392.x Cadair

Kingston-Smith, A.H., Davies, T., Mur, L.A.J. 2007. Post-ingestion induction of plant cell death: Regulation and implications. pp. 200-. 10.1016/j.cbpa.2007.01.443 Cadair

2006

Huws, S.A., Kim, E.J., Lee, M.R.F., Kingston-Smith, A.H., Scollan, N.D. 2006. Fatty acid composition of rumen protozoa: a link with chloroplast ingestion? Gut Microbiology: Research to Improve Health, Immune Response and Nutrition, United Kingdom of Great Britain and Northern Ireland, 21/06/2006 - 23/06/2006. pp. S98-S99. Cadair

Theodorou, M.K., Lee, M.R.F., Winters, A.L., Minchin, F.R., Morris, P., Kingston-Smith, A.H., MacRae, J.C. 2006. Polyphenols and their influence on gut function and health in ruminants: A review. Environmental Chemistry Letters 4 (3) pp. 121-126. 10.1007/s10311-006-0061-2 Cadair

Bollard, A.L., Kingston-Smith, A.H., Minchin, F.R. 2006. The effect of nitrogen status on the regulation of plant-mediated proteolysis in ingested forage: an assessment using non-nodulating white clover. Annals of Applied Biology pp. 35-42. 10.1111/j.1744-7348.2006.00069.x Cadair

2005

Merry, R.J., Leemans, D.K., Kingston-Smith, A.H., Theodorou, M.K., Thomas, H. 2005. Evidence in support of a role for plant-mediated proteolysis in the rumens of grazing animals. British Journal of Nutrition pp. 73-79. 10.1079/BJN20041303 Other Cadair

Kingston-Smith, A.H., Wilkins, P.W., 2005. Leaves of high yielding perennial ryegrass contain less aggregated Rubisco than S23. Cadair Other

Abberton, M.T., Kingston-Smith, A.H., Marshall, A.H., Sizer Coverdale, E., Williams, T.A. 2005. Quantifying the variation in protein content in white clover (Trifolium repens L.). In F. O'Mara, R. Wilkins, L. 't Mannetie, D. Lovett, P. Rogers, T. Boland (eds), XX International Grassland Conference: Offered Papers. XX International Grassland Congress, Dublin, Dublin, Ireland, 26/06/2005 - 01/07/2005. Wageningen Academic Publishers Cadair Other

Minchin, F.R., Bollard, A.L., Kingston-Smith, A.H. 2005. Stress-induced changes in protease composition are determined by nitrogen supply in non-nodulating white clover. Journal of Experimental Botany pp. 745-753. 10.1093/jxb/eri049 Cadair

Theodorou, M.K., Morris, P., Kingston-Smith, A.H., 2005. The death of plants in animals. Cadair Other

2004

Winters, A.L., Minchin, F.R., Davies, Z., Kingston-Smith, A.H., Theodorou, M.K., Griffith, G.W., Merry, R.J. 2004. Effects of manipulating the protein content of white clover on silage quality. Animal Feed Science and Technology 116 (3-4) pp. 3-4. 10.1016/j.anifeedsci.2004.07.002 Cadair

Shaw, R.K., Kingston-Smith, A.H., Jenkins, G.M., Humphreys, M.W. 2004. Imaging Festulolium chromosomal introgressions by genomic in situ hybridisation as an aid to rapid plant breeding. Society for Experimental Biology (SEB) Meeting, Edinburgh, United Kingdom of Great Britain and Northern Ireland, 29/03/2004 - 02/04/2004. Cadair

2003

Thomas, B.J., Husain, S.E., Nguyen-Quoc, B., Kingston-Smith, A.H., N'tchobo, H., Foyer, C.H. 2003. Changes in activities of acid invertase isoforms affect sugar accumulation and composition during ripening of tomato fruit. Journal of Horticultural Science and Biotechnology 78 (2) pp. 182-192. Cadair Other

Theodorou, M.K., Kingston-Smith, A.H., Davies, T., Bollard, A.L., Shaw, R.K. 2003. Correlations between protein crops and protease activity in forage crops. pp. 15-16. Cadair

Gardner, R.K., Kingston-Smith, A.H., Harper, J.A., Jenkins, G.M. 2003. Improving forage protein through the use of Festulolium introgressions. 7th British Grassland Society Research Conference, Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 01/09/2003 - 03/09/2003. pp. 1-3. Cadair

Brooks, A.E., Kingston-Smith, A.H., Thomas, B.J., Bollard, A.L., Theodorou, M.K. 2003. Nutrient availability during the early stages of colonization of fresh forage by rumen micro-organisms. New Phytologist pp. 119-130. Cadair Other

Kingston-Smith, A.H., Bollard, A.L., Armstead, I.P., Thomas, B.J., Theodorou, M.K. 2003. Proteolysis and cell death in clover leaves is induced by grazing. Protoplasma 220 (3-4) pp. 119-129. 10.1007/s00709-002-0044-5 Cadair

Kingston-Smith, A.H., Thomas, H.M. 2003. Strategies of plant breeding for improved rumen function. Annals of Applied Biology 142 (1) pp. 13-24. 10.1111/j.1744-7348.2003.tb00224.x Cadair

2002

Humphreys, M.O., Kingston-Smith, A.H., Theodorou, M.K., Bollard, A.L. 2002. An assessment of the ability of the stay-green phenotype in Lolium species to provide an improved protein supply for ruminants. Annals of Botany pp. 731-740. 10.1093/aob/mcf139 Other Cadair

Beha, E.M., Thomas, B.J., Theodorou, M.K., Kingston-Smith, A.H. 2002. Grass cells ingested by ruminants undergo autolysis which differs from senescence: implications for grass breeding targets and livestock production. Plant, Cell and Environment pp. 1299-1312. 10.1046/j.1365-3040.2002.00908.x Cadair

Thornton, B., Paterson, E., Kingston-Smith, A.H., Pratt, S.M., Sim, A. 2002. Reduced atmospheric CO2 affects nitrogen mobilisation in Festuca rubra. Physiologia Plantarum 116 (1) pp. 62-72. 10.1034/j.1399-3054.2002.1160108.x Cadair

Theodorou, M.K., Thomas, H.M., Bollard, A.L., Kingston-Smith, A.H., 2002. The potential to decrease nitrogen losses from pasture by manipulating plant metabolism. pp. 136-137. Cadair Other

2001

Theodorou, M.K., Kingston-Smith, A.H., Davies, D.R., Merry, R.J., 2001. Advances in silage quality in the 21st century. pp. 121-133. Cadair Other

Husain, S.E., Foyer, C.H., Kingston-Smith, A.H., Thomas, B.J. 2001. Invertase protein, but not activity, is present throughout development of Lycopersicon esculentum and L. pimpinellifolium fruit. New Phytologist 150 (1) pp. 73-81. 10.1046/j.1469-8137.2001.00064.x Cadair