Prof John Draper

Prof John Draper

Chair

IBERS

Contact Details

Profile

I currently manage the High Resolution Metabolomics Laboratory and am responsible for oversight of Enabling Technologies within IBERS including core facilities for Metabolomics, Next Generation Sequencing, Plant Phenomics and Bioinformatics.

Teaching

Research

From a technology perspective I am interested in the development of generic, high throughput phenotyping methodologies, based on global metabolite analysis (metabolomics) for use in a range of fields. My lab is concentrating on metabolite fingerprinting & profiling using high resolution mass spectrometry techniques.

I have established a range of collaborations with laboratories interested in the application of metabolomics technology in food & nutrition research & in plant pathology. We have developed high throughput methods (metabolite fingerprinting, data representation & data analysis) for compositional analysis & comparison of food raw materials. Alongside clinical/veterinary institutions & industry partners, we are currently researching methods for determining dietary exposure & individual responses to diet constituents in humans & domesticated animals from blood & urine analysis.

From a fundamental research perspective my team has pioneered the development of Brachypodium distachyon as a new model system for plant functional genomics. Together with local & international collaborators we played a key role in collecting & characterizing Brachypodium distachyon germplasm in terms of karyotype, comparative genomics, tissue culture behavior, transformation efficiency & pathogen interactions.

Currently I am developing a metabolomics platform for both metabolite identification & high throughput phenotyping in grasses & cereals based on the Brachypodium metabolome. A practical application of this research focuses on a study of metabolic reprogramming in B. distachyon, rice & barley during the biotrophic phases of interaction with rice blast. In collaboration with Nick Talbot (Univ. Exeter, UK) this project extends to an analysis of metabolome changes in Magnaporthae grisae during epidermal cell penetration & colonization.

My research is supported by grants from the BBSRC, The Foods Standards Agency & Industry.

Publications

Beckmann, M, Wilson, T, Zubair, H, Lloyd, AJ, Lyons, L, Phillips, H, Tailliart, K, Gregory, N, Thatcher, R, Garcia-Perez, I, Frost, G, Mathers, JM & Draper, J 2020, 'A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure', Molecular Nutrition and Food Research. https://doi.org/10.1002/mnfr.202000517
Lloyd, AJ, Willis, ND, Wilson, T, Zubair, H, Chambers, E, Garcia-Perez, I, Xie, L, Tailliart, K, Beckmann, M, Mathers, JC & Draper, J 2019, 'Addressing the pitfalls when designing intervention studies to discover and validate biomarkers or habitual dietary intake', Metabolomics, vol. 15, no. 5, 72. https://doi.org/10.1007/s11306-019-1532-3
Lloyd, A, Willis, ND, Wilson, T, Zubair, H, Xie, L, Chambers, ES, Garcia-Perez, I, Tailliart, K, Beckmann, M, Mathers, JC & Draper, J 2019, 'Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals', Molecular Nutrition and Food Research. https://doi.org/10.1002/mnfr.201900062
Maruvada, P, Lampe, JW, Wishart, DS, Barupal, D, Chester, DN, Dodd, D, Djoumbou-Feunang, Y, Dorrestein, PC, Dragsted, LO, Draper, J, Duffy, LC, Dwyer, JT, Emenaker, NJ, Fiehn, O, Gerszten, RE, Hu, FB, Karp, RW, Klurfeld, DM, Laughlin, MR, Little, AR, Lynch, CJ, Moore, SC, Nicastro, HL, O'Brien, DM, Ordovás, JM, Osganian, SK, Playdon, M, Prentice, R, Raftery, D, Reisdorph, N, Roche, HM, Ross, SA, Sang, S, Scalbert, A, Srinivas, PR & Zeisel, SH 2019, 'Perspective: Dietary Biomarkers of Intake and Exposure - Exploration with Omics Approaches', Advances in Nutrition. https://doi.org/10.1093/advances/nmz075
Wilson, T, Garcia-Perez, I, Posma, JM, Lloyd, A, Chambers, ES, Tailliart, K, Zubair, H, Beckmann, M, Mathers, JC, Holmes, E, Frost, G & Draper, J 2019, 'Spot and Cumulative Urine Samples Are Suitable Replacements for 24-Hour Urine Collections for Objective Measure of Dietary Exposure in Adults Using Metabolite Biomarkers', Journal of Nutrition. https://doi.org/10.1093/jn/nxz138
More publications on the Research Portal