Prof Peter Brophy

PhD

Prof Peter Brophy

Professor

Contact Details

Profile

Studied biochemistry at Glasgow and Stirling Universities before obtaining a PhD from Aberystwyth University on drug metabolism in parasitic worms. After holding a Medical Research Council research post at Aberystwyth (1987-1990) completed further research training in yeast biotechnology at the BioCentre in Leicester. In 1991 was awarded a Wellcome Trust Fellowship based at Nottingham University exploring parasite defences against immune assault. A Senior Research Fellowship in the Leicester Pharmacy School (1994-97) provided opportunities in rational drug design and lead compound synthesis. Established the first proteomics laboratory dedicated to parasitic worm investigations during Readerships at the Universities of Aberystwyth and Liverpool (1998-2006). In 2007 returned to Aberystwyth as a Professor in Parasitology. During  career has been an advisor in parasite drug discovery to WHO and has completed a number of industrial consultancies. To date, has supervised 20 PhD students and published a 100 plus research papers/books/chapters on parasitology, including the first experimental proteomics study in a parasitic worm and  resolved one of the first structures of a parasitic worm drug target Has won research contracts worth over £4 million in order to address  key problems in parasite control (anthelmintic resistance, vaccine, drug  and biomarker discovery).

Teaching

Research

My main research interest is to resolve using functional genomics how liver and rumen fluke parasites interact with their respective host environments. To date, new parasite proteins have been discovered to support drug and vaccine development and diagnostics . I am also interested in developing new molecular tools to measure anthelmintic resistance in liver fluke and a protein biomarker panel has been identified to measure Triclabendazole (TCBZ) failure and resistance for new laboratory and faecal based field tests.

Publications

Tyson, F, Allen, T, Pennington, C, Brophy, P, Thomas, E & Morphew, R 2017, 'Worms at the Royal Welsh Show' Veterinary Record, vol 181, no. 14, pp. 376-377. DOI: 10.1136/vr.j4548
Jones, R, Williams, H, Dalesman, S, Sinmidele, A, Thomas, R & Brophy, P 2017, 'The prevalence and development of digenean parasites within their intermediate snail host, Galba truncatula, in a geographic area where the presence of Calicophoron daubneyi has recently been confirmed' Veterinary Parasitology, vol 240, pp. 68-74. DOI: 10.1016/j.vetpar.2017.03.021
Jones, R, Williams, H & Brophy, P 2017, 'Prevalence of rumen fluke (Calicophoron daubneyi) in cattle herds, sheep flocks and in intermediate snail host, G. truncatula, on Welsh farms' Advances in Animal Biosciences, vol 8, no. 1, pp. 66.
Jones, R, Brophy, P, Mitchell, ES & Williams, H 2017, 'Rumen fluke Calicophoron daubneyi on Welsh farms: Prevalence, risk factors, and observations on co-infection with Fasciola hepatica' Parasitology, vol 144, no. 2, pp. 237-247. DOI: 10.1017/S0031182016001797
Morphew, R, Wilkinson, T, MacKintosh, N, Jahndel, V, Paterson, S, McVeigh, P, Abbas Abidi, SM, Saifullah, K, Raman, M, Ravikumar , G, LaCourse, EJ, Maule, AG & Brophy, P 2016, 'Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species' Journal of Proteome Research, vol 15, no. 9, pp. 3308-3321. DOI: 10.1021/acs.jproteome.6b00331
McCammick, E, McVeigh, P, McCusker, P, Timson, D, Morphew, R, Brophy, P, Marks, N, Mousley, A & Maule, A 2016, 'Calmodulin disruption impacts growth and motility in juvenile liver fluke' Parasites & Vectors, vol 9, 46. DOI: 10.1186/s13071-016-1324-9
Jones, R, Williams, H, Dalesman, S & Brophy, P 2015, 'Confirmation of Galba truncatula as an intermediate host snail for Calicophoron daubneyi in Great Britain, with evidence of alternative snail species hosting Fasciola hepatica' Parasites & Vectors, vol 8, no. 656. DOI: 10.1186/s13071-015-1271-x
Huson, KM, Brophy, P, Morphew, R & MacKintosh, N 2015, 'Identification of the rumen fluke Calicophoron daubneyi infecting cattle in Wales' Advances in Animal Biosciences, vol 6, no. Special Issue 02, pp. 183.
Hart, E, Brophy, P, Prescott, M, Bartley, DJ, Wolf, B & Hamilton, JV 2015, 'A new enabling proteomics methodology to investigate membrane associated proteins from parasitic nematodes: Case study using ivermectin resistant and ivermectin susceptible isolates of Caenorhabditis elegans and Haemonchus contortus' Veterinary Parasitology, vol 207, no. 3-4, pp. 266-275. DOI: 10.1016/j.vetpar.2014.12.003
Ahammed Shareef, PA, Brennan, GP, McVeigh, P, Hannan Khan, MA, Morphew, RM, Mousley, A, Marks, NJ, Khalid Saifullah, MK, Brophy, PM, Maule, AG & Abidi, SMA 2014, 'Time-dependent tegumental surface changes in juvenile Fasciola gigantica in response to triclabendazole treatment in goat' Acta Tropica, vol 136, pp. 108-117. DOI: 10.1016/j.actatropica.2014.04.011
Morphew, RM, MacKintosh, ND, Hart, EH, Prescott, M, LaCourse, EJ & Brophy, PM 2014, 'In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment' EuPA Open Proteomics, vol 3, pp. 85-99. DOI: 10.1016/j.euprot.2014.02.013
McVeigh, P, McCammick, E, McCusker, P, Morphew, RM, Mousley, A, Abidi, A, Saifullah, KM, Muthusamy, R, Gopalakrishnan, R, Spithill, TW, Dalton, JP, Brophy, PM, Marks, NJ & Maule, AG 2014, 'RNAi dynamics in juvenile Fasciola spp. liver flukes reveals the persistence of gene silencing in vitro' PLoS Neglected Tropical Diseases, vol 8, no. 9, 0003185.
Geyer, KK, Chalmers, IW, MacKintosh, ND, Hirst, JE, Geoghegan, R, Badets, M, Brophy, PM, Brehm, K & Hoffmann, KF 2013, 'Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes' BMC Genomics, vol 14, 462. DOI: 10.1186/1471-2164-14-462
Zafra, R, Pérez-Écija, RA, Buffoni, L, Pacheco, IL, Martínez-Moreno, A, Lacourse , EJ, Perally, S, Brophy, PM & Pérez, J 2013, 'Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica.' Research in Veterinary Science, vol 94, no. 3, pp. 602-609. DOI: 10.1016/j.rvsc.2012.10.026
Morphew, RM, Hamilton, CM, Wright, HA, Dowling, DJ, O'Neill, SM & Brophy, PM 2013, 'Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40' Veterinary Parasitology, vol 191, no. 3-4, pp. 379–385. DOI: 10.1016/j.vetpar.2012.08.029
Hart, EH, Morphew, RM, Bartley, DJ, Millares, P, Wolf, BT, Brophy, PM & Hamilton, JV 2012, 'The soluble proteome phenotypes of ivermectin resistant and ivermectin susceptible Haemonchus contortus females compared' Veterinary Parasitology, vol 190, no. 1-2, pp. 104-113. DOI: 10.1016/j.vetpar.2012.06.009
Brophy, PM, Mackintosh, N & Morphew, RM 2012, 'Anthelmintic metabolism in parasitic helminths: proteomic insights' Parasitology, vol 139, no. 9, pp. 1205-1217. DOI: 10.1017/S003118201200087X
LaCourse, EJ, Perally, S, Morphew, RM, Moxon, JV, Prescott, MC, Dowling, DJ, O'Neill, SM, Kipar, A, Hetzel, U, Hoey, E, Zafra, R, Buffoni, L, Arévalo, JP & Brophy, PM 2012, 'The Sigma Class glutathione transferase from the liver fluke Fasciola hepatica' PLoS Neglected Tropical Diseases, vol 6, no. 5, e1666, pp. n/a. DOI: 10.1371/journal.pntd.0001666
Millares, P, LaCourse, J, Perally, S, Ward , D, Prescott, M, Hodgkinson , J, Brophy, PM & Rees, H 2012, 'Proteomic profiling and protein identification by MALDI-TOF mass spectrometry in unsequenced parasitic nematodes.' PLoS One, vol 7, no. 3, e33590. DOI: 10.1371/journal.pone.0033590
Morphew, RM, Eccleston, N, Wilkinson, TJ, McGarry, J, Perally, S, Prescott, M, Ward, D, Williams, D, Paterson, S, Raman, M, Ravikumar, G, Khalid Saifullah, M, Abbas Abidi, SM, McVeigh, P, Maule, AG, Brophy, PM & LaCourse, EJ 2012, 'Proteomics and in silico approaches to extend understanding of the glutathione transferase superfamily of the tropical liver fluke Fasciola gigantica' Journal of Proteome Research, vol 11, no. 12, pp. 5876–5889. DOI: 10.1021/pr300654w
Morphew, RM, Wright, HA, LaCourse, EJ, Porter, J, Barrett, J, Woods, DJ & Brophy, PM 2011, 'Towards delineating functions within the fasciola secreted cathepsin L protease family by integrating in vivo based sub-proteomics and phylogenetics' PLoS Neglected Tropical Diseases, vol 5, no. 1, e-937. DOI: 10.1371/journal.pntd.0000937
Mendes, RE, Zafra, R, Martinez-Moreno, A, Brophy, P & Pérez, J 2010, 'Early Hepatic Changes in Goats Immunized with Recombinant Glutathione S Transferase (Sigma Class) and Challenged with Fasciola hepatica' Journal of Comparative Pathology, vol 143, no. 4, pp. 350. DOI: 10.1016/j.jcpa.2010.09.119
Chemale, G, Perally, S, LaCourse, EJ, Prescott, MC, Jones, LM, Ward, D, Meany, M, Hoey, E, Brennan, GP, Fairweather, I, Trudgett, A & Brophy, PM 2010, 'Comparative proteomic analysis of Triclabendazole response in the liver fluke Fasciola hepatica' Journal of Proteome Research, vol 9, no. 10, pp. 4940-4951. DOI: 10.1021/pr1000785
Moxon, JV, LaCourse, EJ, Wright, HA, Perally, S, Prescott, MC, Gillard, JL, Barrett, J, Hamilton, JV & Brophy, PM 2010, 'Proteomic analysis of embryonic Fasciola hepatica: Characterization and antigenic potential of a developmentally regulated heat shock protein' Veterinary Parasitology, vol 169, no. 1-2, pp. 62-75. DOI: 10.1016/j.vetpar.2009.12.031
Jones, LM, Staffa, K, Perally, S, LaCourse, EJ, Brophy, PM & Hamilton, JV 2010, 'Proteomic analyses of Caenorhabditis elegans dauer larvae and long-lived daf-2 mutants implicates a shared detoxification system in longevity assurance' Journal of Proteome Research, vol 9, no. 6, pp. 2871-2881. DOI: 10.1021/pr9009639
Zhan, B, Perally, S, Brophy, PM, Xue, J, Goud, G, Liu, S, Deumic, V, de Oliveira, L, Bethony, J, Bottazzi, ME, Jiang, D, Gillespie, P, Xiao, S, Gupta, R, Loukas, A, Ranjit, N, Lustigman, S, Hotez, P & Oksov, Y 2010, 'Molecular Cloning, Biochemical Characterization, and Partial Protective Immunity of the Heme-Binding Glutathione S-Transferases from the Human Hookworm Necator americanus' Infection and Immunity, vol 78, no. 4, pp. 1552-1563. DOI: 10.1128/IAI.00848-09
Moxon, JV, Flynn, R, Golden, O, Hamilton, JV, Mulcahy, G & Brophy, PM 2010, 'Immune responses directed at egg proteins during experimental infection with the liver fluke Fasciola hepatica' Parasite Immunology, vol 32, no. 2, pp. 111-124. DOI: 10.1111/j.1365-3024.2009.01171.x
Dowling, DJ, Hamilton, CM, Donnelly, S, La Course, J, Brophy, PM, Dalton, J & O'Neill, SM 2010, 'Major secretory antigens of the helminth Fasciola hepatica activate a suppressive dendritic cell phenotype that attenuates Th17 cells but fails to activate Th2 immune responses' Infection and Immunity, vol 78, no. 2, pp. 793-801. DOI: 10.1128/IAI.00573-09
Hamilton, CM, Dowling, DJ, Loscher, CE, Morphew, RM, Brophy, PM & O'Neill, SM 2009, 'The Fasciola hepatica tegumental antigen suppresses dendritic cell maturation and function' Infection and Immunity, vol 77, no. 6, pp. 2488-2498. DOI: 10.1128/IAI.00919-08
LaCourse, EJ, Hernandez-Viadel, M, Jefferies, JR, Svendsen, C, Spurgeon, DJ, Barrett, J, Morgan, AJ, Kille, P & Brophy, PM 2009, 'Glutathione transferase (GST) as a candidate molecular based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus' Environmental Pollution, vol 157, no. 8-9, pp. 2459-2469. DOI: 10.1016/j.envpol.2009.03.015
Perally, S, LaCourse, EJ, Campbell, AM & Brophy, PM 2008, 'Heme transport and detoxification in nematodes: subproteomics evidence of differential role of glutathione transferases' Journal of Proteome Research, vol 7, no. 10, pp. 4557-4565. DOI: 10.1021/pr800395x
LaCourse, EJ, Perally, S, Hernandez-Viadel, M, Wright, HA & Brophy, PM 2008, 'A Proteomics Approach To Quantify Protein Levels Following RNA Interference: Case Study with Glutathione Transferase Superfamily from the Model Metazoan Caenorhabditis elegans' Journal of Proteome Research, vol 7, no. 8, pp. 3314-3318. DOI: 10.1021/pr8001035
Somboon, P, Severson, DW, Lumjuan, N, Stevenson, BJ, Prapanthadara, L, Ranson, H, Brophy, PM & Loftus, BJ 2007, 'The Aedes aegypti glutathione transferase family' Insect Biochemistry and Molecular Biology, vol 37, no. 10, pp. 1026-1035. DOI: 10.1016/j.ibmb.2007.05.018
Morphew, RM, Wright, HA, LaCourse, EJ, Woods, DJ & Brophy, PM 2007, 'Comparative proteomics of excretory-secretory proteins released by the liver fluke Fasciola hepatica in sheep host bile and during in vitro culture ex host' Molecular and Cellular Proteomics, vol 6, pp. 963-972. DOI: 10.1074/mcp.M600375-MCP200
Brennan, GP, Fairweather, I, Trudgett, A, Hoey, E, ., M, Mcconville, M, Meaney, M, Robinson, M, McFerran, N, Ryan, L, Lanusse, C, Mottier, L, Alvarez, L, Solana, H, Virkel, G & Brophy, P 2007, 'Understanding triclabendazole resistance' Experimental and Molecular Pathology, vol 82, no. 2, pp. 104-109. DOI: 10.1016/j.yexmp.2007.01.009
Chemale, G, Morphew, RM, Moxon, JV, Morassuti, AL, LaCourse, EJ, Barrett, J, Johnston, DA & Brophy, PM 2006, 'Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica' Proteomics, vol 6, no. 23, pp. 6263-6273. DOI: 10.1002/pmic.200600499
Morgan, CT, LaCourse, EJ, Rushbrook, BJ, Greetham, D, Hamilton, JV, Barrett, J, Bailey, K & Brophy, PM 2006, 'Plasticity demonstrated in the proteome of a parasitic nematode within the intestine of different host strains' Proteomics, vol 6, no. 16, pp. 4633-4645. DOI: 10.1002/pmic.200600068
Morphew, RM, Barrett, J & Brophy, PM 2006, Flatworm parasite proteomics. in J N (ed.), in. CABI Publishing, pp. 327-.
Schuller, DJ, Liu, Q, Kriksunov, IA, Campbell, AM, Barrett, J, Brophy, PM & Hao, Q 2005, 'Crystal structure of a new class of glutathione transferase from the model human hookworm nematode Heligmosomoides polygyrus' Proteins: Structure, Function and Genetics, vol 61, no. 4, pp. 1024-1031. DOI: 10.1002/prot.20649
Zhan, B, Liu, S, Perally, S, Xue, J, Fujiwara, R, Brophy, PM, Xiao, S, Liu, Y, Feng, J, Williamson, A, Wang, Y, Bueno, LL, Mendez, S, Goud, G, Bethony, JM, Hawdon, JM, Loukas, A, Jones, K & Hotez, PJ 2005, 'Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum' Infection and Immunity, vol 73, no. 10, pp. 6903-6911. DOI: 10.1128/IAI.73.10.6903-6911.2005
Barrett, J, Brophy, PM & Hamilton, JV 2005, 'Analysing proteomic data' International Journal for Parasitology, vol 35, no. 5, pp. 543-553. DOI: 10.1016/j.ijpara.2005.01.013
Chemale, G, Barrett, J, Zaha, A, Ferreira, HB & Brophy, PM 2005, 'Echinococcus granulosus antigen B hydrophobic ligand binding properties' Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, pp. 189-194. DOI: 10.1016/j.bbapap.2004.11.004
Greetham, D, Morgan, CT, Campbell, AM, van Rossum, AJ, Barrett, J & Brophy, PM 2004, 'Evidence of glutathione transferase complexing and signaling in the model nematode Caenorhabditis elegans using a pull-down proteomic assay' Proteomics, vol 4, no. 7, pp. 1989-1995. DOI: 10.1002/pmic.200300719
Brophy, PM, Jefferies, JR, Teesdale-Spittle, P, Tait, A, van Rossum, AJ, LaCourse, EJ, Rijsewijk, FAM & Barrett, J 2004, 'Binding of hematin to a new class of glutathione transferase from the blood-feeding parasitic nematode Haemonchus contortus.' Infection and Immunity, pp. 2780-2790. DOI: 10.1128/IAI.72.5.2780-2790.2004
Chemale, G, van Rossum, AJ, Jefferies, JR, Barrett, J, Brophy, PM, Ferreira, HB & Zaha, A 2003, 'Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease' Proteomics, pp. 1633-1636. DOI: 10.1002/pmic.200300487
Hao, Q, Schuller, DJ, Barrett, J, Brophy, PM, Campbell, AM & Kriksunov, IA 2003, 'Crystallization and preliminary crystallographic analysis of a new class of glutathione transferase from nematodes' Acta Crystallographica Section D: Biological Crystallography, vol 59, no. 7, pp. 1262-1264. DOI: 10.1107/S0907444903009041
Liebau, E, Campbell, AM, Bergmann, B, Luersen, K, Brophy, PM, Teesdale-Spittle, P & Walter, RD 2002, 'The glutathione S-transferase from Plasmodium falciparum' Molecular and Biochemical Parasitology, pp. 1-2. DOI: 10.1016/S0166-6851(02)00160-3
Tait, A, Jefferies, JR, Barrett, J, Brophy, PM & van Rossum, AJ 2001, 'Proteomic identification of glutathione S-transferases from the model nematode Caenorhabditis elegans' Proteomics, vol 1, no. 11, pp. 1463-1468. DOI: 10.1002/1615-9861(200111)1:11<1463::AID-PROT1463>3.0.CO;2-H
Brophy, PM, Krause, S, Walter, RD, Liebau, E, Fischer, P & Sommer, A 2001, 'Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans' Molecular and Biochemical Parasitology, pp. 145-154. DOI: 10.1016/S0166-6851(01)00342-5
Barrett, J, Jefferies, JR, Campbell, AM, van Rossum, AJ & Brophy, PM 2001, 'Proteomic analysis of Fasciola hepatica excretory-secretory products' Proteomics, vol 1, no. 9, pp. 1128-1132. DOI: 10.1002/1615-9861(200109)1:9<1128::AID-PROT1128>3.0.CO;2-0
Brophy, PM, Barrett, J, Saghir, N & Conde, PJ 2001, 'Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta' International Journal for Parasitology, vol 31, no. 7, pp. 653-660. DOI: 10.1016/S0020-7519(01)00135-7
Campbell, AM, Brophy, PM, Barrett, J, Jefferies, JR, Liebau, E & Teesdale-Spittle, P 2001, 'A common class of nematode gluthione S-transferase (GST) revealed by the theoretical proteome of the model organism Caenorhabditis elegans' Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, vol 128, no. 4, pp. 701-708. DOI: 10.1016/S1096-4959(00)00360-2
Krause, K, Brophy, PM, Walter, RD, Boettcher, K, Liebau, E, Sommer, A & Fischer, P 2001, 'A stress-responsive glyoxalase I from the parasitic nematode Onchocerca volvulus' Biochemical Journal, vol 353, pp. 445-452.
Saghir, N, Conde, PJ, Brophy, P & Barrett, J 2000, 'A new diagnostic tool for neurocysticercosis is a member of a cestode specific hydrophobic ligand binding protein family' FEBS Letters, vol 487, no. 2, pp. 181-184. DOI: 10.1016/S0014-5793(00)02191-8
Fakae, BB, Campbell, AM, Barrett, J, Scott, I, Teesdale-Spittle, PH, Liebau, E & Brophy, P 2000, 'Inhibition of glutathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants' Phytotherapy Research, vol 630-634, no. 5, pp. 630-634. DOI: 10.1002/1099-1573(200012)14:8<630::AID-PTR773>3.0.CO;2-5
Jefferies, JR, Brophy, P & Barrett, J 2000, 'Investigation of Fasciola hepatica sample preparation for two-dimensional electrophoresis' Electrophoresis, vol 21, no. 17, pp. 3724-3729. DOI: 10.1002/1522-2683(200011)21:17<3724::AID-ELPS3724>3.0.CO;2-K
Brophy, P, Campbell, AM, van Eldik, AJ, Teesdale-Spittle, P, Liebau, E & Wang, MF 2000, 'β-Carbonyl substituted glutathione conjugates as inhibitors of O. Volvulus GST2' Bioorganic and Medicinal Chemistry Letters, vol 10, no. 9, pp. 979-981. DOI: 10.1016/S0960-894X(00)00142-6
Adcock, HJ, Brophy, P, Teesdale-Spittle, PH & Buckberry, LD 2000, 'Purification and characterisation of a novel cysteine conjugate β-lyase from the tapeworm Moniezia expansa' International Journal for Parasitology, vol 30, no. 5, pp. 567-571. DOI: 10.1016/S0020-7519(00)00024-2
Barrett, J & Brophy, P 2000, 'Ascaris Haemoglobin: New Tricks for an Old Protein' Parasitology Today, vol 16, no. 3, pp. 90-91. DOI: 10.1016/S0169-4758(99)01613-0