Prof Peter Brophy PhD

Prof Peter Brophy

Professor

Contact Details

Profile

I studied biochemistry at Glasgow and Stirling Universities before obtaining a PhD from Aberystwyth University on drug metabolism in parasitic worms. After holding a Medical Research Council research post at Aberystwyth (1987-1990) I completed further research training in biotechnology at the BioCentre in Leicester. In 1991 I was awarded a Wellcome Trust Fellowship based at Nottingham University exploring parasite defences against immune assault. A Senior Research Fellowship in the Leicester Pharmacy School (1994-97) provided opportunities in rational drug design and lead compound synthesis. I established the first proteomics laboratory dedicated to parasitic worm investigations during Readerships at the Universities of Aberystwyth and Liverpool (1998-2006). In 2007 I returned to Aberystwyth as a Professor in Parasitology and Founder Director of Postgraduate Studies of IBERS (2008-2013). During my career I have been an advisor in parasite drug discovery to the WHO and completed a number of industrial consultancies. I have published almost a 100 research papers/books/chapters on parasite biochemistry including the first experimental proteomics study in a parasitic worm, and resolved one of the first structures of a worm drug target and I have won research contracts worth over ?3 million on using functional genomics to address problems in parasitology (anthelmintic resistance, vaccine and biomarker discovery).

Teaching

Research

My main research interest is to resolve using functional genomics how liver and rumen fluke parasites interact with their respective host environments. To date, new parasite proteins have been discovered to support drug and vaccine development and diagnostics . I am also interested in developing new molecular tools to measure anthelmintic resistance in liver fluke and a protein biomarker panel has been identified to measure Triclabendazole (TCBZ) failure and resistance for new laboratory and faecal based field tests.

Publications

2017

Jones, R., Williams, H., Dalesman, S., Sinmidele, A., Thomas, R.K., Brophy, P. 2017. The prevalence and development of digenean parasites within their intermediate snail host, Galba truncatula, in a geographic area where the presence of Calicophoron daubneyi has recently been confirmed. Veterinary Parasitology 240 pp. 68-74. Cadair

Jones, R., Brophy, P., Mitchell, E.S., Williams, H. 2017. Rumen fluke Calicophoron daubneyi on Welsh farms: Prevalence, risk factors, and observations on co-infection with Fasciola hepatica. Parasitology 144 (2) pp. 237-247. Cadair

2016

Morphew, R., Wilkinson, T., MacKintosh, N., Jahndel, V., Paterson, S., McVeigh, P., Abbas Abidi, S.M., Saifullah, K., Raman, M., Ravikumar, G., LaCourse, E.J., Maule, A.G., Brophy, P. 2016. Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species. Journal of Proteome Research 15 (9) pp. 3308-3321. Cadair

McCammick, E., McVeigh, P., McCusker, P., Timson, D., Morphew, R., Brophy, P., Marks, N., Mousley, A., Maule, A. 2016. Calmodulin disruption impacts growth and motility in juvenile liver fluke. Parasites & Vectors 9 46 Cadair

2015

Jones, R., Williams, H., Dalesman, S., Brophy, P. 2015. Confirmation of Galba truncatula as an intermediate host snail forCalicophoron daubneyi in Great Britain, with evidence of alternative snail species hosting Fasciola hepatica. Parasites & Vectors 8 (656) Cadair

Huson, K.M., Brophy, P., Morphew, R., MacKintosh, N. 2015. Identification of the rumen fluke Calicophoron daubneyi infecting cattle in Wales. Advances in Animal Biosciences 6 (Special Issue 02) Proceedings of the British Society of Animal Science. pp. 183. Cadair

Hart, E., Brophy, P., Prescott, M., Bartley, D.J., Wolf, B., Hamilton, J.V. 2015. A new enabling proteomics methodology to investigate membrane associated proteins from parasitic nematodes: Case study using ivermectin resistant and ivermectin susceptible isolates of Caenorhabditis elegans and Haemonchus contortus. Veterinary Parasitology 207 (3-4) pp. 266-275. Cadair

2014

Ahammed Shareef, P.A., Brennan, G.P., McVeigh, P., Hannan Khan, M.A., Morphew, R.M., Mousley, A., Marks, N.J., Khalid Saifullah, M.K., Brophy, P.M., Maule, A.G., Abidi, S.M.A. 2014. Time-dependent tegumental surface changes in juvenile Fasciola gigantica in response to triclabendazole treatment in goat. Acta Tropica 136 pp. 108-117. Cadair

Morphew, R.M., MacKintosh, N.D., Hart, E.H., Prescott, M., LaCourse, E.J., Brophy, P.M. 2014. In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment. EuPA Open Proteomics 3 pp. 85-99. Cadair

McVeigh, P., McCammick, E., McCusker, P., Morphew, R.M., Mousley, A., Abidi, A., Saifullah, K.M., Muthusamy, R., Gopalakrishnan, R., Spithill, T.W., Dalton, J.P., Brophy, P.M., Marks, N.J., Maule, A.G. 2014. RNAi dynamics in juvenile Fasciola spp. liver flukes reveals the persistence of gene silencing in vitro. PLoS Neglected Tropical Diseases 8 (9) 0003185 Cadair

2013

Geyer, K.K., Chalmers, I.W., MacKintosh, N.D., Hirst, J.E., Geoghegan, R., Badets, M., Brophy, P.M., Brehm, K., Hoffmann, K.F. 2013. Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes. BMC Genomics 14 462 Cadair

Zafra, R., Pérez-Écija, R.A., Buffoni, L., Pacheco, I.L., Martínez-Moreno, A., Lacourse, E.J., Perally, S., Brophy, P.M., Pérez, J. 2013. Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica. Research in Veterinary Science 94 (3) pp. 602-609. Cadair

Morphew, R.M., Hamilton, C.M., Wright, H.A., Dowling, D.J., O'Neill, S.M., Brophy, P.M. 2013. Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40. Veterinary Parasitology 191 (3-4) pp. 379–385. Cadair

2012

Hart, E.H., Morphew, R.M., Bartley, D.J., Millares, P., Wolf, B.T., Brophy, P.M., Hamilton, J.V. 2012. The soluble proteome phenotypes of ivermectin resistant and ivermectin susceptible Haemonchus contortus females compared. Veterinary Parasitology 190 (1-2) pp. 104-113. Cadair

Brophy, P.M., Mackintosh, N., Morphew, R.M. 2012. Anthelmintic metabolism in parasitic helminths: proteomic insights. Parasitology 139 (9) pp. 1205-1217. Cadair

LaCourse, E.J., Perally, S., Morphew, R.M., Moxon, J.V., Prescott, M.C., Dowling, D.J., O'Neill, S.M., Kipar, A., Hetzel, U., Hoey, E., Zafra, R., Buffoni, L., Arévalo, J.P., Brophy, P.M. 2012. The Sigma Class glutathione transferase from the liver fluke Fasciola hepatica. PLoS Neglected Tropical Diseases 6 (5) e1666 Cadair

Millares, P., LaCourse, J., Perally, S., Ward, D., Prescott, M., Hodgkinson, J., Brophy, P.M., Rees, H. 2012. Proteomic profiling and protein identification by MALDI-TOF mass spectrometry in unsequenced parasitic nematodes. PLoS One 7 (3) e33590 Cadair

Morphew, R.M., Eccleston, N., Wilkinson, T.J., McGarry, J., Perally, S., Prescott, M., Ward, D., Williams, D., Paterson, S., Raman, M., Ravikumar, G., Khalid Saifullah, M., Abbas Abidi, S.M., McVeigh, P., Maule, A.G., Brophy, P.M., LaCourse, E.J. 2012. Proteomics and in silico approaches to extend understanding of the glutathione transferase superfamily of the tropical liver fluke Fasciola gigantica. Journal of Proteome Research 11 (12) pp. 5876–5889. Cadair

2011

Morphew, R.M., Wright, H.A., LaCourse, E.J., Porter, J., Barrett, J., Woods, D.J., Brophy, P.M. 2011. Towards delineating functions within the fasciola secreted cathepsin L protease family by integrating in vivo based sub-proteomics and phylogenetics. PLoS Neglected Tropical Diseases 5 (1) e-937

2010

Mendes, R.E., Zafra, R., Martinez-Moreno, A., Brophy, P., Pérez, J. 2010. Early Hepatic Changes in Goats Immunized with Recombinant Glutathione S Transferase (Sigma Class) and Challenged with Fasciola hepatica. Journal of Comparative Pathology 143 (4) pp. 350. Cadair

Chemale, G., Perally, S., LaCourse, E.J., Prescott, M.C., Jones, L.M., Ward, D., Meany, M., Hoey, E., Brennan, G.P., Fairweather, I., Trudgett, A., Brophy, P.M. 2010. Comparative proteomic analysis of Triclabendazole response in the liver fluke Fasciola hepatica. Journal of Proteome Research 9 (10) pp. 4940-4951.

Moxon, J.V., LaCourse, E.J., Wright, H.A., Perally, S., Prescott, M.C., Gillard, J.L., Barrett, J., Hamilton, J.V., Brophy, P.M. 2010. Proteomic analysis of embryonic Fasciola hepatica: Characterization and antigenic potential of a developmentally regulated heat shock protein. Veterinary Parasitology 169 (1-2) pp. 62-75.

Jones, L.M., Staffa, K., Perally, S., LaCourse, E.J., Brophy, P.M., Hamilton, J.V. 2010. Proteomic analyses of Caenorhabditis elegans dauer larvae and long-lived daf-2 mutants implicates a shared detoxification system in longevity assurance. Journal of Proteome Research 9 (6) pp. 2871-2881.

Zhan, B., Perally, S., Brophy, P.M., Xue, J., Goud, G., Liu, S., Deumic, V., de Oliveira, L., Bethony, J., Bottazzi, M.E., Jiang, D., Gillespie, P., Xiao, S., Gupta, R., Loukas, A., Ranjit, N., Lustigman, S., Hotez, P., Oksov, Y. 2010. Molecular Cloning, Biochemical Characterization, and Partial Protective Immunity of the Heme-Binding Glutathione S-Transferases from the Human Hookworm Necator americanus. Infection and Immunity 78 (4) pp. 1552-1563.

Moxon, J.V., Flynn, R., Golden, O., Hamilton, J.V., Mulcahy, G., Brophy, P.M. 2010. Immune responses directed at egg proteins during experimental infection with the liver fluke Fasciola hepatica. Parasite Immunology 32 (2) pp. 111-124.

Dowling, D.J., Hamilton, C.M., Donnelly, S., La Course, J., Brophy, P.M., Dalton, J., O'Neill, S.M. 2010. Major secretory antigens of the helminth Fasciola hepatica activate a suppressive dendritic cell phenotype that attenuates Th17 cells but fails to activate Th2 immune responses. Infection and Immunity 78 (2) pp. 793-801.

2009

Hamilton, C.M., Dowling, D.J., Loscher, C.E., Morphew, R.M., Brophy, P.M., O'Neill, S.M. 2009. The Fasciola hepatica tegumental antigen suppresses dendritic cell maturation and function. Infection and Immunity 77 (6) pp. 2488-2498.

LaCourse, E.J., Hernandez-Viadel, M., Jefferies, J.R., Svendsen, C., Spurgeon, D.J., Barrett, J., Morgan, A.J., Kille, P., Brophy, P.M. 2009. Glutathione transferase (GST) as a candidate molecular based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus. Environmental Pollution 157 (8-9) pp. 2459-2469.

2008

Perally, S., LaCourse, E.J., Campbell, A.M., Brophy, P.M. 2008. Heme transport and detoxification in nematodes: subproteomics evidence of differential role of glutathione transferases. Journal of Proteome Research 7 (10) pp. 4557-4565.

LaCourse, E.J., Perally, S., Hernandez-Viadel, M., Wright, H.A., Brophy, P.M. 2008. A Proteomics Approach To Quantify Protein Levels Following RNA Interference: Case Study with Glutathione Transferase Superfamily from the Model Metazoan Caenorhabditis elegans. Journal of Proteome Research 7 (8) pp. 3314-3318. Cadair

2007

Somboon, P., Severson, D.W., Lumjuan, N., Stevenson, B.J., Prapanthadara, L., Ranson, H., Brophy, P.M., Loftus, B.J. 2007. The Aedes aegypti glutathione transferase family. Insect Biochemistry and Molecular Biology 37 (10) pp. 1026-1035.

Morphew, R.M., Wright, H.A., LaCourse, E.J., Woods, D.J., Brophy, P.M. 2007. Comparative proteomics of excretory-secretory proteins released by the liver fluke Fasciola hepatica in sheep host bile and during in vitro culture ex host. Molecular and Cellular Proteomics 6 pp. 963-972.

Brennan, G.P., Fairweather, I., Trudgett, A., Hoey, E., ., M., Mcconville, M., Meaney, M., Robinson, M., McFerran, N., Ryan, L., Lanusse, C., Mottier, L., Alvarez, L., Solana, H., Virkel, G., Brophy, P. 2007. Understanding triclabendazole resistance. Experimental and Molecular Pathology 82 (2) pp. 104-109. Cadair

2006

Chemale, G., Morphew, R.M., Moxon, J.V., Morassuti, A.L., LaCourse, E.J., Barrett, J., Johnston, D.A., Brophy, P.M. 2006. Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. Proteomics 6 (23) pp. 6263-6273.

Morgan, C.T., LaCourse, E.J., Rushbrook, B.J., Greetham, D., Hamilton, J.V., Barrett, J., Bailey, K., Brophy, P.M. 2006. Plasticity demonstrated in the proteome of a parasitic nematode within the intestine of different host strains. Proteomics 6 (16) pp. 4633-4645.

Morphew, R.M., Barrett, J., Brophy, P.M. 2006. Flatworm parasite proteomics. In J. N. (ed) in. CABI Publishing pp. 327-.

2005

Schuller, D.J., Liu, Q., Kriksunov, I.A., Campbell, A.M., Barrett, J., Brophy, P.M., Hao, Q. 2005. Crystal structure of a new class of glutathione transferase from the model human hookworm nematode Heligmosomoides polygyrus. Proteins: Structure, Function and Genetics 61 (4) pp. 1024-1031.

Zhan, B., Liu, S., Perally, S., Xue, J., Fujiwara, R., Brophy, P.M., Xiao, S., Liu, Y., Feng, J., Williamson, A., Wang, Y., Bueno, L.L., Mendez, S., Goud, G., Bethony, J.M., Hawdon, J.M., Loukas, A., Jones, K., Hotez, P.J. 2005. Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum. Infection and Immunity 73 (10) pp. 6903-6911.

Barrett, J., Brophy, P.M., Hamilton, J.V. 2005. Analysing proteomic data. International Journal for Parasitology 35 (5) pp. 543-553.

Chemale, G., Barrett, J., Zaha, A., Ferreira, H.B., Brophy, P.M. 2005. Echinococcus granulosus antigen B hydrophobic ligand binding properties. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics pp. 189-194.

2004

Greetham, D., Morgan, C.T., Campbell, A.M., van Rossum, A.J., Barrett, J., Brophy, P.M. 2004. Evidence of glutathione transferase complexing and signaling in the model nematode Caenorhabditis elegans using a pull-down proteomic assay. Proteomics 4 (7) pp. 1989-1995.

Brophy, P.M., Jefferies, J.R., Teesdale-Spittle, P., Tait, A., van Rossum, A.J., LaCourse, E.J., Rijsewijk, F.A.M., Barrett, J. 2004. Binding of hematin to a new class of glutathione transferase from the blood-feeding parasitic nematode Haemonchus contortus. Infection and Immunity pp. 2780-2790.

2003

Chemale, G., van Rossum, A.J., Jefferies, J.R., Barrett, J., Brophy, P.M., Ferreira, H.B., Zaha, A. 2003. Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease. Proteomics pp. 1633-1636.

Hao, Q., Schuller, D.J., Barrett, J., Brophy, P.M., Campbell, A.M., Kriksunov, I.A. 2003. Crystallization and preliminary crystallographic analysis of a new class of glutathione transferase from nematodes. Acta Crystallographica Section D: Biological Crystallography 59 (7) pp. 1262-1264.

2002

Liebau, E., Campbell, A.M., Bergmann, B., Luersen, K., Brophy, P.M., Teesdale-Spittle, P., Walter, R.D. 2002. The glutathione S-transferase from Plasmodium falciparum. Molecular and Biochemical Parasitology pp. 1-2.

2001

Tait, A., Jefferies, J.R., Barrett, J., Brophy, P.M., van Rossum, A.J. 2001. Proteomic identification of glutathione S-transferases from the model nematode Caenorhabditis elegans. Proteomics 1 (11) pp. 1463-1468.

Brophy, P.M., Krause, S., Walter, R.D., Liebau, E., Fischer, P., Sommer, A. 2001. Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans. Molecular and Biochemical Parasitology pp. 145-154.

Barrett, J., Jefferies, J.R., Campbell, A.M., van Rossum, A.J., Brophy, P.M. 2001. Proteomic analysis of Fasciola hepatica excretory-secretory products. Proteomics 1 (9) pp. 1128-1132.

Brophy, P.M., Barrett, J., Saghir, N., Conde, P.J. 2001. Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta. International Journal for Parasitology 31 (7) pp. 653-660.

Campbell, A.M., Brophy, P.M., Barrett, J., Jefferies, J.R., Liebau, E., Teesdale-Spittle, P. 2001. A common class of nematode gluthione S-transferase (GST) revealed by the theoretical proteome of the model organism Caenorhabditis elegans. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology 128 (4) pp. 701-708.

Krause, K., Brophy, P.M., Walter, R.D., Boettcher, K., Liebau, E., Sommer, A., Fischer, P. 2001. A stress-responsive glyoxalase I from the parasitic nematode Onchocerca volvulus. Biochemical Journal 353 pp. 445-452.

2000

Saghir, N., Conde, P.J., Brophy, P., Barrett, J. 2000. A new diagnostic tool for neurocysticercosis is a member of a cestode specific hydrophobic ligand binding protein family. FEBS Letters 487 (2) pp. 181-184. Cadair

Fakae, B.B., Campbell, A.M., Barrett, J., Scott, I., Teesdale-Spittle, P.H., Liebau, E., Brophy, P. 2000. Inhibition of glutathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants. Phytotherapy Research 630-634 (5) pp. 630-634. Cadair

Jefferies, J.R., Brophy, P., Barrett, J. 2000. Investigation of Fasciola hepatica sample preparation for two-dimensional electrophoresis. Electrophoresis 21 (17) pp. 3724-3729. Cadair

Brophy, P., Campbell, A.M., van Eldik, A.J., Teesdale-Spittle, P., Liebau, E., Wang, M.F. 2000. β-Carbonyl substituted glutathione conjugates as inhibitors of O. Volvulus GST2. Bioorganic and Medicinal Chemistry Letters 10 (9) pp. 979-981. Cadair

Adcock, H.J., Brophy, P., Teesdale-Spittle, P.H., Buckberry, L.D. 2000. Purification and characterisation of a novel cysteine conjugate β-lyase from the tapeworm Moniezia expansa. International Journal for Parasitology 30 (5) pp. 567-571. Cadair

Barrett, J., Brophy, P. 2000. Ascaris Haemoglobin: New Tricks for an Old Protein. Parasitology Today 16 (3) pp. 90-91. Cadair