Prof Karl Hoffmann

PhD

Additional Information

PROJECT WEBPAGES:

Flatworm Functional Genomics Initiative (FUGI): www.sanger.ac.uk/science/collaboration/flatworm-functional-genomics-initiative-fugi

Teaching

Research

My research revolves around trying to answer this question: How can fundamental studies of developmental biology & gene regulation guide our search for next generation anthelmintic solutions?

Parasitic worms cause some of the most disfiguring, debilitating & chronic infectious diseases of human & animal populations across the globe. Reliance on limited drug classes to treat affected individuals & the lack of available vaccines to induce protective immunity suggests that current experimental approaches in identifying urgently needed anthelmintics have yet to deliver sustainable solutions.

Utilising both hypothesis-led & discovery driven research approaches my laboratory is developing new strategies for controlling parasitic helminths of biomedical importance. Our area of expertise is Schistosoma mansoni, one of the three main trematode species responsible for the neglected tropical disease Schistosomiasis. More than 200 million people suffer from this chronic & debilitating disease, with 90% of all worldwide cases found in poverty-stricken areas of sub-Saharan Africa where up to 300,000 individuals die each year. Praziquantel is currently used to treat schistosomiasis, but the need for repetitive treatment in endemic communities, concerns over the development of resistance & an unknown mechanism of action all have contributed to the active search for new chemotherapeutic agents or a prophylactic vaccine.

To identify next generation anthelmintics we look for solutions in the interrelated disciplines of helminth developmental biology, epigenetics & gene regulation. Our research includes individual-gene & (epi) genome-wide investigations, supported by experimental models of schistosome infection & objective analyses of helminth viability & phenotype. We envision that our integrated approach will

Publications

Egesa, M, Hoffmann, K, Hokke, CH, Yazdanbakhsh, M & Cose, S 2017, 'Rethinking Schistosomiasis Vaccine Development: Synthetic Vesicles' Trends in Parasitology, vol 1671. DOI: 10.1016/j.pt.2017.07.007
Nur-e-Alam, M, Yousaf, M, Ahmed, S, Al-Sheddi, ES, Shah, I, Fazakerley, D, Bari, A, Ghabbour, HA, Threadgill, MD, Whatley, K, Hoffmann, K & Al-Rehaily, AJ 2017, 'Neoclerodane Diterpenoids from Reehal Fatima, Teucrium yemense' Journal of Natural Products. DOI: 10.1021/acs.jnatprod.7b00188
Adema, CM, Hillier, LW, Jones, CS, Loker, ES, Knight, M, Minx, P, Oliveira, G, Raghavan, N, Shedlock, A, do Amaral, LR, Arican-Goktas, HD, Assis, JG, Baba, EH, Baron, OL, Bayne, CJ, Bickham-Wright, U, Biggar, KK, Blouin, M, Bonning, BC, Botka, C, Bridger, JM, Buckley, KM, Buddenborg, SK, Lima Caldeira, R, Carleton, J, Carvalho, OS, Castillo, MG, Chalmers, IW, Christensens, M, Clifton, S, Cosseau, C, Coustau, C, Cripps, RM, Cuesta-Astroz, Y, Cummins, SF, Di Stephano, L, Dinguirard, N, Duval, D, Emrich, S, Feschotte, C, Feyereisen, R, Fitzgerald, P, Fronick, C, Fulton, L, Galinier, R, Gava, SG, Geusz, M, Geyer, KK, Giraldo-Calderón, GI, de Souza Gomes, M, Gordy, MA, Gourbal, B, Grunau, C, Hanington, PC, Hoffmann, KF, Hughes, D, Humphries, J, Jackson, DJ, Jannotti-Passos, LK, De Jesus Jeremias, W, Jobling, S, Kamel, B, Kapusta, A, Kaur, S, Koene, JM, Kohn, AB, Lawson, D, Lawton, SP, Liang, D, Limpanont, Y, Liu, S, Lockyer, AE, Lovato, TL, Ludolf, F, Magrini, V, Mcmanus, DP, Medina, M, Misra, M, Mitta, G, Mkoji, GM, Montague, MJ, Montelongo, C, Moroz, LL, Munoz-Torres, MC, Niazi, U, Noble, LR, Oliveira, FS, Pais, FS, Papenfuss, AT, Peace, R, Pena, JJ, Pila, EA, Quelais, T, Raney, BJ, Rast, JP, Rollinson, D, Rosse, IC, Rotgans, B, Routledge, EJ, Ryan, KM, Scholte, LLS, Storey, KB, Swain, M, Tennessen, JA, Tomlinson, C, Trujillo, DL, Volpi, EV, Walker, AJ, Wang, T, Wannaporn, I, Warren, WC, Wu, X-J, Yoshino, TP, Yusuf, M, Zhang, S-M, Zhao, M & Wilson, RK 2017, 'Whole genome analysis of a schistosomiasis-transmitting freshwater snail' Nature Communications, vol 8, 15451 (2017), pp. 15451. DOI: 10.1038/ncomms15451
Geyer, K, Niazi, U, Duval, D, Cosseau, C, Tomlinson, C, Chalmers, I, Swain, M, Cutress, D, Bickham-Wright, U, Munshi, SE, Grunau, C, Yoshino, TP & Hoffmann, K 2017, 'The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni' PLoS Neglected Tropical Diseases, vol 11, no. 5, e0005246. DOI: 10.1371/journal.pntd.0005246
Cosseau, C, Wolkenhauer, O, Padalino, G, Geyer, K, Hoffmann, K & Grunau, C 2017, '(Epi)genetic Inheritance in Schistosoma mansoni: A Systems Approach' Trends in Parasitology, vol 33, no. 4, pp. 285-294. DOI: 10.1016/j.pt.2016.12.002
Niazi, U, Geyer, K, Vickers, M, Hoffmann, K & Swain, M, DISMISS: MeDIP-seq: High-throughput sequencing, 2016, Software, OMICtools.
Niazi, U, Geyer, KK, Vickers, MJ, Hoffmann, K & Swain, M 2016, 'DISMISS: detection of stranded methylation in MeDIP-Seq data' BMC Bioinformatics, vol 17, 295. DOI: 10.1186/s12859-016-1158-7
Hoffmann, K 2015, Sharing expertise/data/reagents. in Accelerating R&D for Neglected Diseases through Global Collaborations: WIPO Re:Search Partnership Stories 2013-2015. 2nd edn, BIO Ventures for Global Health, Seattle, USA, pp. 23.
Nowacki, FC, Swain, MT, Klychnikov, OI, Niazi, U, Ivens, A, Quintana, JF, Hensbergen, PJ, Hokke, CH, Buck, AH & Hoffmann, KF 2015, 'Protein and small non-coding RNA-enriched extracellular vesicles are released by the pathogenic blood fluke Schistosoma mansoni' Journal of Extracellular Vesicles, vol 4, 28665. DOI: 10.3402/jev.v4.28665
Chalmers, IW, Fitzsimmons, CM, Brown, M, Pierrot, C, Jones, FM, Wawrzyniak, JM, Fernandez-Fuentes, N, Tukahebwa, EM, Dunne, DW, Khalife, J & Hoffmann, KF 2015, 'Human IgG1 Responses to Surface Localised Schistosoma mansoni Ly6 Family Members Drop following Praziquantel Treatment' PLoS Neglected Tropical Diseases, vol 9, no. 7, pp. e0003920. DOI: 10.1371/journal.pntd.0003920
Smit, CH, van Diepen, A, Nguyen, DL, Wuhrer, M, Hoffmann, KF, Deelder, AM & Hokke, CH 2015, 'Glycomic analysis of life stages of the human parasite Schistosoma mansoni reveals developmental expression profiles of functional and antigenic glycan motifs' Molecular and Cellular Proteomics, vol 14, no. 7, pp. 1750-1769. DOI: 10.1074/mcp.M115.048280
Reimers, N, Homann, A, Höschler, B, Langhans, K, Wilson, RA, Pierrot, C, Khalife, J, Grevelding, CG, Chalmers, IW, Yazdanbakhsh, M, Hoffmann, KF, Hokke, CH, Haas, H & Schramm, G 2015, 'Drug-Induced Exposure of Schistosoma mansoni Antigens SmCD59a and SmKK7' PLoS Neglected Tropical Diseases, vol 9, no. 3, e0003593. DOI: 10.1371/journal.pntd.0003593
Edwards, J, Brown, M, Peak, E, Bartholomew, B, Nash, RJ & Hoffmann, KF 2015, 'The Diterpenoid 7-Keto-Sempervirol, Derived from Lycium chinense, Displays Anthelmintic Activity against both Schistosoma mansoni and Fasciola hepatica' PLoS Neglected Tropical Diseases, vol 9, no. 3, e0003604. DOI: 10.1371/journal.pntd.0003604
Hoffmann, KF, Brindley, PJ & Berriman, M 2014, 'Halting harmful helminths: Vaccines and new drugs are needed to combat parasitic worm infections' Science, vol 346, no. 168, pp. 168-169. DOI: 10.1126/science.1261139
Yoshino, TP, Brown, M, Wu, X-J, Jackson, CJ, Ocadiz-Ruiz, R, Chalmers, IW, Kolb, M, Hokke, CH & Hoffmann, KF 2014, 'Excreted/secreted Schistosoma mansoni venom allergen-like 9 (SmVAL9) modulates host extracellular matrix remodelling gene expression' International Journal for Parasitology, vol 44, no. 8, pp. 551-563. DOI: 10.1016/j.ijpara.2014.04.002
Hai, Y, Edwards, J, Van Zandt, MC, Hoffmann, KF & Christianson, DW 2014, 'Crystal Structure of Schistosoma mansoni Arginase, a Potential Drug Target for the Treatment of Schistosomiasis' Biochemistry, vol 53, no. 28, pp. 4671-4684. DOI: 10.1021/bi5004519
Geyer, KK, Chalmers, IW, MacKintosh, ND, Hirst, JE, Geoghegan, R, Badets, M, Brophy, PM, Brehm, K & Hoffmann, KF 2013, 'Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes' BMC Genomics, vol 14, 462. DOI: 10.1186/1471-2164-14-462
Truscott, M, Evans, DA, Gunn, M & Hoffmann, KF 2013, 'Schistosoma mansoni Hemozoin Modulates Alternative Activation of Macrophages via Specific Suppression of Retnla Expression and Secretion' Infection and Immunity, vol 81, no. 1, pp. 133-142. DOI: 10.1128/IAI.00701-12
Chalmers, IW & Hoffmann, KF 2012, 'Platyhelminth venom allergen-like (VAL) proteins: revealing structural diversity, class specific features and biological associations across the phylum' Parasitology, vol 139, no. 10, pp. 1231-1245. DOI: 10.1017/S0031182012000704
Fitzsimmons, CM, Jones, FM, Stearn, A, Chalmers, IW, Hoffmann, KF, Wawrzyniak, J, Wilson, S, Kabatereine, NB & Dunne, DW 2012, 'The Schistosoma mansoni tegumental-allergen-like (TAL) protein family: influence of developmental expression on human IgE responses' PLoS Neglected Tropical Diseases, vol 6, no. 4, e1593. DOI: 10.1371/journal.pntd.0001593
Geyer, KK & Hoffmann, KF 2012, 'Epigenetics: a key regulator of platyhelminth developmental biology?' International Journal for Parasitology, vol 42, no. 3, pp. 221-224. DOI: 10.1016/j.ijpara.2012.02.003
Brehm, K, Carlton, JM & Hoffmann, KF 2012, 'Editorial: Parasite genomics and post-genomic activities: 21st century resources for the parasite immunologist' Parasite Immunology, vol 34, no. 2-3, pp. 47-49. DOI: 10.1111/j.1365-3024.2011.01346.x
Swain, MT, Larkin, DM, Caffrey, CR, Davies, SJ, Loukas, A, Skelly, PJ & Hoffmann, KF 2011, 'Schistosoma comparative genomics: integrating genome structure, parasite biology and anthelmintic discovery' Trends in Parasitology, vol 27, no. 12, pp. 555-564. DOI: 10.1016/j.pt.2011.09.003
Geyer, KK, Rodríguez-López, CM, Hoffmann, KF, Chalmers, IW, Munshi, SE, Truscott, M, Heald, JK, Wilkinson, MJ & Hoffmann, KF 2011, 'Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni' Nature Communications, vol 2, 424. DOI: 10.1038/ncomms1433
Peak, E & Hoffmann, K 2011, 'Cross-disciplinary approaches for measuring parasitic helminth viability and phenotype' Anais da Academia Brasileira de Ciências, vol 83, no. 2, pp. 649-662. DOI: 10.1590/S0001-37652011000200024
Peak, E & Hoffmann, KF 2011, Dual fluorescence assay., Patent No. WO2011/033290 A2.
Peak, E, Chalmers, IW & Hoffmann, KF 2010, 'Development and Validation of a Quantitative, High-Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability' PLoS Neglected Tropical Diseases, vol 4, no. 7, e759. DOI: 10.1371/journal.pntd.0000759
Fitzpatrick, JM, Peak, E, Perally, S, Chalmers, IW, Barrett, J, Yoshino, TP, Ivens, AC & Hoffmann, KF 2009, 'Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses' PLoS Neglected Tropical Diseases, vol 3, no. 11, e543.. DOI: 10.1371/journal.pntd.0000543
Fitzpatrick, JM, Fuentes, JM, Chalmers, IW, Wynn, TA, Modolell, M, Hoffmann, KF & Hesse, M 2009, 'Schistosoma mansoni arginase shares functional similarities with human orthologs but depends upon disulphide bridges for enzymatic activity' International Journal for Parasitology, vol 39, no. 3, pp. 267-279. DOI: 10.1016/j.ijpara.2008.06.015
Geyer, K, Rodrigues Lopez, CM, Wilkinson, MJ & Hoffmann, KF 2009, 'The Schistosoma mansoni genome is methylated' The Schistosome Molecular Toolbox Workshop, University of California, 2009 - 2009, .
Peak, E, Chalmers, IW & Hoffmann, KF 2009, 'Development and validation of a quantitative, high-throughput, fluorescent-based bioassay to detect Schistosoma viability' The Schistosome Molecular Toolbox Workshop, University of California, 2009 - 2009, .
Truscott, M & Hoffmann, KF 2009, 'Schistosoma mansoni haemozoin affects macrophage phenotype in a changing cytokine milieu' British Society for Parasitology, Spring Meeting, United Kingdom of Great Britain and Northern Ireland, 2009 - 2009, .
Lockyer, AE, Spinks, J, Kane, RA, Hoffmann, KF, Fitzpatrick, JM, Rollinson, D, Noble, LR & Jones, CS 2008, 'Biomphalaria glabrata transcriptome: cDNA microarray profiling identifies resistant- and susceptible-specific gene expression in haemocytes from snail strains exposed to Schistosoma mansoni' BMC Genomics, vol 9, 634. DOI: 10.1186/1471-2164-9-634
Fitzpatrick, JM, Protasio, AV, McArdle, AJ, Williams, GA, Johnston, DA & Hoffmann, KF 2008, 'Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae' PLoS Neglected Tropical Diseases, vol 2, no. 10, e323. DOI: 10.1371/journal.pntd.0000323
Chalmers, IW, McArdle, AJ, Coulson, RMR, Wagner, MA, Schmid, R, Hirai, H & Hoffmann, KF 2008, 'Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family' BMC Genomics, vol 9, no. 89, 89. DOI: 10.1186/1471-2164-9-89
Grevelding, CG & Hoffmann, KF 2007, 'Schistosome genomics and beyond: News and views' Experimental Parasitology, vol 117, no. 3, pp. 223-224. DOI: 10.1016/j.exppara.2007.09.001
Hokke, CH, Deelder, AM, Hoffmann, KF & Wuhrer, M 2007, 'Glycomics-driven discoveries in schistosome research' Experimental Parasitology, vol 117, no. 3, pp. 275-283. DOI: 10.1016/j.exppara.2007.06.003
Hokke, CH, Fitzpatrick, JM & Hoffmann, KF 2007, 'Integrating transcriptome, proteome and glycome analyses of Schistosoma biology' Trends in Parasitology, vol 23, no. 4, pp. 165-174. DOI: 10.1016/j.pt.2007.02.007
Fitzpatrick, JM, Hirai, Y, Hirai, H & Hoffmann, KF 2006, 'Schistosome egg production is dependent upon the activities of two developmentally regulated tyrosinases' FASEB Journal, vol 21, no. 3, pp. 823-835. DOI: 10.1096/fj.06-7314com
Wuhrer, M, Koeleman, CAM, Fitzpatrick, JM, Hoffmann, KF, Deelder, AM & Hokke, CH 2006, 'Gender-specific expression of complex-type N-glycans in schistosomes' Glycobiology, vol 16, no. 10, pp. 991-1006. DOI: 10.1093/glycob/cwl020
Hoffmann, KF, Caspar, P, Cheever, AW & Wynn, TA 1998, 'IFN-gamma, IL-12, and TNF-alpha are required to maintain reduced liver pathology in mice vaccinated with Schistosoma mansoni eggs and IL-12' Journal of Immunology, vol 161, no. 8, pp. 4201-4210.
Hoffmann, KF & Strand, M 1997, 'Molecular characterization of a 20.8-kDa Schistosoma mansoni antigen. Sequence similarity to tegumental associated antigens and dynein light chains' Journal of Biological Chemistry, vol 272, no. 23, pp. 14509-14515.
Hoffmann, KF & Strand, M 1996, 'Molecular identification of a Schistosoma mansoni tegumental protein with similarity to cytoplasmic dynein light chains' Journal of Biological Chemistry, vol 271, no. 42, pp. 26117-26123. DOI: 10.1074/jbc.271.42.26117
Fitzpatrick, JM & Hoffmann, KF 2006, 'Dioecious Schistosoma mansoni express divergent gene repertoires regulated by pairing' International Journal for Parasitology, vol 36, no. 10-11, pp. 1081-1089. DOI: 10.1016/j.ijpara.2006.06.007
Reimert, CM, Fitzsimmons, CM, Joseph, S, Mwatha, JK, Jones, FM, Kimani, G, Hoffmann, KF, Booth, M, Kabatereine, NB, Dunne, DW & Vennervald, BJ 2006, 'Eosinophil activity in Schistosoma mansoni infections in vivo and in vitro in relation to plasma cytokine profile pre- and posttreatment with praziquantel' Clinical and Vaccine Immunology, vol 13, no. 5, pp. 584-593. DOI: 10.1128/CVI.13.5.584-593.2006
Vermeire, JJ, Taft, AS, Hoffmann, KF, Fitzpatrick, JM & Yoshino, TP 2006, 'Schistosoma mansoni: DNA microarray gene expression profiling during the miracidium-to-mother sporocyst transformation' Molecular and Biochemical Parasitology, vol 147, no. 1, pp. 39-47. DOI: 10.1016/j.molbiopara.2006.01.006
Wynn, TA, Aliberti, J, Hoffmann, KF, Jankovic, D, Feng, CG, Kullberg, MC & Sher, A 2006, Experimental Models for the Analysis of IL-10 Function. in FM Marincola (ed.), Interleukin-10. Taylor & Francis.
Fitzsimmons, CM, Schramm, G, Jones, FM, Chalmers, IW, Hoffmann, KF, Grevelding, CG, Wuhrer, M, Hokke, CH, Haas, H, Doenhoff, MJ & Dunne, DW 2005, 'Molecular characterization of omega-1: A hepatotoxic ribonuclease from Schistosoma mansoni eggs' Molecular and Biochemical Parasitology, vol 144, no. 1, pp. 123-127. DOI: 10.1016/j.molbiopara.2005.08.003
Fitzpatrick, JM, Johnston, DA, Williams, GW, Williams, DJ, Freeman, TC, Dunne, DW & Hoffmann, KF 2005, 'An oligonucleotide microarray for transcriptome analysis of Schistosoma mansoni and its application/use to investigate gender-associated gene expression' Molecular and Biochemical Parasitology, vol 141, no. 1, pp. 1-13. DOI: 10.1016/j.molbiopara.2005.01.007
Fitzpatrick, JM & Hoffmann, KF 2005, The Application of DNA Microarrays in the Functional Genomic Study of Schistosome/Host Biology. in Schistosomiasis: World Class Parasites Volume 10. Springer Nature, pp. 101-112.
Fitzsimmons, CM, Stewart, TJ, Hoffmann, KF, Grogan, JL, Yazdanbakhsh, M & Dunne, DW 2004, 'Human IgE response to the Schistosoma haematobium 22.6 kDa antigen' Parasite Immunology, vol 26, no. 8-9, pp. 371-376. DOI: 10.1111/j.0141-9838.2004.00721.x
Fitzpatrick, JM, Johansen, MV, Johnston, DA, Dunne, DW & Hoffmann, KF 2004, 'Gender-associated gene expression in two related strains of Schistosoma japonicum' Molecular and Biochemical Parasitology, vol 136, no. 2, pp. 191-209. DOI: 10.1016/j.molbiopara.2004.03.014
Fitzsimmons, CM, Joseph, S, Jones, FM, Reimert, CM, Hoffmann, KF, Kazibwe, F, Kimani, G, Mwatha, JK, Ouma, JH, Tukahebwa, EM, Kariuki, HC, Vennervald, BJ, Kabatereine, NB & Dunne, DW 2004, 'Chemotherapy for schistosomiasis in Ugandan fishermen: Treatment can cause a rapid increase in interleukin-5 levels in plasma but decreased levels of eosinophilia and worm-specific immunoglobulin E' Infection and Immunity, vol 72, no. 7, pp. 4023-4030. DOI: 10.1128/IAI.72.7.4023-4030.2004
Hoffmann, KF & Fitzpatrick, JM 2004, Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays. in SE Melville (ed.), Parasite Genomics Protocols. 270 edn, Springer Nature, pp. 219-236.
Wynn, TA, Hesse, M, Sandler, NG, Kaviratne, M, Hoffmann, KF, Chiaramonte, MG, Reiman, R, Cheever, AW, Sypek, JP & Mentink-Kane, MM 2004, 'P-selectin suppresses hepatic inflammation and fibrosis in mice by regulating interferon gamma and the IL-13 decoy receptor' Hepatology, vol 39, no. 3, pp. 676-687. DOI: 10.1002/hep.20102
Hoffmann, KF & Dunne, DW 2003, 'Characterization of the Schistosoma transcriptome opens up the world of helminth genomics' Genome Biology, vol 5, 203. DOI: 10.1186/gb-2003-5-1-203
Hoffmann, KF, Johnston, DA & Dunne, DW 2002, 'Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling' Genome Biology, vol 3, no. 8, pp. RESEARCH0041. DOI: 10.1186/gb-2002-3-8-research0041
Schopf, LR, Hoffmann, KF, Cheever, AW, Urban, JF & Wynn, TA 2002, 'IL-10 is critical for host resistance and survival during gastrointestinal helminth infection' Journal of Immunology, vol 168, no. 5, pp. 2383-2392.
Park, MK, Hoffmann, KF, Cheever, AW, Amichay, D, Wynn, TA & Farber, JM 2001, 'Patterns of chemokine expression in models of Schistosoma mansoni inflammation and infection reveal relationships between type 1 and type 2 responses and chemokines in vivo' Infection and Immunity, vol 69, no. 11, pp. 6755-6768. DOI: 10.1128/IAI.69.11.6755-6768.2001
Hoffmann, KF, McCarty, TC, Segal, DH, Chiaramonte, M, Hesse, M, Davis, EM, Cheever, AW, Meltzer, PS, Morse, HC & Wynn, TA 2001, 'Disease fingerprinting with cDNA microarrays reveals distinct gene expression profiles in lethal type-1 and type-2 cytokine-mediated inflammatory reactions' FASEB Journal, vol 15, no. 13, pp. 2545-25477. DOI: 10.1096/fj.01-0306fje
Cheever, AW, Hoffmann, KF & Wynn, TA 2000, 'Immunopathology of schistosomiasis mansoni in mice and men' Immunology Today, vol 21, no. 9, pp. 465-466. DOI: 10.1016/S0167-5699(00)01626-1
Hoffmann, KF, Cheever, AW & Wynn, TA 2000, 'IL-10 and the dangers of immune polarization: excessive type 1 and type 2 cytokine responses induce distinct forms of lethal immunopathology in murine schistosomiasis' Journal of Immunology, vol 164, no. 12, pp. 6406-6416.
Hoffmann, KF, James, SL, Cheever, AW & Wynn, TA 1999, 'Studies with double cytokine-deficient mice reveal that highly polarized Th1- and Th2-type cytokine and antibody responses contribute equally to vaccine-induced immunity to Schistosoma mansoni' Journal of Immunology, vol 163, no. 2, pp. 927-938.