Dr Alison Kingston-Smith
Phone: 01970 823062
Broad background in plant biochemistry and enzymology plus more limited experience of molecular biology. Particular areas of interest include photosynthesis (carbon metabolism/carbohydrates), antioxidant systems and plant stress responses culminating in cell death. Current research is concerned with the impact of post-ingestion plant metabolism on rumen function.
An Integrated Multi-Omics Approach Reveals the Effects of Supplementing Grass or Grass Hay with Vitamin E on the Rumen Microbiome and Its Function. Frontiers in Microbiology 7 905 10.3389/fmicb.2016.009052016.
Temporal dynamics of the metabolically active rumen bacteria colonising fresh perennial ryegrass. FEMS Microbiology Ecology 92 (1) fiv 137 10.1093/femsec/fiv1372016.
Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications. Scientific Reports 6 26572 10.1038/srep265722016.
The effects of PPO activity on the proteome of ingested red clover and implications for improving the nutrition of grazing cattle. Journal of Proteomics 141 pp. 67-76. 10.1016/j.jprot.2016.04.0232016.
Polyphenol oxidase in leaves; is there any significance to the chloroplastic localization? Journal of Experimental Botany 66 (12) pp. 3571-3579. 10.1093/jxb/erv1412015.
Polyphenol oxidase-mediated protection against oxidative stress is not associated with enhanced photosynthetic efficiency. Annals of Botany 116 (4) pp. 529-540. 10.1093/aob/mcv0812015.
RapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli. Scientific Reports 5 11302 10.1038/srep113022015.
A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based on salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism. Plant, Cell and Environment 10.1111/pce.125012014.
Differential Colonization of Plant Parts by the Rumen Microbiota is likely to be due to Different Forage Chemistries. Journal of Microbial and Biochemical Technology 6 (2) pp. 80-86. 10.4172/1948-5948.1000126 Other2014.
The effect of resistance to mildew infection on ruminal fermentation of Lolium perenne. In A. Hopkins (ed), EGF at 50: The future of European grasslands. Proceedings of the 25th General Meeting of the European Grassland Federation, Aberystwyth, Wales, 7-11 September 2014. : Grassland Science in Europe. Proceedings of the 25th General Meeting of the European Grassland Federation, Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 07/09/2014 - 11/09/2014. Prifysgol Aberystwyth | Aberystwyth University pp. 849.2014.
Breeding for genetic improvement of forage plants in relation to increasing animal production with reduced environmental footprint. animal 7 (Supplement s1) International Symposium on the Nutrition of Herbivores (ISNH8), Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 06/09/2011 - 09/09/2011. pp. 79-88. 10.1017/S17517311120009612013.
Comparative Metabolite Fingerprinting of the Rumen System during Colonisation of Three Forage Grass (Lolium perenne L.) Varieties. PLoS One 8 (11) e82801 10.1371/journal.pone.00828012013.
Resilient and multifunctional grasslands for agriculture and environmental service during a time of climate change. In Á. Helgadóttir, A. Hopkins (eds), The Role of Grasslands in a Green Future: Threats and Perspectives in Less Favoured Areas. European Grassland Federation pp. 335-337.2013.
Sicrhau argaeledd cynnyrch cil gnowyr o'r ansawdd orau mewn modd effethlon. Gwerddon 13 pp. 10-28.2013.
Successional colonization of perennial ryegrass by rumen bacteria. Letters in Applied Microbiology 56 (3) pp. 186-196. 10.1111/lam.120332013.
Applying next generation sequencing towards understanding microbial-plant interactions. Plant Molecular Biology, Gordon Conference, , 15/07/2012 - 20/07/2012.2012.
Bacterial cell-cell communication within the rumen. Gut Microbiota: Friend or Foe?, France, 01/06/2012 - 03/06/2012.2012.
Evidence of a role for foliar salicylic acid in regulating the rate of post-ingestive protein breakdown in ruminants and contributing to landscape pollution. Journal of Experimental Botany 63 (8) pp. 3243-3255. 10.1093/jxb/ers0482012.
Investigation of a rumen bacterial 'functional core' utilising a metatranscriptomics approach. Gut Microbiota: Friend or Foe?, France, 01/06/2012 - 03/06/2012.2012.
Ruminal protozoal contribution to the duodenal flow of fatty acids following feeding of steers on forages differing in chloroplast content. British Journal of Nutrition 108 (12) pp. 2207-2214. 10.1017/S00071145120003352012.
Understanding the Genetic Basis for Slow Plant-Mediated Proteolysis in Festulolium Hybrids. In S. Barth, D. Milbourne (eds), Breeding strategies for sustainable forage and turf grass improvement. Springer pp. 89-95. 10.1007/978-94-007-4555-1_112012.
Can PPO mitigate photooxidative damage under abiotic stress conditions? 2011 Society for Experimental Biology Annual Main Meeting, Glasgow, , 01/07/2011 - 04/07/2011. pp. 34.2011.
Contribution of ruminal protozoa to the duodenal flow of polyunsaturated fatty acids following feeding on low and high chloroplast-containing diets. ASF/AVTR/BSAS/WPSA Annual Conference, 'Food Security - Challenges and Opportunities for Animal Science, Nottingham University, , 04/04/2011 - 05/04/2011.2011.
Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Science 183 pp. 43-49. 10.1016/j.plantsci.2011.11.0062011.
Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis. New Phytologist 188 (1) pp. 161-174. 10.1111/j.1469-8137.2010.03377.x2010.
Biofilm formation and growth dynamics of Prevotella ruminicola during the colonisation of fresh perennial ryegrass. Gut Microbiology: New insights into gut microbial ecosystems, 7th Joint Symposium Rowett-INRA Meeting, Aberdeen Exhibition and Conference Centre, , 23/06/2010 - 25/06/2010.2010.
Contribution of ruminal protozoa to the duodenal flow of polyunsaturated fatty acids as a consequence of intracellular chloroplasts.2010.
Contribution of ruminal protozoa to the duodenal flow of polyunsaturated fatty acids following feeding on low and high chloroplasts. ProSafeBeef Annual General Meeting - EU Framework 6, Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 05/10/2010 - 07/10/2010. pp. p 53.2010.
Plant-based strategies towards minimising livestock's long shadow. Proceedings of the Nutrition Society 69 (4) The Winter Meeting of the Nutrition Society, Reading, United Kingdom of Great Britain and Northern Ireland, 15/12/2009 - 15/12/2009. pp. 613-620. 10.1017/S0029665110001953 Cadair2010.
Transcriptional responses of Prevotella ruminicola reflect spatial and temporal differences in available nutrients from fresh perennial ryegrass. 7th Joint Symposium INRA-AU Meeting 2010, Aberdeen, , 23/06/2010 - 25/06/2010.2010.
Effect of fresh forage particle length on anaerobic fungal metabolism in vitro. Functionality, interaction with the host and impact on the environment. GUT MICROBIOME: Functionality, interaction with the host and impact on the environment, Clermont-Ferrand, France, 18/06/2008 - 20/06/2008. pp. 18-20. Cadair Other2009.
Improvement of forages to increase the efficiency of nitrogen and energy use by ruminants. In P. Garnsworthy, J. Wiseman (eds), Recent Advances in Animal Nutrition 2008. 42nd Feed Conference, Nottingham, United Kingdom of Great Britain and Northern Ireland, 02/09/2008 - 04/09/2008. Nottingham University Press pp. 39-65. Cadair Other2009.
The phytohormone salicylic acid influences autolysis in ruminantingested plant cells. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology 153 (2 (Supplement)) S160 10.1016/j.cbpa.2009.04.323 Cadair2009.
The plant-microbe interactome in ruminants: identification of control for mitigation of negative ecosystem outputs. Aspects of Applied Biology 98 Proceedings of International Conference on Positive Plant Microbial Interactions in Relation to Plant Performance and Ecosystem Function, Grantham, United Kingdom of Great Britain and Northern Ireland, 15/12/2009 - 16/12/2009. pp. 91-99. Cadair2009.
Transcriptional analyses of carbohydrate metabolism in Prevotella ruminicola.2009.
Bacterial colonisation of fresh and dried perennial ryegrass in the rumen. GUT MICROBIOME: Functionality, interaction with the host and impact on the environment. GUT MICROBIOME: Functionality, interaction with the host and impact on the environment, Clermont-Ferrand, France, 18/06/2008 - 20/06/2008. pp. 18-20. Cadair Other2008.
Characterization of the dynamics of initial bacterial colonization of nonconserved forage in the bovine rumen. FEMS Microbiology Ecology 63 (1) pp. 141-142. 10.1111/j.1574-6941.2007.00413.x2008.
Differential colonisation of abaxial and adaxial surfaces of fresh perennial ryegrass leaves by rumen bacteria. Abstract, INRA-RRI 2008, Clermont Ferrand, France, , 18/06/2008 - 20/06/2008.2008.
Dynamics of initial colonisation of non-conserved perennial ryegrass by anaerobic fungi in the bovine rumen. FEMS Microbiology Ecology 66 (3) pp. 537-545. 10.1111/j.1574-6941.2008.00563.x Cadair Other2008.
Exploiting the dynamic nature of the plant-microbe interactions in the rumen. IBERS Knowledge-based Innovations pp. 13-16. Other2008.
Improvement of forages to increase the efficiency of nitrogen and energy use in temperate pastoral livestock systems. Archiva Zootechnica 11 (4) pp. 5-20. Cadair2008.
Improvement of forages to increase the efficiency of nutrient and energy use in temperate pastoral livestock systems. Archiva Zootechnica 11 (4) pp. 5-20.2008.
Improving genotypes and understanding phenotypes: breeding forages for livestock and the environment. 7th International Symposium on the Nutrition of Herbivores (ISNH-7), Beijing, China, , 16/09/2007 - 22/09/2007. Cadair2008.
In vitro production and composition of extracellular polymeric substances formed by ruminal bacteria on perennial ryegrass. Society for General Microbiology general meeting, Dublin, Ireland, 8-11 September, , 01/01/2008 - 01/01/2008. pp. p70. Other2008.
Microbial colonisation and degradation of two different perennial ryegrass preparations in the rumen. UK Molecular Ecology Group, 14th Meeting, , 03/07/2008 - 04/07/2008.2008.
Temporal and spatial colonisation of rumen protozoa on fresh perennial ryegrass. Abstract, INRA-RRI 2008, Clermont Ferrand, France, , 18/06/2008 - 20/06/2008.2008.
Wisdom, justice and skill in science, engineering and technology: Are the objectives of the Athena Project mythology? Other2008.
Biofilm formation by rumen microbes on fresh perennial ryegrass under in vitro anaerobic conditions. UK Molecular Microbial Ecology Group 13th Meeting, Liverpool, United Kingdom of Great Britain and Northern Ireland, 16/07/2007 - 17/07/2007.2007.
Microbial colonization and subsequent biofilm formation by ruminal microorganisms on fresh perennial ryegrass. Conference on Gastrointestinal Function (CGIF), Chicago, United States of America, 16/04/2007 - 18/04/2007.2007.
Temporal dynamics of microbial colonisation of forage in the rumen. 161st Meeting of the Society for General Microbiology meeting, United Kingdom of Great Britain and Northern Ireland, 03/09/2007 - 06/09/2007.2007.
The occurrence of biofilm communities in the ruminal ecosystem. The Biofilm Club, United Kingdom of Great Britain and Northern Ireland, 05/09/2007 - 07/09/2007. pp. 119-130.2007.
Throwing new light on the hypersensitive response. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology 146 (4) C2.9 Abstracts of the Annual Main Meeting of the Society for Experimental Biology, Glasgow, United Kingdom of Great Britain and Northern Ireland, 31/03/2007 - 04/04/2007. 10.1016/j.cbpa.2007.01.4452007.
Development and application of RNA and DNA based DGGE to study the bacterial colonisation of fresh perennial ryegrass in the rumen. RRI-INRA Gut Microbiology 2006, Aberdeen, United Kingdom of Great Britain and Northern Ireland, 21/06/2006 - 23/06/2006. pp. S14.2006.
Fatty acid composition of rumen protozoa: a link with chloroplast ingestion? Gut Microbiology: Research to Improve Health, Immune Response and Nutrition, United Kingdom of Great Britain and Northern Ireland, 21/06/2006 - 23/06/2006. pp. S98-S99. Cadair2006.
Rumen bacterial diversity and its consequences in livestock agriculture. Proceedings of the British Society of Animal Science Annual Conference, York, United Kingdom of Great Britain and Northern Ireland, 27/03/2006 - 29/03/2006. pp. 222.2006.
The effect of nitrogen status on the regulation of plant-mediated proteolysis in ingested forage: an assessment using non-nodulating white clover. Annals of Applied Biology pp. 35-42. 10.1111/j.1744-7348.2006.00069.x Cadair2006.
Quantifying the variation in protein content in white clover (Trifolium repens L.). In F. O'Mara, R. Wilkins, L. 't Mannetie, D. Lovett, P. Rogers, T. Boland (eds), XX International Grassland Conference: Offered Papers. XX International Grassland Congress, Dublin, Dublin, Ireland, 26/06/2005 - 01/07/2005. Wageningen Academic Publishers Cadair Other2005.
Imaging Festulolium chromosomal introgressions by genomic in situ hybridisation as an aid to rapid plant breeding. Society for Experimental Biology (SEB) Meeting, Edinburgh, United Kingdom of Great Britain and Northern Ireland, 29/03/2004 - 02/04/2004. Cadair2004.
Changes in activities of acid invertase isoforms affect sugar accumulation and composition during ripening of tomato fruit. Journal of Horticultural Science and Biotechnology 78 (2) pp. 182-192. Cadair Other2003.
Correlations between protein crops and protease activity in forage crops. pp. 15-16. Cadair2003.
Improving forage protein through the use of Festulolium introgressions. 7th British Grassland Society Research Conference, Aberystwyth, United Kingdom of Great Britain and Northern Ireland, 01/09/2003 - 03/09/2003. pp. 1-3. Cadair2003.
Grass cells ingested by ruminants undergo autolysis which differs from senescence: implications for grass breeding targets and livestock production. Plant, Cell and Environment pp. 1299-1312. 10.1046/j.1365-3040.2002.00908.x Cadair2002.
Invertase protein, but not activity, is present throughout development of Lycopersicon esculentum and L. pimpinellifolium fruit. New Phytologist 150 (1) pp. 73-81. 10.1046/j.1469-8137.2001.00064.x Cadair2001.
Overexpression of Mn-superoxide dismutase in maize leaves leads to increased monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase activities. Journal of Experimental Botany 51 (352) pp. 1867-1877. 10.1093/jexbot/51.352.18672000.
Tansley Review No. 118. Post-Ingestion Metabolism of Fresh Forage. New Phytologist 148 (1) pp. 37-55. Other2000.
Ascorbate Metabolism in Potato Leaves Supplied with Exogenous Ascorbate. Free Radical Research 31 (sup1) pp. 171-179. 10.1080/107157699003014711999.